agar and Lymphoma--Non-Hodgkin

Topics: Agar; Animals; Child; Culture Media; Culture Techniques; Hodgkin Disease; Humans; Inclusion Bodies, Viral; Leukemia; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Male; Mice; Microscopy, Electron; Mycoplasma; Neoplasms; Neoplasms, Experimental; Rabbits; Rats

This study compared T-cell colony formation in soft agar of lymphocytes from the peripheral blood and lymph nodes of patients with non-Hodgkin's lymphoma (NHL) with T-cell colony formation of peripheral blood lymphocytes from normal individuals. Mononuclear cells were separated from other blood and lymph node elements on density gradient columns, phenotyped for T- and B-cell antigens using monoclonal antibodies, and then plated in soft agar cultures. Lymphocytes from peripheral blood and lymph nodes of patients with NHL exhibited less T-cell colony formation (p less than 0.01) than did lymphocytes from normal individuals. This decrease in T-cell colony formation was not the result of the number of T cells or null cells plated, or differences in proportions of T helper and T suppressor cells. However, when sera from patients with NHL were incubated with normal lymphocytes before plating in soft agar, a decrease in number of T-cell colonies was observed (p less than 0.01). We conclude that peripheral blood and lymph node mononuclear cells from patients with NHL have a decreased ability to form T-cell colonies in soft agar cultures and that this decrease is related, at least in part, to the presence of serum factor(s).

Topics: Agar; Antibodies, Monoclonal; Blood Proteins; Colony-Forming Units Assay; Hematopoiesis; Humans; Lymph Nodes; Lymphocyte Activation; Lymphoma, Non-Hodgkin; Phenotype; Phytohemagglutinins; T-Lymphocytes; T-Lymphocytes, Helper-Inducer; T-Lymphocytes, Regulatory

Bone marrow cells from BALB/c mice with myeloid leukemia, lymphosarcoma, erythroblastosis, or mammary tumor produce small clusters in semisolid agar cultures in the absence of specific colony-stimulating factor. This spontaneous growth is observed only when high cell numbers (5 x 10 5 cells/ml) are plated. The phenomenon was encountered only when mice had an elevated number of mature or immature granulocytes in the peripheral blood. Removal of the adherent cells from the bone marrow did not abolish spontaneous growth, indicating that this colony-stimulating factor independency is not due to a high number of colony-stimulating cells in the bone marrow cells. This excluded the possibility that the spontaneous growth was due to a high endogenous stimulating activity of the bone marrow from tumor-bearing mice.

Topics: Agar; Animals; Bone Marrow; Bone Marrow Cells; Cell Division; Clone Cells; Granulocytes; Leukemia, Myeloid; Leukocyte Count; Lymphoma, Non-Hodgkin; Mammary Neoplasms, Experimental; Mice; Mice, Inbred BALB C; Neoplasms, Experimental; Rauscher Virus; Sarcoma, Experimental

Topics: Agar; Animals; Chromatography; Electrophoresis; Erythrocytes; Gels; Hemolysin Proteins; Hydroxyapatites; Immunization; Lymphoma, Non-Hodgkin; Neoplasm Transplantation; Neoplasms, Experimental; Rats; RNA; Sheep; Spleen

Topics: Adenocarcinoma; Adenocarcinoma, Scirrhous; Adolescent; Adult; Agar; Aged; Carcinoma; Chromatography; Colonic Neoplasms; Gallbladder Neoplasms; Hemangiosarcoma; Humans; Liver Neoplasms; Lymphoma, Non-Hodgkin; Middle Aged; Neoplasm Proteins; Neoplasm Recurrence, Local; Pancreatic Neoplasms; Protein Denaturation; Retroperitoneal Neoplasms; Stomach Neoplasms

Topics: Agar; Animals; Animals, Laboratory; Arthritis, Infectious; Cricetinae; Culture Media; Culture Techniques; Disease Models, Animal; Freund's Adjuvant; Guinea Pigs; Injections; Injections, Intraperitoneal; Lymphoma, Non-Hodgkin; Methylcellulose; Mice; Mucins; Mycoplasma; Mycoplasma Infections; Rabbits; Rats; Sarcoma, Experimental; Sepsis; Silicon Dioxide; Talc; Virulence

Topics: Agar; Aged; Electrophoresis; Gels; Humans; Immunoelectrophoresis; Lymphoma, Non-Hodgkin; Male; Nephrotic Syndrome; Pelvic Neoplasms; Waldenstrom Macroglobulinemia

Topics: Agar; Animals; Bacteria; Cattle; Cattle Diseases; Culture Media; Culture Techniques; Fluorescent Antibody Technique; Globulins; Goats; Kidney; Lymph Nodes; Lymphoma, Non-Hodgkin; Microscopy; Rabbits; Staining and Labeling

Topics: Agar; Alkaline Phosphatase; Bile; Bone Diseases; Bone Neoplasms; Child; Clinical Enzyme Tests; Electrophoresis; Hodgkin Disease; Humans; Jaundice; Jaundice, Obstructive; Liver Diseases; Liver Neoplasms; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Osteitis Deformans; Sarcoma