agar and Chronic-Disease

agar has been researched along with Chronic-Disease* in 21 studies

Reviews

1 review(s) available for agar and Chronic-Disease

ArticleYear
Human B cell colony assays.
    Blut, 1987, Volume: 55, Issue:4

    Topics: Acute Disease; Agar; Animals; B-Lymphocytes; Cells, Cultured; Chronic Disease; Colony-Forming Units Assay; Humans; Leukemia, Lymphoid; Methylcellulose; Multiple Myeloma

1987

Other Studies

20 other study(ies) available for agar and Chronic-Disease

ArticleYear
Assessing Pseudomonas aeruginosa virulence and the host response using murine models of acute and chronic lung infection.
    Methods in molecular biology (Clifton, N.J.), 2014, Volume: 1149

    Murine models of acute and chronic lung infection have been used in studying Pseudomonas aeruginosa for assessing in vivo behavior and for monitoring of the host response. These models provide an important resource for studies of the initiation and maintenance of bacterial infection, identify bacterial genes essential for in vivo maintenance and for the development and testing of new therapies. The rat has been used extensively as a model of chronic lung infection, whereas the mouse has been a model of acute and chronic infection. Intratracheal administration of planktonic bacterial cells in the mouse provides a model of acute pneumonia. Bacteria enmeshed in agar beads can be used in the rat and mouse to reproduce the lung pathology of cystic fibrosis patients with advanced chronic pulmonary disease. Here, we describe the methods to assess virulence of P. aeruginosa using prototype and clinical strains in the Sprague-Dawley rat and the C57BL/6NCrlBR mouse by monitoring several measurable read-outs including weight loss, mortality, in vivo growth curves, the competitive index of infectivity, and the inflammatory response.

    Topics: Acute Disease; Agar; Animals; Biological Assay; Chronic Disease; Colony Count, Microbial; Disease Models, Animal; Host-Pathogen Interactions; Inflammation; Kinetics; Lung; Male; Mice, Inbred C57BL; Pseudomonas aeruginosa; Pseudomonas Infections; Rats, Sprague-Dawley; Respiratory Tract Infections; Survival Analysis; Virulence

2014
Improved outcome of chronic Pseudomonas aeruginosa lung infection is associated with induction of a Th1-dominated cytokine response.
    Clinical and experimental immunology, 2002, Volume: 127, Issue:2

    Repeated challenge with antigen is involved in the pathogenesis of a variety of pulmonary diseases. Patients with cystic fibrosis (CF) experience recurrent pulmonary colonization with Pseudomonas aeruginosa before establishment of chronic lung infection. To mimic recurrent lung infections in CF patients, the lungs of susceptible BALB/c mice were re-infected with P. aeruginosa 14 days after the initial infection. Singly-infected BALB/c mice, as well as non-infected mice, were used as controls. Decreased mortality and milder lung inflammation in re-infected BALB/c mice, as well as a tendency for improved clearance of bacteria, was observed when compared with singly-infected mice. The improved outcome in re-infected mice correlated with changes in CD4 cell numbers. Surface expression of LFA-1 on pulmonary CD4 cells was increased in re-infected compared with singly-infected mice. Moreover, resistance to re-infection was paralleled by a shift towards a Th1-dominated response and increased IL-12 production. No significant increase in serum IgG was observed in the re-infected mice. In conclusion, these results indicate a protective role for a Th1-dominated response, independent of antibody production, in chronic P. aeruginosa lung infection in CF.

    Topics: Agar; Alginates; Animals; Antibodies, Bacterial; Bronchoalveolar Lavage Fluid; CD4 Lymphocyte Count; Chronic Disease; Cytokines; Female; Glucuronic Acid; Hexuronic Acids; Immunoglobulin G; Interferon-gamma; Interleukin-12; Interleukin-4; Lymphocyte Function-Associated Antigen-1; Mice; Mice, Inbred BALB C; Models, Animal; Pneumonia, Bacterial; Pseudomonas aeruginosa; Pseudomonas Infections; Recurrence; Th1 Cells

2002
Effects of Chinese medicinal herbs on a rat model of chronic Pseudomonas aeruginosa lung infection.
    APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 1996, Volume: 104, Issue:5

    The aim of the study was to evaluate the effects of two kinds of Chinese medicinal herbs, Isatis tinctoria L (ITL) and Daphne giraldii Nitsche (DGN), on a rat model of chronic Pseudomonas aeruginosa lung infection mimicking cystic fibrosis (CF). Compared to the control group, both drugs were able to reduce the incidence of lung abscess (p < 0.05) and to decrease the severity of the macroscopic pathology in lungs (p < 0.05). In the great majority of the rats, the herbs altered the inflammatory response in the lungs from an acute type inflammation, dominated by polymorphonuclear leukocytes (PMN), to a chronic type inflammation, dominated by mononuclear leukocytes (MN). DGN also improved the clearance of P. aeruginosa from the lungs (p < 0.03) compared with the control group. There were no significant differences between the control group and the two herbal groups with regard to serum IgG and IgA anti-P. aeruginosa sonicate antibodies. However, the IgM concentration in the ITL group was significantly lower than in the control group (p < 0.03). These results suggest that the two medicinal herbs might be helpful to CF patients with chronic P. aeruginosa lung infection, DGN being the most favorable.

    Topics: Agar; Animals; Antibodies, Bacterial; Chronic Disease; Culture Media; Cystic Fibrosis; Disease Models, Animal; Drugs, Chinese Herbal; Female; Lung Diseases; Microbial Sensitivity Tests; Pseudomonas aeruginosa; Pseudomonas Infections; Rats; Rats, Inbred Lew

1996
Heterogeneity of Borrelia burgdorferi in the skin.
    The American Journal of dermatopathology, 1996, Volume: 18, Issue:6

    The reliability of various in vitro techniques to identify Borrelia burgdorferi infection is still unsatisfactory. Using a high-power resolution videomicroscope and staining with the borrelia genus-specific monoclonal flagellar antibody H9724, we identified borrelial structures in skin biopsies of erythema chronicum migrans (from which borrelia later was cultured), of acrodermatitis chronica atrophicans, and of morphea. In addition to typical borreliae, we noted stained structures of varying shapes identical to borreliae found in a "borrelia-injected skin" model; identical to agar-embedded borreliae; and identical to cultured borreliae following exposure to hyperimmune sera and/or antibiotics. We conclude that the H9724-reactive structures represent various forms of B. burgdorferi rather than staining artifacts. These "atypical" forms of B. burgdorferi may represent in vivo morphologic variants of this bacterium.

    Topics: Acrodermatitis; Agar; Anti-Bacterial Agents; Antibodies, Monoclonal; Artifacts; Bacteriological Techniques; Borrelia burgdorferi Group; Chronic Disease; Coloring Agents; Culture Media; Culture Techniques; Erythema Chronicum Migrans; Flagella; Granuloma Annulare; Humans; Immune Sera; Immunohistochemistry; Lyme Disease; Microscopy, Video; Scleroderma, Localized; Skin

1996
[Agar migration test for studying the leukocyte migration inhibitory factor].
    Laboratornoe delo, 1981, Issue:6

    Topics: Agar; Cerebral Infarction; Chronic Disease; Humans; Leukocyte Migration-Inhibitory Factors; Lymphokines; Methods; Multiple Sclerosis; Nephritis; Tuberculosis, Pulmonary

1981
Granulopoiesis in chronic myeloproliferative disorders in children.
    Pediatrics, 1978, Volume: 61, Issue:6

    Four children with chronic myeloproliferative disorders (three with Philadelphia [Ph1] chromosome-positive chronic myelogenous leukemia [CML] were studied with soft agar culture at diagnosis (before therapy) in an attempt to define abnormalities in granulopoiesis. The three patients with CML had elevated peripheral blood golony-forming cells (CFCs) and/or normal or decreased bone marrow CFCs (in those studied). Colony-stimulating activity (CSA) was markedly decreased or absent at diagnosis in all three. Maturation of myeloid cells eithin the colonies in agar was normal, indicating that no block in myeloid maturation was present. These findings are in general agreement with results previously reported in untreated adults with Ph1 chromosome-positive CML and further define the similarity with the adult form of the disease. One Ph1 chromosome-negative patient with a clinically similar chronic myeloproliferative disorder was studied and had similarly elevated peripheral blood CFCs. She had normal CSA with a similarly high WBC count. This finding was unexpected and suggests that, unlike the patients with CML, her monocytes were capable of elaboration CSA. This difference might prove helpful in the classification of this type of disorder in cases where the Ph1 chromosome abnormality is not present.

    Topics: Adolescent; Agar; Bone Marrow; Cell Division; Child; Child, Preschool; Chromosomes, Human, 21-22 and Y; Chronic Disease; Clone Cells; Culture Media; Female; Granulocytes; Hematopoiesis; Humans; Leukemia, Myeloid; Leukocytes; Male; Myeloproliferative Disorders

1978
A second family with hemoglobin M Saskatoon in Saskatchewan.
    Clinical biochemistry, 1974, Volume: 7, Issue:2

    Topics: Adult; Agar; Amino Acid Sequence; Amino Acids; Blood Protein Electrophoresis; Chromatography, Gel; Chromatography, Ion Exchange; Chronic Disease; Cyanosis; Drug Stability; Electrophoresis; Electrophoresis, Paper; Electrophoresis, Starch Gel; Female; Hemoglobins, Abnormal; Hot Temperature; Humans; Pedigree; Peptide Fragments; Saskatchewan; Solubility; Spectrophotometry; Spectrophotometry, Ultraviolet; Trypsin

1974
Comparison of electrophoresis on agar gel and agarose gel in the evaluation of gamma-globulin abnormalities in cerebrospinal fluid and serum in multiple sclerosis.
    Clinica chimica acta; international journal of clinical chemistry, 1973, Jul-27, Volume: 46, Issue:4

    Topics: Adolescent; Adult; Agar; Brain Diseases; Child; Chronic Disease; Electrophoresis; Evaluation Studies as Topic; Female; gamma-Globulins; Humans; Male; Methods; Middle Aged; Multiple Sclerosis; Optic Neuritis; Polysaccharides

1973
[Method of analysis of isoenzymes of malate dehydrogenase in the urine].
    Laboratornoe delo, 1973, Volume: 11

    Topics: Agar; Child; Child, Preschool; Chronic Disease; Electrophoresis; Glomerulonephritis; Humans; Isoenzymes; Malate Dehydrogenase; Methods; Pyelonephritis

1973
[Data on hepatitis antigen (HAA)].
    Deutsche Zeitschrift fur Verdauungs- und Stoffwechselkrankheiten, 1972, Volume: 32, Issue:1

    Topics: Acute Disease; Adolescent; Adult; Agar; Age Factors; Child; Child, Preschool; Chronic Disease; Fatty Liver; Female; Follow-Up Studies; Hepatitis A; Hepatitis B; Hepatitis B Antigens; Hepatovirus; Humans; Immunodiffusion; Immunoelectrophoresis; Infant; Liver Cirrhosis; Liver Diseases; Liver Regeneration; Male; Middle Aged; Sex Factors

1972
[Experiences with the slide culture used as a preliminary study in bacteriological urine diagnostics].
    Zeitschrift fur die gesamte innere Medizin und ihre Grenzgebiete, 1972, Mar-01, Volume: 27, Issue:5

    Topics: Adolescent; Adult; Agar; Age Factors; Aged; Bacteriological Techniques; Bacteriuria; Child; Chronic Disease; Culture Media; False Positive Reactions; Female; Gels; Humans; Male; Methods; Middle Aged; Pyelonephritis

1972
[Dependence of microbial flora in the bronchial tree of chronic lung disease patients on bronchial secretion and cough symptoms].
    Praxis der Pneumologie, 1971, Volume: 25, Issue:5

    Topics: Agar; Bacteria; Bacteriological Techniques; Blood; Bronchi; Bronchoscopy; Chronic Disease; Cough; Culture Media; Female; Humans; Lung Diseases; Lung Neoplasms; Male; Prospective Studies; Sex Factors; Smoking; Sputum

1971
[Arylamidase and its isoenzymes in diseases of the pancreas and liver].
    Clinica chimica acta; international journal of clinical chemistry, 1971, Volume: 33, Issue:1

    Topics: Acute Disease; Agar; Alanine; Alkaline Phosphatase; Amides; Aminohydrolases; Anilides; Cholestasis; Chronic Disease; Electrophoresis; Hepatitis; Humans; Isoenzymes; Leucine; Liver Cirrhosis; Liver Diseases; Liver Neoplasms; Naphthalenes; Nitro Compounds; Pancreatic Diseases; Pancreatitis

1971
[Agar gel serum protein fractions in different forms of chronic tonsillitis].
    Vrachebnoe delo, 1971, Issue:1

    Topics: Agar; Blood Protein Electrophoresis; Chronic Disease; Gels; Humans; Tonsillitis

1971
The protein fractions in cerebrospinal fluid in the various states of multiple sclerosis.
    European neurology, 1970, Volume: 4, Issue:5

    Topics: Adult; Agar; Age Factors; Blood Protein Electrophoresis; Cell Count; Cerebrospinal Fluid; Cerebrospinal Fluid Proteins; Chemical Precipitation; Chronic Disease; Diagnosis, Differential; Electrophoresis; Female; gamma-Globulins; Gels; Humans; Lymphocytes; Male; Multiple Sclerosis; Sex Factors

1970
Urinary and tissue acid mucopolysaccharides in rat with connective tissue proliferation.
    Clinica chimica acta; international journal of clinical chemistry, 1969, Volume: 26, Issue:3

    Topics: Agar; Animals; Carbon Tetrachloride Poisoning; Chronic Disease; Dialysis; Glycosaminoglycans; Granuloma; Male; Rats; Sulfuric Acids

1969
[Comparative bacteriological studies of non-washed and washed sputum probes of patients suffering from chronic non-tuberculous lung diseases].
    Zeitschrift fur Erkrankungen der Atmungsorgane mit Folia bronchologica, 1969, Volume: 131, Issue:1

    Topics: Agar; Bacteriological Techniques; Chronic Disease; Culture Media; Culture Techniques; Enterobacteriaceae; Humans; Lung Diseases; Methods; Neisseria; Pseudomonas aeruginosa; Sputum; Staphylococcus; Streptococcus

1969
Monoclonal gammopathy (IgG) and chronic ulcerative dermatitis (phagedenic pyoderma).
    Acta medica Scandinavica, 1969, Volume: 186, Issue:4

    Topics: Agar; Blood Protein Disorders; Chronic Disease; Dermatitis; Diagnosis, Differential; Electrophoresis; Female; Humans; Immunoelectrophoresis; Immunoglobulin G; Leg Dermatoses; Middle Aged; Pyoderma

1969
[Alpha-1-antitrypsin deficiency in 100 cases of chronic obstructive bronchopneumopathy].
    Revue de tuberculose et de pneumologie, 1969, Volume: 33, Issue:8

    Topics: Adult; Agar; Bronchial Diseases; Chronic Disease; Electrophoresis; Female; Gels; Humans; Lung Diseases; Male; Metabolism, Inborn Errors; Middle Aged; Pulmonary Emphysema; Respiratory Insufficiency; Trypsin Inhibitors

1969
[Determination of glycoproteins in agar gel in rheumatism and other diseases].
    Vrachebnoe delo, 1967, Volume: 10

    Topics: Adolescent; Adult; Agar; Cholecystitis; Chronic Disease; Diagnosis, Differential; Female; Glycoproteins; Heart Diseases; Humans; Immunodiffusion; Male; Methods; Rheumatic Diseases; Tonsillitis

1967