agar and Leishmaniasis--Visceral

Serial dilution of blood and spleen biopsy specimens, plated on Novy-MacNeal-Nicolle (NNN) blood agar using microtiter culture plates, is a sensitive and reproducible method for detection and growth of Leishmania parasites. Plates could be easily monitored, and growth could be rapidly detected. Moreover, parasite number may be estimated using this technique.

Topics: Agar; Blood; Culture Media; Humans; Leishmania; Leishmaniasis, Visceral; Reproducibility of Results; Sensitivity and Specificity; Spleen

A simple dual culture agar plating technique has been developed and evaluated for its efficiency in determining the relationship of gut bacteria of sandfly with Leishmania donovani promastigotes. There are about twenty morphologically distinct bacterial colonies have been isolated from the gut homogenate of Phlebotomus argentipes. In dual culture method, each bacterial isolate was inoculated in one half of the plate and the promastigotes of Leishmania was inculcated in the other half by streaking. After incubation, the type of association was determined based on the presence or absence of promastigotes colonies. The reliability of this method was compared with broth dilution method in 96 well plate.

Topics: Agar; Animals; Bacteria; Blood; Culture Media; Gastrointestinal Tract; Host-Parasite Interactions; Humans; Insect Vectors; Leishmania donovani; Leishmaniasis, Visceral; Phlebotomus

Topics: Agar; Animals; Bacteria; Culture Media; Leishmania; Leishmaniasis, Visceral; Yeasts

Topics: Agar; Culture Media; Humans; Kenya; Leishmania donovani; Leishmaniasis, Visceral; Spleen

Topics: Agar; Animals; Blood; Cattle; Culture Media; Evaluation Studies as Topic; Humans; India; Leishmania; Leishmaniasis, Visceral; Rabbits