agar and Infertility

The current study aimed to determine the causes associated with ocular infection in cats received at Baghdad veterinary hospital from March 2020 to April 2021. Forty cats (22 females and 18 males) were examined at a small animal clinic in Baghdad veterinary hospital from March 2020 to April 2021. The cats suffered from severe eyes infection (inflammation, lacrimation, redness and other ocular signs). On the other hand, ten healthy cats were examined and prepared for bacterial isolation as a control group. For bacterial isolation, sterile cotton swabs with transport medium were taken gently from the corneal and conjunctiva area of infected eyes. The swabs were placed in an ice box within 24 hours for laboratory culture. Sterile swabs with transport media were used in our study; swabs passed directly on the inferior conjunctival sac of the compromised eye avoiding contact with eyelashes and skin of eyelids. All swabs were inoculated on the following media (5% Sheep blood agar, MacConkey agar and Nutrient agar) at 37ºC for 24 to 48 h.ImmunoChromatoGraphy assay (ICG) of FCV on samples. The results showed that 50%of Mixed bacterial and FCV were the significant cause of isolates; also, it showed that

Topics: Agar; Animals; Cat Diseases; Cats; Culture Media; Eye Infections; Female; Infertility; Male; Sheep; Sheep Diseases; Staphylococcus aureus

Zona pellucida (ZP) abnormalities are the cause of low fertility or infertility, agar-like ZP is more common in abnormal ZP. The purpose of this exploration is to systematically analyze the fertilization competence of agar-like ZP oocytes, the development characteristics of subsequent embryos as well as the results of embryo transfer, aiming to explore effective clinical treatment strategies. A total of 58 patients with agar-like ZP were set as the case group and the control group involved 3866 patients, in which the patients' oocytes presented normal ZP. BMI, basal hormone levels, and hormone levels were similar in both groups. The case patients suffered significantly longer infertility years than control (p<0.05), and most patients were diagnosed with pelvic inflammatory diseases. A distinct difference was observed in the structure of oocyte corona cumulus complexes between the two groups. The embryo development parameters, which include the rates of cleavage, high-quality embryo, blastocyst, and high-quality blastocyst in the case group were greatly lower than that in the control group (p<0.05). The rates of cumulative clinical pregnancy and live birth were comparable between the two groups. In the subsequent follow-up, thirty-four of the 58 patients receiving intracytoplasmic single sperm injection (ICSI) or early rescue ICSI (R-ICSI) treatment successfully gave birth to babies, and all of the newborns were with no neonatal defects. In addition, the fertilization rate of the R-ICSI group was significantly lower than that of the ICSI group (p<0.05). The occurrence of agar-like ZP impairs the development competence of human oocytes, however, the human oocytes with agar-like ZP can develop into healthy offspring, and an ICSI regimen is the optimal treatment strategy for them.

Topics: Agar; Female; Hormones; Humans; Infant, Newborn; Infertility; Male; Oocytes; Pregnancy; Semen; Zona Pellucida

Topics: Agar; Electrophoresis; Escherichia coli; Female; In Vitro Techniques; Infertility; Male; Serum Albumin

Topics: Agar; Anti-Infective Agents, Local; Biological Assay; Biological Products; Candida; Infection Control; Infertility; Mercury Compounds; Mycoses; Research; Staphylococcus; Thimerosal

Topics: Agar; Bacteriology; Humans; Infertility