agar and Leukemia--Lymphoid

Topics: Acute Disease; Agar; Animals; B-Lymphocytes; Cells, Cultured; Chronic Disease; Colony-Forming Units Assay; Humans; Leukemia, Lymphoid; Methylcellulose; Multiple Myeloma

The technique of bone marrow cultures has been shown to be of value in childhood acute leukemia. It now appears that acute myelogenous leukemia may be due to defective maturation of normal progenitor cells. The pattern of growth of these cells has been demonstrated to be of prognostic value. In contrast, the growth of normal progenitor cells from the bone marrow cultures of children with acute lymphocytic leukemia (ALL) may be due to the few remaining normal cells. The cause of granulocytopenia in childhood ALL is still unclear.

Topics: Acute Disease; Agar; Bone Marrow; Cell Transformation, Neoplastic; Cells, Cultured; Child; Child, Preschool; Colony-Stimulating Factors; Culture Media; Granulocytes; Hematopoiesis; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Phytohemagglutinins

Acute lymphoid leukemias (ALL) represent malignant clonal expansions of lymphoid hemopoietic cells arrested at different stages of B- or T-cell maturation. We studied surface marker profiles and cloning capability of bone marrow (BM) cells from 22 adult ALL-patients at diagnosis (n = 15) or relapse (n = 7) in agar cultures under different culture conditions in order to develop a screening system for the classification of ALL and the detection of residual leukemia. Immunophenotyping of those 22 BM-samples enabled a classification in B- or T-linear ALL. Colony growth of BM-cells could be obtained in four out of 20 cases of ALL at diagnosis and in one case at relapse. Different stimulating factors and their combinations (GM-CSF; IL-1; IL-2; IL-3; IL-4; IL-6; placenta conditioned media (PCM); Phytohemagglutinin (PHA, 40%) and lipopolisaccaride (LPS, 1.25%)--containing conditioned media ('B-ly'); IL-1+IL-3; IL-1+IL-4; IL-1+IL-6; IL-1+B-ly) did not show an overall significant difference in stimulating ALL-clones. Immunological phenotyping of ALL-clones in these 5 cases could prove the lymphoid leukemic character of the clones obtained.. Our data show that colony growth of ALL-BM-cells is difficult. Nevertheless, in cases where colony growth could be obtained those clones showed the original surface marker profile of the leukemic cells proving the specificity of our colony assay.

Topics: Acute Disease; Adult; Agar; Bone Marrow Cells; Cell Culture Techniques; Clone Cells; Humans; Immunophenotyping; Leukemia, Lymphoid; Tumor Cells, Cultured

A preliminary study of the culture of leukemic progenitor cells (CFU-L) from acute non-T lymphoblastic leukemia was undertaken for 25 patients (19 were considered in complete remission and 6 in the acute phase of the disease). Two culture systems were tested; a double layer agar-liquid phase and a single layer of methylcellulose. The major problem was the characterization of the colonies: immunological labelling coupled with cytofluorometry, as well as cytomorphology, cytogenetic and more recently molecular biology may allow the characterization of the CFU-L. The culture of CFU-L appears to be an efficient method for detecting residual leukemic cells which can be used to evaluate the quality of both the remission obtained and that of autologous bone marrow after purging.

Topics: Agar; Colony-Forming Units Assay; Culture Media; Growth Substances; Humans; Leukemia, Lymphoid; Leukocytes; Lymphocyte Depletion; Methylcellulose; T-Lymphocytes; Tumor Stem Cell Assay

Topics: Agar; Cell Division; Child; Child, Preschool; Hematopoiesis; Hematopoietic Stem Cells; Humans; In Vitro Techniques; Infant; Leukemia, Lymphoid

Topics: Agar; Colony-Forming Units Assay; Culture Media; Granulocytes; Hematopoietic Stem Cells; Humans; Leukemia, Lymphoid; Leukemia, Monocytic, Acute; Leukemia, Myeloid, Acute

Topics: Adolescent; Adult; Agar; Bone Marrow; Cell Aggregation; Cells, Cultured; Colony-Forming Units Assay; Culture Media; Female; Hematopoietic Stem Cells; Humans; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Male; Middle Aged; Prognosis

Peripheral blood lymphocytes from normal donors and patients with chronic lymphocytic leukemia, B-cell type, were purified into T, helper T, and suppressor T lymphocytes by fluorescence-activated cell sorting using OKT3, OKT4, and OKT8 monoclonal antibodies. The maximum response of the purified subpopulations to stimulation by phytohemagglutinin (PHA) was determined by measuring the production of colonies when the stimulated cells were grown on agar. The helper T cells in normal and CLL patients were the most responsive to PHA stimulation, although the responsiveness of helper T cells to PHA was decreased in CLL. Purified CLL B cells responded minimally to PHA stimulation, but normal B lymphocytes did not. The abnormal response of CLL lymphocytes to PHA appears to be due abnormal helper T cells, and, to a smaller extent, to the ability of CLL B lymphocytes to respond.

Topics: Agar; Aged; Antibodies, Monoclonal; Cell Membrane; Clone Cells; Humans; Leukemia, Lymphoid; Leukocyte Count; Lymphocyte Activation; Lymphocytes; Male; Middle Aged; Phenotype; Phytohemagglutinins; T-Lymphocytes

Topics: Agar; Anemia, Aplastic; Bone Marrow; Cells, Cultured; Colony-Forming Units Assay; Cytological Techniques; Granulocytes; Hematopoiesis; Humans; Leukemia, Lymphoid; Leukemia, Myeloid, Acute

Bone marrow and/or blood cells from 81 patients with acute leukaemia, including 50 patients with acute myeloid leukaemia, were cultured in vitro using the agar culture method. The cells were cultured either in a single layer assay or with a normal source of colony stimulating factor (CSF) included in the culture. In almost all cases an abnormal growth pattern was seen, ranging between no growth and an excessive number of clusters. Immature granulocytic cells and macrophages were the dominating cell types in clusters and colonies from 14 out of 16 patients with acute myeloid leukaemia. In the patients with acute myeloid leukaemia the remission induction rate was found to be related to the cluster incidence. Thus, 75% of the patients with a cluster incidence between 0-100 obtained a complete remission (CR), while only 31% of the patients with a high cluster incidence (greater than 1000/2 x 10(5) cells) obtained a remission. Cluster formation in the unstimulated cultures of CSF-sensitive cells did not seem to be of prognostic value. The correlation between prognosis and in vitro growth in patients with acute myeloid leukaemia stresses the need of obtaining more insight in those factors other than drug sensitivity, which are of importance for the therapeutic response.

Topics: Adolescent; Adult; Agar; Aged; Blood Platelets; Bone Marrow; Bone Marrow Cells; Cells, Cultured; Child; Child, Preschool; Colony-Stimulating Factors; Female; Granulocytes; Hematocrit; Humans; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Leukocyte Count; Leukocytes; Macrophages; Male; Middle Aged; Prognosis; Remission, Spontaneous

Topics: Agar; Animals; Cell Line; Cell Separation; Cell Survival; Cytarabine; Deoxycytidine; DNA, Neoplasm; Leucine; Leukemia, Lymphoid; Mesylates; Mice; Mutagens; Mutation; Neoplasm Proteins; RNA, Neoplasm; Temperature; Tritium; Uridine

Topics: Adult; Agar; Bone Marrow; Bone Marrow Cells; Cell Division; Cells, Cultured; Child; Child, Preschool; Clone Cells; Humans; Infant; Leukemia, Lymphoid; Leukemia, Myeloid, Acute

Topics: Adult; Agar; Bone Marrow; Bone Marrow Cells; Cell Division; Cells, Cultured; Child; Child, Preschool; Female; Hematopoiesis; Hematopoietic Stem Cells; Hematopoietic System; Humans; In Vitro Techniques; Infant; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Male; Middle Aged

Topics: Agar; B-Lymphocytes; Burkitt Lymphoma; Cell Division; Cell Line; Clone Cells; Culture Media; Humans; Immune Adherence Reaction; Leukemia, Lymphoid; T-Lymphocytes

Topics: Agar; Animals; Avian Sarcoma Viruses; Cell Line; Cell Transformation, Neoplastic; Clone Cells; Cricetinae; Cytological Techniques; Female; Fibroblasts; HeLa Cells; Humans; Kidney; L Cells; Leukemia, Lymphoid; Lung; Mammary Neoplasms, Experimental; Methods; Mice; Mice, Inbred C3H; Mice, Inbred DBA; Ovary; Polyomavirus; Rats; Sarcoma, Yoshida

Topics: Agar; Bone Marrow; Bone Marrow Cells; Cells, Cultured; Humans; Leukemia, Lymphoid; Leukemia, Myeloid, Acute

Topics: Adolescent; Adult; Agar; Aging; Blood Proteins; Child; Child, Preschool; Electrophoresis; Gels; Humans; Hydrogen-Ion Concentration; Infant; Infant, Newborn; Leukemia, Lymphoid; Lymph Nodes; Lymphocytes; Middle Aged; Organ Specificity; Palatine Tonsil; Photomicrography; Proteins; Solubility; Starch; Thymus Gland

Topics: Agar; Aspartate Aminotransferases; Densitometry; Electrophoresis; Gels; Isoenzymes; L-Lactate Dehydrogenase; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Leukocytes; Liver; NAD; Photometry

Topics: Agar; Antimetabolites; Bacteriological Techniques; Blood Transfusion; Bone Marrow; Bone Marrow Diseases; Humans; Leukemia; Leukemia, Lymphoid; Mycoplasma; Penicillins; Radiotherapy