agar and Intestinal-Diseases

To compare three different chromogenic agars and MacConkey agar for the detection of aerobic Gram-negative bacteria in the normal intestinal microflora and to assess the accuracy of the chromogenic agars for the direct identification of Escherichia coli.. A total of 164 Gram-negative clinical isolates (E. coli, Proteus, Klebsiella, Enterobacter, Morganella and Pseudomonas species) and 30 stool specimens were inoculated in parallel on four media: Chromagar E. coli/Coliform, Chromogenic urinary tract infection UTI medium, CHROMagar Orientation and MacConkey agar. All colonies that differed by color and/or morphology were selected for further identification by VITEK 1 and/or API 20E from each medium.. On E. coli/Coliform agar five out of 32 (16%) E. coli strains failed to produce the color as described by the manufacturer. No remarkable discrepancies were found for the other clinical isolates. There was no significant difference in detection rate (DR) of aerobic Gram-negative bacteria in stool specimens between the different chromogenic agars and MacConkey agar. The overall DR was about 84%, and varied from 100% for monomicrobial specimens to 33% for polymicrobial specimens. The positive predictive values (PPV) for the direct identification of E. coli on Chromagar E. coli/Coliform, Chromogenic UTI medium and CHROMagar Orientation were 1.00, 0.93 and 0.93, respectively. The negative predictive values (NPV) were 0.53, 0.68 and 0.69, respectively.. Chromogenic UTI medium and CHROMagar Orientation are the preferred media because of the higher NPV. The high PPV of these agars allows accurate and rapid identification of E. coli.

Topics: Agar; Chromogenic Compounds; Culture Media; Escherichia coli; Escherichia coli Infections; Feces; Gram-Negative Bacteria; Humans; Intestinal Diseases; Predictive Value of Tests

An examination of 320 random stool samples from patients aged 7-60 years in Kafr-Sakr Hospital, was done to compare the efficacy of modified agar plate method to traditional method for diagnosis of Strongyloides stercoralis infection. Out of 320 cases examined 27 were positive for strongyloidiasis by all means. The traditional methods detected 37% and 48.1% from the positive cases by single and repeated 3 days examination respectively. The modified agar plate detected 70.3% and 96.2% among positive cases by single and 3 days examination respectively. The modified agar plate was twice efficient than the traditional methods. Precautions taken to obtain the best result were discussed. Considering the other advantages of this technique, it was concluded that it may eventually become the test of choice in diagnosis of strongyloidiasis.

Topics: Adolescent; Adult; Agar; Animals; Child; Feces; Female; Humans; Intestinal Diseases; Male; Middle Aged; Strongyloides stercoralis; Strongyloidiasis

(1) To evaluate the performance of in-house and pre-poured commercially available enteric agar by challenge with a large number of positive clinical specimens. (2) To set the standard (critical independent evaluation) which new products should reach. (3) To publish this information, so that others can make informed decisions about enteric media.. Thirteen media of anonymous source were challenged with "known" positive stool samples.. In-house desoxycholate citrate agar performed best for overall pathogen isolation rates, for shigella isolation rates, and for most pathogens available on primary culture.. Desoxycholate citrate agar made by our own laboratory yielded the most pathogens and proved the most effective.

Topics: Agar; Citrates; Culture Media; Deoxycholic Acid; Feces; Humans; Intestinal Diseases; Microbiological Techniques; Quality Control; Reference Standards

Topics: Agar; Biopsy; Humans; Intestinal Diseases; Intestinal Mucosa; Paraffin

Topics: Adult; Agar; Antigens; Bacteriophage Typing; Bile Acids and Salts; Child; Child, Preschool; Culture Media; Czechoslovakia; Escherichia coli; Escherichia coli Infections; Feces; Female; Humans; Intestinal Diseases; Male; Serotyping; Shigella

Topics: Agar; Bile; Culture Media; Disease; Glycerol; Intestinal Diseases; Intestines; Salmonella; Shigella

Topics: Agar; Aged; Culture Media; Disease; Employment; Humans; Intestinal Diseases; Intestines