agar and Autoimmune-Diseases

Topics: Agar; Autoimmune Diseases; Complement System Proteins; Hemolytic Plaque Technique; Humans; Kidney Diseases; Liver Diseases

The soft agar culture assay (C.F.U.-C) has been used in vitro as a measure of haemopoietic capacity of bone-marrow. In a patient with aplastic anaemia pretreatment of the patient's bone-marrow with horse anti-human-thymocyte globulin and complement (A.T.G. + C) prior to culture led to a dramatic increase in ability to form colonies in the soft agar assay; and co-culturing marrow from a normal donor and from the patient resulted in a distinct reduction in the number of expected C.F.U.-C. These findings point ot an immunological or autoimmune mechanism in this patient by selective destruction of the suppressing cells in the patient's marrow with A.T.G. and by suppression of normal myelopoiesis following addition of the patient's marrow to normal marrow.

Topics: Adult; Agar; Anemia, Aplastic; Antilymphocyte Serum; Autoimmune Diseases; Bone Marrow; Cell Count; Clone Cells; Complement System Proteins; Culture Techniques; Female; Hematopoiesis; Hematopoietic Stem Cells; Humans; T-Lymphocytes

Topics: Adenoviridae; Agar; Animals; Antigen-Antibody Complex; Antigens; Autoimmune Diseases; Cattle; Culture Techniques; DNA Viruses; DNA, Viral; Haplorhini; Immune Sera; Immune System Diseases; Immunochemistry; Immunodiffusion; Kidney; Mice; Precipitin Tests; Rabbits; Rats; Satellite Viruses; Ultracentrifugation

Topics: Agar; Agranulocytosis; Autoantibodies; Autoimmune Diseases; Blood Proteins; Child; Electrophoresis; Gels; Humans; Hypersplenism; Purpura, Thrombocytopenic

Topics: Agar; Autoimmune Diseases; Immunodiffusion; Liver Diseases; Rats; Research

Topics: Agar; Antibodies; Autoimmune Diseases; Brain Diseases; Complement Fixation Tests; Hemagglutination; Hemagglutination Tests; Humans; Immunodiffusion