agar and Chorioamnionitis

Streptococcus agalactiae, also known as Group B Streptococcus (GBS), is a major cause of chorioamnionitis and neonatal sepsis. This study evaluates Raman spectroscopy (RS) to identify spectral characteristics of infection and differentiate GBS from Escherichia coli and Staphylococcus aureus during ex vivo infection of human fetal membrane tissues. Unique spectral features were identified from colonies grown on agar and infected fetal membrane tissues. Multinomial logistic regression analysis accurately identified GBS infected tissues with 100.0% sensitivity and 88.9% specificity. Together, these findings support further investigation into the use of RS as an emerging microbiologic diagnostic tool and intrapartum screening test for GBS carriage.

Topics: Agar; Algorithms; Anti-Bacterial Agents; Chorioamnionitis; Escherichia coli; Escherichia coli Infections; Extraembryonic Membranes; Female; Humans; Logistic Models; Microbiological Techniques; Pregnancy; Regression Analysis; Spectrum Analysis, Raman; Staphylococcal Infections; Staphylococcus aureus; Streptococcal Infections; Streptococcus agalactiae