agar and Leukemia--Myeloid--Acute

The technique of bone marrow cultures has been shown to be of value in childhood acute leukemia. It now appears that acute myelogenous leukemia may be due to defective maturation of normal progenitor cells. The pattern of growth of these cells has been demonstrated to be of prognostic value. In contrast, the growth of normal progenitor cells from the bone marrow cultures of children with acute lymphocytic leukemia (ALL) may be due to the few remaining normal cells. The cause of granulocytopenia in childhood ALL is still unclear.

Topics: Acute Disease; Agar; Bone Marrow; Cell Transformation, Neoplastic; Cells, Cultured; Child; Child, Preschool; Colony-Stimulating Factors; Culture Media; Granulocytes; Hematopoiesis; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Phytohemagglutinins

The in vitro cloning of haemopoietic precursors is a rapidly growing field. The data reviewed above and the current practical applications of the techniques can be expected to increase quite rapidly in the next decade. Despite the technical problems of tissue culture and the special problems associated with culturing human cells, it is clear that these procedures can be effectively applied at the clinical level. The value of the data obtained will vary directly with the quality of the culture techniques. Any centre undertaking these techniques must be prepared to properly equip the culture laboratory, appoint a full-time staff member for the work and maintain a constant surveillance of the quality of the culture work.

Topics: Agar; Anemia, Aplastic; Cells, Cultured; Colony-Forming Units Assay; Colony-Stimulating Factors; Culture Media; Granulocytes; Hematopoietic Stem Cells; Humans; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Monocytes; Myeloproliferative Disorders; Polycythemia Vera; Preleukemia; Primary Myelofibrosis; Time Factors

Topics: Agar; Animals; Antigens; Blood; Bone Marrow Cells; Cell Division; Clone Cells; Culture Techniques; Humans; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Macrophages; Methods; Mice

The distinction of clonogenic leukemic cells (CFU-L) and normal myeloid progenitors (GM-CFU) is a problem because both types of cells respond to the same growth factors and their clones resemble each other morphologically in culture. We investigated by means of an indirect enzyme-immunoassay the expression of "early" and "late" differentiation markers on bone marrow cell suspensions, as well as on agar clones in 18 cases of newly diagnosed acute myeloid leukemia (AML) as compared with 13 normal controls. Uncultured AML cells carried only low amounts of "late" myeloid differentiation antigen (CD15) but expressed nearly normal levels when cultured in agar with colony-stimulating factor (CSF). In contrast to normal bone marrow, AML cells were strongly reactive with "early" differentiation markers (CD10, CD20, CD34) and remained so during culture. Normal and leukemic agar clones could be specifically distinguished by CD20- and CD34 antibodies. By means of a double marker technique, it could be shown that "late" myeloid differentiation markers (CD15) and "early" markers (CD10, CD20, CD34) were coexpressed on the same cells only in AML but not in normal bone marrow. Leukemic clones were identified by phenotyping of agar clones in 17 of 19 cases investigated during complete clinical remission (CR) of the disease. A formal proof of the leukemic origin of CD20/CD34 positive clones grown in CR was made possible in four cases either by Southern blot analysis or by a cytogenetic marker. These results demonstrate that AML cells can partially differentiate in vitro in the presence of CSF. A distinction of AML from normal clones, however, is possible by their reactivity with "early" differentiation markers, because this is maintained under the differentiating influence of CSF. The technique described here identifies residual leukemic clones in the majority of AML in CR, which persist at a constant rate and increase 6 months before cytological relapse.

Topics: Agar; Antibodies; Antigens, CD20; Antigens, CD34; Antigens, Differentiation; Antigens, Differentiation, B-Lymphocyte; Antigens, Neoplasm; Antigens, Surface; Blotting, Western; Bone Marrow; Bone Marrow Cells; Clone Cells; Humans; Leukemia, Myeloid, Acute; Leukocytes, Mononuclear; Lewis X Antigen; Neprilysin

In the present study, the growth and differentiation capacity of myeloid leukemic cells in agar and liquid cultures have been investigated in relation to their prognostic significance for treatment outcome and early detection of relapse. Prior to induction therapy, leukemic cells failed to differentiate and the colony or cluster number did not correlate with response to treatment. Seventeen to 42 days after induction, patients with BM cells producing greater than 10 colonies or greater than 30 clusters resp. had a high likelihood of achieving a complete remission. Cells from refractory patients had a significantly impaired differentiation capacity. During remission, a colony number greater than 50 was significantly associated with a high probability to remain in further remission for greater than 3 months. An impaired differentiation was significantly associated with the likelihood of relapsing within 3 months. In the light of these results, agar and liquid cultures appear to be useful for monitoring the effect of induction chemotherapy and detecting patients likely to relapse.

Topics: Agar; Antineoplastic Agents; Bone Marrow; Cell Differentiation; Cells, Cultured; Culture Media; Humans; Leukemia, Myeloid, Acute; Prognosis

Cord plasma contains colony-stimulating activity (CSA) which stimulates the in vitro clonal growth of neutrophils, eosinophils, macrophages, erythrocytes, and persisting mast cells in semisolid cultures. Analysis of day 35 colonies in agar cultures was found to be a suitable means of demonstrating this activity and discriminating between it and granulocyte-macrophage colony-stimulating factor (GM-CSF). Serum (10%) from patients with acute and chronic myeloid leukemia (AML and CML) was added to normal human bone marrow cultures to search for similar activity in these patient's serum. Although the number of colonies on day 12 (predominantly neutrophils and macrophages) was not significantly different from the number of colonies in cultures containing normal serum, the number of colonies increased 500% in cultures containing CML serum on day 35. Serum from patients with AML during regeneration also stimulated an increased number of colonies on day 35. Although both eosinophil and mast cell colonies were still present on day 35, only mast cell colonies persisted for 150 days. On day 35, cultures containing 10% CML serum contained predominantly eosinophil colonies (84%), whereas cultures containing AML serum contained predominantly mast cell colonies (76%). Although serum contains various CSFs, the specific factor which stimulates persisting mast cell colonies may be the human equivalent of murine persisting (P) cell-stimulating factor (Multi-CSF).

Topics: Agar; Cells, Cultured; Colony-Forming Units Assay; Colony-Stimulating Factors; Fetal Blood; Granulocytes; Hematopoietic Stem Cells; Humans; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Macrophages

Marrow or peripheral blood cells from 28 patients with acute myeloid leukemia (AML) or chronic myeloid leukemia in blastic crisis (CML-BC) were studied in both liquid and agar cultures. The proliferation and maturation of these cells were followed for 15-20 days in liquid culture with or without the addition of human placenta conditioned medium (HPCM) and/or retinoic acid (RA). In nine patients (group 1), cells underwent both proliferation and maturation, i.e., the percentage of peroxidase-positive cells (PO), phagocytic cells, and mature forms increased. For the remaining 19 patients (group 2), no proliferation was observed. However, 11 of these leukemic cell samples showed maturation (group 2A), while the eight others remained immature (group 2B). In agar culture, cell samples from group 1 showed cluster growth, group 2 no growth. Maturation without proliferation was observed for group-1 liquid cultures not containing HPCM and those containing HPCM and RA. The viability rapidly decreased in liquid cultures with only addition of RA. HPCM and RA showed no effect on group-2 cell cultures.

Topics: Adult; Agar; Aged; Bone Marrow; Cell Division; Cells, Cultured; Child; Culture Media; Culture Techniques; Follow-Up Studies; Humans; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Middle Aged; Phagocytosis; Placenta

To investigate the mechanisms supporting the in vitro longterm growth of murine leukemic myeloblastic cells, factor-dependent and autonomous myeloblastic cells have been examined for their CSF responsiveness, CSF secretion, and autostimulation of growth. Purified CSF-1, GM-CSF, and IL-3 stimulated cloning and proliferation of both autonomous (ACL) and factor-dependent cell lines (FDCL), and were unable to induce differentiation of these cells. Sensitivities to CSFs were similar for ACL, FDCL and normal bone marrow cells. Most of the cell lines secreted CSFs, stimulating colony formation from normal bone marrow cells in bilayer agar cultures. The number of induced granulo-macrophagic colonies was similar in the presence of either ACL or FDCL. The ability to stimulate their own proliferation was similar for both ACL and FDCL in a longterm suspension culture assay as well as in a shortterm cloning assay. These results strongly suggest that the autocrine secretion of CSF-related activities are not sufficient to fully account for the in vitro longterm proliferation of virus-transformed myelomonocytic cells.

Topics: Agar; Bone Marrow Cells; Cell Differentiation; Cell Division; Cell Line; Colony-Forming Units Assay; Colony-Stimulating Factors; Granulocytes; Humans; Interleukin-3; Leukemia, Myeloid, Acute; Lung; Lymphokines; Macrophages; Thymidine

Topics: Agar; Antineoplastic Agents; Cell Survival; Clone Cells; Colony-Forming Units Assay; Culture Techniques; DNA Replication; Drug Evaluation, Preclinical; Humans; Immunoglobulin A; Immunoglobulin G; Interferon Type I; Leukemia, Myeloid, Acute; Multiple Myeloma; Tumor Stem Cell Assay

Human acute myeloid leukemia (ML-1 and HL-60) cells grew continuously in the serum-free liquid medium supplemented with human transferrin and bovine insulin. Both ML-1 and HL-60 cells formed clusters and colonies in the serum-free agar medium supplemented with bovine serum albumin, human transferrin, cholesterol, and L-alpha-phosphatidylcholine. Medium conditioned by phytohemagglutinin-stimulated leukocytes prepared in the absence of serum had three types of colony-stimulating factors on normal human bone marrow cells. When fetal calf serum was present, medium conditioned by phytohemagglutinin-stimulated leukocytes stimulated the clonal growth of HL-60 cells at the lower concentration. However, it inhibited that of ML-1 cells. In contrast, under serum-free conditions, medium conditioned by phytohemagglutinin-stimulated leukocytes promoted the clonal growth of both ML-1 and HL-60 cells at the lower concentrations. The study using a Sephadex G-200 column revealed that, in the serum-supplemented cultures, HL-60 cells responded to one of the three colony-stimulating factors and an activity with molecular weight of around 12,000, while ML-1 cells responded only to an activity with molecular weight of around 12,000. In the serum-free cultures, both ML-1 and HL-60 cells were stimulated by activities with molecular weights of 62,000 and 54,000, respectively. These studies demonstrate that the determination of growth factors for cell lines is dependent on culture conditions, particularly on serum component; that there is a heterogeneity of ML-1 and HL-60 cells in response to the growth factors; and that there is potential importance of demonstration of heterogeneity among different cell lines in establishing requirements for different stages of differentiation.

Topics: Agar; Cell Division; Cell Line; Clone Cells; Colony-Stimulating Factors; Culture Media; Humans; Leukemia, Myeloid, Acute; Lymphocyte Activation; Lymphocytes; Phytohemagglutinins

A clonal growth of leukemic cells from the bone marrow of a patient with acute myeloid leukemia was observed in vitro for more than 20 months. Cytochemical and electron microscopic studies of the cells growing in vitro demonstrated that they were blast cells, differentiated granulocytes, and macrophages. They showed complete dependence on granulocyte-macrophage colony-stimulating factor for colony formation in agar. In addition to the presence of granulocytic colonies, some showed granulocyte-macrophage characteristics, suggesting that bipotential cells were also involved in long-term growth. Initially, they showed localized proliferation on or around giant fibroblast-like cells. Even after constant growth was established, attempts to transfer these cells were unsuccessful, and their growth was confined to the original flasks. These observations seen to indicate that their growth was not autonomous but dependent on the adherent cells in the flasks. This was also supported by a coculture experiment in which the cells were demonstrated to proliferate for 4 months only in the presence of normal bone marrow particles and bone marrow particle-derived feeder layers. These results suggest that, in some cases, long-term growth of leukemic cells can be induced in vitro by the cocultivation of bone marrow stromal cells.

Topics: Adult; Agar; Cell Differentiation; Cell Division; Chromosomes, Human; Colony-Forming Units Assay; Female; Granulocytes; Hematopoietic Stem Cells; Humans; Karyotyping; Leukemia, Myeloid, Acute; Macrophages

Topics: Agar; Colony-Forming Units Assay; Culture Media; Granulocytes; Hematopoietic Stem Cells; Humans; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute

The expression of results obtained in in vitro bone marrow cell culture as per ml of aspirated marrow instead of per 10(5) nucleated cells plated as studied in 41 patients with acute myeloid leukemia (AML) and 79 patients with a dysmyelopoietic syndrome. Four types of growth were found in AML patients: three with hypercellular marrow and either (a) microclusters, (b) isolated cells, or (c) macroclusters and a complete remission (CR) rate of 86%, 63%, and 14% respectively; (d) relative hypocellular marrow with a CR rate of 0%. This type of poorly responsive AML patient has recently been described [17]. Patients with dysmyelopoietic syndromes were subdivided into three groups according to th type of in vitro growth: subnormal, decreased, and leukemic. There was a good correlation between these in vitro groups and the outcome of patients: long survival, death from hemorrhagic and/or infectious complications, leukemic transformation (logrank test: chi 2 = 9.12, df = 2, p = 0.01).

Topics: Adult; Agar; Aged; Bone Marrow; Cell Count; Cells, Cultured; Humans; Leukemia, Myeloid, Acute; Middle Aged; Preleukemia; Prognosis

The effects of peripheral blood adherent cells from normal donors on human myeloid leukemic cluster growth in agar were studied. A prior co-incubation of nonadherent leukemic cells with adherent cell monolayers from 9 out of 10 donors in liquid cultures over a 4-hr period was sufficient to reduce subsequent leukemic growth in semisolid agar cultures. Inhibition was seen with adherent to leukemic cell ratios of as low as 0.5:1. Conversely, identical numbers of adherent cells in agar cultures but separated from the leukemic cells enhanced growth more than the cultures containing human placental conditioned media alone. Because leukemic cell exposure to adherent cells was brief, a cytotoxic mechanism appeared likely; however, this could not be detected by 51Cr release. Human peripheral blood adherent cells not activated by any in vitro mechanism suppress clonal growth of human myeloid leukemic cells by a mechanism requiring cell to cell contact. Examination of the inhibition of clonal growth appears to be more sensitive than 51Cr release as an indicator of adherent cell effects on myeloid leukemia.

Topics: Agar; Cell Adhesion; Cell Communication; Clone Cells; Culture Media; Cytotoxicity, Immunologic; Growth; Humans; Indomethacin; Leukemia, Myeloid, Acute; Time Factors

A comparison was made between the agar and methylcellulose culture systems with respect to their ability to support the clonal growth of leukemic cells obtained from patients with acute myeloblastic leukemia, acute lymphoblastic leukemia, and chronic myelogenous leukemia in blastic crisis. The number of clusters and/or colonies formed and the morphology of the cells within them varied from patient to patient. Nevertheless, no significant difference between the two culture systems within given leukemic specimens was found. No significant differences were noted among three different conditioned media used as sources of colony-stimulating activity. Most of the cells within clusters and colonies were identified to be immature members of granulocyte-macrophage series or to be indistinguishable from the preculture leukemic blast cells by morphological and cell surface marker studies. Cells from myeloid crisis in chronic myelogenous leukemia grew well in the cultures, but cells from lymphoid crisis did not proliferate.

Topics: Adult; Agar; Aged; Antigens, Surface; Cell Division; Cells, Cultured; Female; Growth Substances; Humans; Leukemia; Leukemia, Myeloid, Acute; Male; Methylcellulose; Middle Aged; Rosette Formation

Human leukaemic cell specimens were obtained from patients and directly plated into soft agar (t = 0) or cultured for 1 week in liquid phase and then plated in soft agar. Growth for 1 week in liquid phase allowed the clonal growth in agar of leukaemic specimens which were unable to clone at t = 0. Clonal growth after liquid culture consisted of the usual leukaemic type of cluster-colonies, growth of a new type of 'syncytial' cell colony or a mixture of colony types. In addition, marrow from a patient with acute lymphocytic leukaemia produced normal-appearing colonies after 1 week of growth in liquid phase. These studies suggest a similarity in the growth requirements of some leukaemic cells and normal CFUd cells.

Topics: Adult; Agar; Aged; Cell Division; Cells, Cultured; Clone Cells; Culture Media; Female; Humans; Kinetics; Leukemia; Leukemia, Erythroblastic, Acute; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Macrophages; Male; Middle Aged

Topics: Adolescent; Adult; Agar; Bone Marrow; Cell Aggregation; Cells, Cultured; Colony-Forming Units Assay; Culture Media; Female; Hematopoietic Stem Cells; Humans; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Male; Middle Aged; Prognosis

The overall reproducibility of two methods of studying granulopoiesis has been investigated, i.e. study of the granulocytic progenitor cells by the agar-culture technique and study of the myeloblast labelling index by autoradiography. The results obtained indicate that these two methods are reproducible for each step involved, from the time of marrow sampling right through to the final interpretation of the results.

Topics: Agar; Autoradiography; Cells, Cultured; Colony-Forming Units Assay; Granulocytes; Hematopoiesis; Humans; Leukemia, Myeloid, Acute; Thymidine

Topics: Agar; Anemia, Aplastic; Bone Marrow; Cells, Cultured; Colony-Forming Units Assay; Cytological Techniques; Granulocytes; Hematopoiesis; Humans; Leukemia, Lymphoid; Leukemia, Myeloid, Acute

In 8 children with acute myelomonocytic leukemia (AMML), colony formation in soft agar cultures derived from bone marrow cells was studied in an attempt to differentiate the monocytic (Schilling) from the myelomonocytic (Naegeli) types. The children did not differ markedly in their clinical and morphological parameters. Three in vitro growth patterns were observed: markedly decreased or no growth in 4 cases, extensive growth of granulocytic colonies in 2 cases, and extensive growth of macrophage colonies in the remaining 2. It is suggested that the marrows presenting diminished or no growth patterns are presumably of acute myelogenous leukemia patients with a monocytic component. The excessive granulocytic or macrophage colony growth may be an in vitro indication for an in vivo proliferation of either granulocytic or monocytic leukemic cell lines, and therefore may represent the Naegeli or Schilling variants of AMML respectively. If these observations can be approved in a larger series of AMML patients, this approach can be valuable as another tool in the differential diagnosis of the subtypes of AMML in children.

Topics: Adolescent; Agar; Bone Marrow Cells; Cells, Cultured; Child; Child, Preschool; Colony-Forming Units Assay; Female; Granulocytes; Humans; Infant; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute; Macrophages; Male; Monocytes

Bone marrow and/or blood cells from 81 patients with acute leukaemia, including 50 patients with acute myeloid leukaemia, were cultured in vitro using the agar culture method. The cells were cultured either in a single layer assay or with a normal source of colony stimulating factor (CSF) included in the culture. In almost all cases an abnormal growth pattern was seen, ranging between no growth and an excessive number of clusters. Immature granulocytic cells and macrophages were the dominating cell types in clusters and colonies from 14 out of 16 patients with acute myeloid leukaemia. In the patients with acute myeloid leukaemia the remission induction rate was found to be related to the cluster incidence. Thus, 75% of the patients with a cluster incidence between 0-100 obtained a complete remission (CR), while only 31% of the patients with a high cluster incidence (greater than 1000/2 x 10(5) cells) obtained a remission. Cluster formation in the unstimulated cultures of CSF-sensitive cells did not seem to be of prognostic value. The correlation between prognosis and in vitro growth in patients with acute myeloid leukaemia stresses the need of obtaining more insight in those factors other than drug sensitivity, which are of importance for the therapeutic response.

Topics: Adolescent; Adult; Agar; Aged; Blood Platelets; Bone Marrow; Bone Marrow Cells; Cells, Cultured; Child; Child, Preschool; Colony-Stimulating Factors; Female; Granulocytes; Hematocrit; Humans; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Leukocyte Count; Leukocytes; Macrophages; Male; Middle Aged; Prognosis; Remission, Spontaneous

The use of in-vitro culture methods for studying human haemopoietic cells has advanced greatly since 1970. These methods have contributed to our understanding of the mechanisms controlling granulopoiesis though the physiological role of colony-stimulating factor needs further clarification. In leukaemia they offer an approach to the study of possible causal factors and to the characterisation of leukaemic-cell defects. Results already obtained support the concept that the bone-marrow in acute myeloid leukaemia consists of coexisting populations of normal and leukaemic cells, with a leukaemic clone predominating in relapse and normal clones regenerating in remission. For the individual patient, in-vitro methods may prove useful in assessing prognosis and in confirming the completeness of remission; the detection of early relapse may then indicate the need for changing or re-instituting therapy. Further studies may aid the classification of the "preleukaemic" states and may help in the identification of the various causes of neutropenia.

Topics: Agar; Bone Marrow; Bone Marrow Cells; Cell Division; Clone Cells; Colony-Stimulating Factors; Culture Media; Granulocytes; Hematopoiesis; Hematopoietic Stem Cells; Humans; Leukemia, Myeloid, Acute; Monocytes; Neutropenia

Topics: Agar; Bone Marrow Cells; Cell Division; Cell Transformation, Neoplastic; Cells, Cultured; Clone Cells; Humans; In Vitro Techniques; Leukemia, Monocytic, Acute; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes

Topics: Agar; Bone Marrow; Bone Marrow Cells; Cells, Cultured; Histocytochemistry; Humans; In Vitro Techniques; Leukemia; Leukemia, Myeloid, Acute; Lymphatic Diseases; Lymphocyte Activation; Plasma Cells

Topics: Adult; Agar; Bone Marrow; Bone Marrow Cells; Cell Division; Cells, Cultured; Child; Child, Preschool; Clone Cells; Humans; Infant; Leukemia, Lymphoid; Leukemia, Myeloid, Acute

Topics: Adult; Agar; Bone Marrow; Bone Marrow Cells; Cell Division; Cells, Cultured; Child; Child, Preschool; Female; Hematopoiesis; Hematopoietic Stem Cells; Hematopoietic System; Humans; In Vitro Techniques; Infant; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Male; Middle Aged

Topics: Agar; Animals; Cell Division; Centrifugation, Density Gradient; Clone Cells; Cross Reactions; Hematopoietic Stem Cells; Humans; Immune Adherence Reaction; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Monocytes; Rabbits

Topics: Agar; Cell Count; Cell Division; Centrifugation, Density Gradient; Clone Cells; Hematopoietic Stem Cells; Humans; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Lymphocyte Activation; Myeloproliferative Disorders; Thymidine; Tritium

Topics: Agar; Bone Marrow; Bone Marrow Cells; Cells, Cultured; Humans; Leukemia, Lymphoid; Leukemia, Myeloid, Acute

Topics: Acute Disease; Agar; Anemia, Sideroblastic; Bone Marrow; Bone Marrow Cells; Cell Count; Cell Division; Culture Techniques; Gels; Humans; Leukemia, Myeloid, Acute

Topics: Agar; Electrophoresis; Erythrocytes; Freezing; Gels; Hemolysis; Humans; Isoenzymes; L-Lactate Dehydrogenase; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Lymphocytes; Myeloproliferative Disorders; Primary Myelofibrosis; Staining and Labeling

Topics: Agar; Anemia; Anemia, Hypochromic; Bone Marrow; Bone Marrow Cells; Clone Cells; Culture Media; Culture Techniques; Hodgkin Disease; Humans; Leukemia, Myeloid, Acute; Leukocytes; Multiple Myeloma

Topics: Agar; Animals; Bone Marrow; Bone Marrow Cells; Cerebrospinal Fluid; Culture Techniques; Humans; Leukemia; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Mice; Retroviridae; Sepsis

Topics: Agar; Carcinoma; Electrophoresis; Gels; Half-Life; Hodgkin Disease; Humans; Isoenzymes; L-Lactate Dehydrogenase; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Lymphatic Diseases; Lymphoma; Neoplasms; Osteosclerosis; Plasmacytoma; Sarcoma; Tissue Extracts

Topics: Agar; Aspartate Aminotransferases; Densitometry; Electrophoresis; Gels; Isoenzymes; L-Lactate Dehydrogenase; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Leukocytes; Liver; NAD; Photometry