agar and Infectious-Mononucleosis

Topics: Agar; Animals; Antigens; Burkitt Lymphoma; Centrifugation; Culture Techniques; Herpesviridae; Herpesviridae Infections; Herpesvirus 4, Human; Humans; Infectious Mononucleosis; Microscopy, Electron; Oncogenic Viruses; Serologic Tests

Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA)-positive lymphoblastoid cells grew as colonies in soft agar after seeding of leukocytes from the peripheral blood of four patients with infectious mononucleosis serologically determined to be caused by EBV. In individual cases more colonies were obtained from blood specimens during the acute phase of the disease than during the convalescent phase. Incorporation of human umbilical cord serum, which contained neutralizing antibody to EBV, into the agar medium did not reduce the number of colonies developing. Our observations indicate that colony-forming cells were originally present in the blood samples, and that they were not infected and subsequently transformed in vitro. Cells from less than 20% of the EBNA-positive colonies grew to form lymphoblastoid cell lines, which were EBNA-positive and had B lymphocyte surface markers. However, the majority (over 80%) of the EBNA-positive colonies failed to form immortalized cell lines. No colonies were obtained from 91 blood samples from healthy young adults and from five patients with an IM-like disease unrelated to EBV infections. The present results strongly suggest that already transformed cells or cells very easily transformed by EBV are present in the blood of IM patients.

Topics: Adult; Agar; Antibodies, Viral; Antigens, Viral; Cell Division; Cell Line; Cell Nucleus; Child, Preschool; Clone Cells; Epitopes; Female; Herpesvirus 4, Human; Humans; Infectious Mononucleosis; Leukocytes; Male

It was shown by the modified method of agar cultures that in the peripheral blood of a healthy man, aged from 4 days to 40 years. The number of cell precursors of granulocytes and macrophages (CFU-C) varied from 0.05 to 6.38 in adults and from 0.2 to 2.9 in children per 105 nuclear cells. CFU-C content in the patients with infectious mononucleosis and acute leukemia was 0.5--14 and 0--0.3 per 105 nuclear cells, respectively.

Topics: Acute Disease; Adolescent; Adult; Agar; Cell Division; Child; Child, Preschool; Clone Cells; Female; Gels; Humans; Infant; Infant, Newborn; Infectious Mononucleosis; Leukemia; Leukocytes; Male; Middle Aged

Topics: Agar; Agglutination Tests; Animals; Antigen-Antibody Reactions; Cattle; Cell Membrane; Complement System Proteins; Cricetinae; Erythrocytes; Forssman Antigen; Graft Rejection; Guinea Pigs; Hemadsorption; Hemolysis; Histocompatibility Antigens; Humans; Immune Sera; Immunodiffusion; Immunoglobulin G; Immunoglobulin M; Infectious Mononucleosis; Mice; Rabbits; Rats; Sheep; Species Specificity; Thymus Gland

Topics: Agar; Animals; Antibodies; Complement System Proteins; Hemolysin Proteins; Hemolysis; Immunodiffusion; Immunoelectrophoresis; In Vitro Techniques; Infectious Mononucleosis; Sheep