agar and Lymphoma--Large-B-Cell--Diffuse

Topics: Agar; Animals; Child; Culture Media; Culture Techniques; Hodgkin Disease; Humans; Inclusion Bodies, Viral; Leukemia; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Male; Mice; Microscopy, Electron; Mycoplasma; Neoplasms; Neoplasms, Experimental; Rabbits; Rats

Tumor-bearing lymph nodes from SJL mice were characterized by histologic, ultrastructural, and immunologic methods. These approaches consistently revealed a predominance of macrophage-like cells in the primary neoplasm. When the tumor-bearing lymph nodes were placed in cell culture, colonies of adherent cells grew slowly to confluence and demonstrated morphologic and functional properties of macrophages. The tumor cells were also grown in soft agar where clusters and colonies of large, often binucleate, cells predominated. These cells were uniformly nonspecific esterase-positive, again, suggesting a macrophage origin. In addition, supernatants derived from SJL tumor cells were shown to have mitogen-augmenting activity as tested on murine thymocytes. These findings are discussed in the context of the SJL tumor as a proliferative condition primarily involving macrophages, which may be useful as a model of human diseases such as Hodgkin's disease.

Topics: Agar; Animals; Culture Techniques; Female; Interleukin-1; Interleukin-2; Lymph Nodes; Lymphatic Diseases; Lymphoma, Large B-Cell, Diffuse; Macrophages; Mice; Mitogens; Neoplasms; Proteins

Topics: Agar; Anemia, Hypochromic; Anemia, Pernicious; Clinical Enzyme Tests; Electrophoresis; Female; Hodgkin Disease; Humans; Isoenzymes; L-Lactate Dehydrogenase; Lymphoma, Large B-Cell, Diffuse; Male; Neoplasms

Topics: Agar; Alkaline Phosphatase; Bile; Bone Diseases; Bone Neoplasms; Child; Clinical Enzyme Tests; Electrophoresis; Hodgkin Disease; Humans; Jaundice; Jaundice, Obstructive; Liver Diseases; Liver Neoplasms; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Osteitis Deformans; Sarcoma