agar and Leukemia--Erythroblastic--Acute

Human leukaemic cell specimens were obtained from patients and directly plated into soft agar (t = 0) or cultured for 1 week in liquid phase and then plated in soft agar. Growth for 1 week in liquid phase allowed the clonal growth in agar of leukaemic specimens which were unable to clone at t = 0. Clonal growth after liquid culture consisted of the usual leukaemic type of cluster-colonies, growth of a new type of 'syncytial' cell colony or a mixture of colony types. In addition, marrow from a patient with acute lymphocytic leukaemia produced normal-appearing colonies after 1 week of growth in liquid phase. These studies suggest a similarity in the growth requirements of some leukaemic cells and normal CFUd cells.

Topics: Adult; Agar; Aged; Cell Division; Cells, Cultured; Clone Cells; Culture Media; Female; Humans; Kinetics; Leukemia; Leukemia, Erythroblastic, Acute; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Macrophages; Male; Middle Aged

Topics: Agar; Animals; Autoradiography; Cattle; Cell Differentiation; Cell Division; Clone Cells; Culture Media; Culture Techniques; Dimethyl Sulfoxide; DNA; Leukemia, Erythroblastic, Acute; Mice; Rats; Thymidine