agar and Liver-Cirrhosis

Spontaneous bacterial peritonitis is a life-threatening complication of cirrhotic ascites. Optimal patient management depends on the isolation of the causal organism from ascitic fluid. To evaluate culture techniques for the diagnosis of spontaneous bacterial peritonitis, we prospectively compared three blood culture system, the Isolator system, a lysis-centrifugation system, the Septi-Chek system, a biphasic culture system, and a nonvented tryptic soy broth system, all inoculated at the bedside, and our standard method of direct inoculation of specimens after transport to the laboratory onto agar plates and into thioglycolate broth. The results showed that the Septi-Chek and nonvented tryptic soy broth systems each recovered statistically significantly more pathogens than either the Isolator system (P = 0.0084) or the standard method (P = 0.00098). The Isolator system recovered more pathogens than the standard plate method, but this difference was not statistically significant. Both the Isolator system and the standard plate method recovered more contaminating microorganisms than the Septi-Chek or nonvented tryptic soy broth system. The Isolator system required the most processing time compared with the processing times required by any other method.

Topics: Adult; Agar; Aged; Aged, 80 and over; Bacterial Infections; Bacteriological Techniques; Culture Media; Evaluation Studies as Topic; Female; Humans; Liver Cirrhosis; Male; Middle Aged; Peritonitis; Prospective Studies; Time Factors

Blood granulocyte-macrophage progenitor cells (CFU-GM) and myeloid differentiation were studied in 16 patients with hepatic cirrhosis by culturing blood mononuclear cells in semisolid and in liquid medium. The numbers of CFU-GM from normal and cirrhotic subjects were not significantly different, even when increased numbers of monocyte-macrophagic colonies and decreased numbers of granulocytic colonies were observed in cirrhotic patients. Significantly reduced granulocytic growth and increased monocyte-macrophagic cell growth were found in liquid culture of cirrhotic patients. These data seem to indicate that in hepatic cirrhosis, besides granulocyte sequestration within the spleen, there is a disorder of granulocytic versus monocyte-macrophagic differentiation.

Topics: Adult; Agar; Aged; Cell Count; Cell Differentiation; Cell Separation; Cells, Cultured; Culture Media; Female; Granulocytes; Hematopoietic Stem Cells; Humans; Liver Cirrhosis; Macrophages; Male; Middle Aged

Topics: Agar; Animals; Blood Protein Electrophoresis; Electrophoresis; Humans; Immunoelectrophoresis; Immunoglobulin M; Liver Cirrhosis; Molecular Weight; Osmosis; Polysaccharides; Rabbits; Waldenstrom Macroglobulinemia

Topics: Agar; Blood Protein Electrophoresis; Electrophoresis, Paper; Haptoglobins; Humans; Immunoelectrophoresis; Liver Cirrhosis

Topics: Acute Disease; Adolescent; Adult; Agar; Age Factors; Child; Child, Preschool; Chronic Disease; Fatty Liver; Female; Follow-Up Studies; Hepatitis A; Hepatitis B; Hepatitis B Antigens; Hepatovirus; Humans; Immunodiffusion; Immunoelectrophoresis; Infant; Liver Cirrhosis; Liver Diseases; Liver Regeneration; Male; Middle Aged; Sex Factors

Topics: Agar; Animals; Biopsy; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Electrophoresis; Gels; Hepatitis; Histocytochemistry; Humans; Liver Cirrhosis; Liver Diseases; Proteins; Rabbits; Rats; Thioacetamide

Topics: Acyltransferases; Agar; Bile Duct Neoplasms; Biliary Tract Diseases; Cholelithiasis; Electrophoresis; Glutamates; Hepatitis; Humans; Isoenzymes; Jaundice; Liver Cirrhosis; Liver Diseases; Liver Neoplasms; Pancreatic Diseases; Pancreatic Neoplasms; Pancreatitis

Topics: Acute Disease; Agar; Alanine; Alkaline Phosphatase; Amides; Aminohydrolases; Anilides; Cholestasis; Chronic Disease; Electrophoresis; Hepatitis; Humans; Isoenzymes; Leucine; Liver Cirrhosis; Liver Diseases; Liver Neoplasms; Naphthalenes; Nitro Compounds; Pancreatic Diseases; Pancreatitis

Topics: Agar; Cellulose; Cholestasis; Diabetes Mellitus; Electrophoresis; Follow-Up Studies; Gels; Hepatitis; Humans; Lipoproteins; Liver Cirrhosis; Medical Records; Methods; Nephrosis

Topics: Agar; Aspergillosis; Asthma; Celiac Disease; Eczema; Food Hypersensitivity; gamma-Globulins; Hemosiderosis; Humans; Immunodiffusion; Immunoglobulin G; Immunoglobulin M; Larva Migrans, Visceral; Liver Cirrhosis; Lung Diseases; Methods; Multiple Myeloma; Rhinitis, Allergic, Seasonal

Topics: Agar; Alcoholism; Diabetes Complications; Fatty Liver; gamma-Globulins; Hepatitis; Humans; Immunodiffusion; Immunoglobulin G; Immunoglobulin M; Liver Cirrhosis; Liver Diseases; Liver Function Tests; Methods; Time Factors

Topics: Agar; Animals; Arthritis, Rheumatoid; Beta-Globulins; Complement System Proteins; Hemolysis; Humans; Hydrazines; Immune Sera; Immunoelectrophoresis; Liver Cirrhosis; Multiple Myeloma; Precipitins; Stomach Neoplasms

Topics: Acrylates; Agar; Aminocaproates; Animals; Blood Coagulation Disorders; Blood Coagulation Tests; Electrophoresis; Extracorporeal Circulation; Fibrinogen; Fibrinolysin; Fibrinolysis; Fibrinolytic Agents; Gels; Humans; Immune Sera; Immunoelectrophoresis; Liver Cirrhosis; Male; Molecular Weight; Nicotinic Acids; Plasma; Prostatic Neoplasms; Rabbits

Topics: Agar; Animals; gamma-Globulins; Goats; Humans; Immune Sera; Immunoelectrophoresis; Liver Cirrhosis; Polysaccharides; Rabbits; Transferrin; Waldenstrom Macroglobulinemia

Topics: Agar; Blood Protein Disorders; Blood Protein Electrophoresis; Humans; Immunoelectrophoresis; Liver Cirrhosis; Methods

Topics: Agar; Electrophoresis; Humans; Liver; Liver Cirrhosis; Proteins