agar and Gastroenteritis

Oral rehydration is the main treatment of acute diarrhea in children. This study was undertaken to evaluate the efficacy and safety of xyloglucan and gelose (agar-agar) plus oral rehydration solution (ORS) compared with placebo and ORS for reduction of acute diarrhea symptoms in children.. In a randomized, double-blind, placebo-controlled trial, children with acute gastroenteritis received xyloglucan/gelose plus ORS (n = 50) or placebo plus ORS (n = 50) for 5 days. Demographic, clinical, anthropometric and laboratory parameters were recorded and analyzed.. Xyloglucan/gelose plus ORS was effective and safe in treating acute diarrhea in children.

Topics: Acute Disease; Adolescent; Agar; Antidiarrheals; Child; Child, Preschool; Diarrhea; Double-Blind Method; Female; Fluid Therapy; Gastroenteritis; Glucans; Humans; Infant; Male; Prospective Studies; Rehydration Solutions; Treatment Outcome; Xylans

This research aims to compare the culturing conditions for enterohepatic Helicobacter, evaluating culture media, incubation atmosphere and susceptibility to antimicrobials used to generate selective conditions. Four common media for the closely related genus Campylobacter (Columbia, Bolton, Brucella and CCDA agar), as well as the need for hydrogen in the microaerobic incubation atmosphere, were evaluated. Serial dilutions of 13 strains belonging to six species (H. apodemus, H. bilis, H. canicola, H. canis, H. equorum and Helicobacter sp.) were inoculated in each media and incubated at 37°C for 48 to 96 h using CampyGen (OXOID) and gaseous exchange (including hydrogen) in parallel. Columbia or Brucella agars were the most appropriate for culturing EHH (P < 0·05). However, there was no significant difference between the atmospheres evaluated (P = 0·13). In addition, minimal inhibitory concentration for six antibiotics showed that all isolates were resistant to trimethoprim, whereas for the rest of the antibiotics (cephalothin, cefoperazone, cefsulodin, teicoplanin and vancomycin) the inhibition range was between 8 and 64 μg ml

Topics: Agar; Anti-Bacterial Agents; Campylobacter; Culture Media; Gastroenteritis; Helicobacter; Helicobacter Infections; Humans; Microbial Sensitivity Tests

Acute gastroenteritis (AGE) is one of the most common diseases in children, particularly in those under the age of 5 years in whom a severe picture of hydroelectrolyte imbalance may be triggered accompanied, in most cases, by leukocyte response. The aim of this study was to evaluate three new culture media for the isolation of enteropathogens in infantile diarrhea.. From April to September, 1993, 170 samples of diarrhea stools from children up to 7 years of age attending pediatric hospitals in Bogota were studied. Three new culture media were used in the isolation of the enteropathogenic bacteria: BGLN, MILA agar, Rambach agar and a conventional agar medium S.S. Biochemical tests were performed to identify the isolated bacteria.. A total of 98.5% of enteropathogenic E. coli was isolated in Rambach agar, which also presented excellent recovery rates of Salmonella sp. (100%) versus S.S> (64.3%) and BGLN (77.2%), respectively. The best selectivity for Shigella sp. was observed with BGLN with a 100% recovery rate. Out of the 170 samples 105 showed a leukocyte count of 70-75% and positive isolation for enteropathogenic bacteria. Six samples with the same leukocyte count did not present enteropathogenic bacteria, with the 59 remaining samples with a 20-25% PMN count being negative for enteropathogenic bacteria.. The results suggest that the new culture media used in this study may have better recovery rates for enteropathogenic bacteria in acute gastroenteritis. Likewise, a correlation was observed between leukocyte count and isolation enteropathogenic bacteria.

Topics: Acute Disease; Agar; Child; Child, Preschool; Escherichia coli; Evaluation Studies as Topic; Gastroenteritis; Glycerol; Humans; Infant; Infant, Newborn; Iron; Lactose; Lysine; Novobiocin; Salmonella; Shigella

Campylobacter upsaliensis is the name which has been proposed for a new group of thermophilic campylobacter strains which differ from C. jejuni and C. coli in having a negative or weak catalase reaction. Primary isolation of these strains from human feces has been achieved only by use of filtration techniques. We report here direct isolation of strains corresponding to C. upsaliensis from stools of six children. The strains were isolated on a newly described campylobacter-selective medium. The strains were oxidase positive, hippurate negative, nitrate positive, negative for H2S in triple sugar iron, and susceptible to cephalothin (30-micrograms disk) and nalidixic acid (30-micrograms disk), and they grew at 37 and 43 degrees C, but not at 25 degrees C. The selective medium used was a blood-free, charcoal-based medium consisting of Columbia agar base, activated charcoal, cefoperazone (32 micrograms/ml), vancomycin (20 micrograms/ml), and cycloheximide (100 micrograms/ml). The medium supported the growth of the weakly reacting or catalase-negative strains, with colony counts equivalent to those obtained on antibiotic-free horse blood agar. These strains could not be isolated directly from stool on Skirrow medium, and colony counts confirmed that this medium could not support a low inoculum of these organisms. The clinical significance of these strains is unknown. We conclude that C. upsaliensis can be isolated directly from stool by using a selective medium, without the need for filtration.

Topics: Agar; Campylobacter; Campylobacter Infections; Child; Feces; Gastroenteritis; Humans; Temperature

The simultaneous recovery of Aeromonas hydrophila and Aeromonas caviae from the stool of a 49-year-old woman with watery diarrhea was facilitated through the use of a blood agar medium which detected the hemolytic capability of A. hydrophila. In vitro phenotypic tests support the conclusion that only the A. hydrophila isolate was clinically significant.

Topics: Aeromonas; Agar; Blood; Feces; Female; Gastroenteritis; Humans; Middle Aged

Topics: Adolescent; Adult; Agar; Aged; Animals; Anti-Bacterial Agents; Bacteriological Techniques; Bile; Caniformia; Carrier State; Child; Child, Preschool; Citrates; Disease Reservoirs; Drug Resistance, Microbial; Enterobacteriaceae; Enterobacteriaceae Infections; Feces; Female; Gastroenteritis; Humans; Indoles; Infant; Male; Middle Aged; Rectum; Reptiles; Sepsis; Thailand; Zoonoses

Topics: Agar; Amylases; Bacteria; Carrageenan; Child; Chromatography, Thin Layer; Food Analysis; Galactose; Galactosemias; Gastroenteritis; Glycoside Hydrolases; Humans; Resins, Plant; Suspensions