agar and Arthritis--Rheumatoid

To develop a novel immunoadsorbent for rheumatoid arthritis (RA) therapy.. A RA immunoadsorbent was developed by binding heat-aggregated human IgG(HAHIgG) to porous agar gel beads. Its adsorption capacity for rheumatoid factors (RFs), storage stability and blood compatibility were evaluated.. The coupling yield of HAHIgG on the carrier was 6.0 mg/g wet gel. Saturation adsorption capacity of the adsorbent for IgMRF, IgGRF and IgARF were 3400, 2240 and 2400 IU/g, respectively. The adsorbent can be stored at 4 degrees C for three months without significant variance in its activity. Its fine permeability and hemocompatibility were demonstrated by extracorporeal hemoperfusion on rabbits.. HAHIgG/agar gel is a safe and effective immunoadsorbent for RA therapy, its potential clinical use is promising in the future.

Topics: Agar; Animals; Arthritis, Rheumatoid; Dose-Response Relationship, Drug; Drug Storage; Hemoperfusion; Humans; Immunoglobulin G; Immunosorbent Techniques; Immunosorbents; Protein Binding; Rabbits; Rheumatoid Factor

Bone marrow culture in semi-solid agar was used to assess the proliferative activity and the response to sodium aurothiomalate of the myeloid precursor cells from patients during and after recovery from neutropenia associated with the use of this drug. Colony formation was reduced during the neutropenia and returned to normal after recovery. The rheumatoid process itself did not impair colony formation even in patients with Felty's syndrome. Sodium aurothiomalate inhibited colony formation by normal marrow in a dose-dependent manner. Bone marrow colonies from patients who had recovered from neutropenia induced by sodium aurothiomalate were not abnormally sensitive to the inhibitory effect of the drug in vitro. The metabolism of gold is probably altered in a small proportion of patients, which causes high local concentrations within the bone marrow leading directly to marrow depression.

Topics: Adult; Agar; Aged; Agranulocytosis; Arthritis, Rheumatoid; Bone Marrow; Bone Marrow Cells; Cell Division; Clone Cells; Culture Techniques; Dose-Response Relationship, Drug; Felty Syndrome; Gold; Humans; Malates; Middle Aged; Neutropenia; Organometallic Compounds; Sulfides

Topics: Agar; Alleles; Arthritis, Rheumatoid; Diabetes Mellitus; Electrophoresis; Gels; Hepatitis; Humans; Hyperlipidemias; Leprosy; Phenotype; Polymorphism, Genetic; Serologic Tests

Topics: Agar; Animals; Antibodies; Arthritis, Rheumatoid; Cellulose; Chromatography; Complement System Proteins; Erythrocytes; Gels; Hemolysin Proteins; Hemolysis; Hemolytic Plaque Technique; Humans; Immune Sera; Immunodiffusion; Immunoglobulin G; Immunoglobulin M; Mercaptoethanol; Rabbits; Rheumatoid Factor; Sheep; Waldenstrom Macroglobulinemia

Topics: Agar; Animals; Arthritis, Rheumatoid; Beta-Globulins; Complement System Proteins; Hemolysis; Humans; Hydrazines; Immune Sera; Immunoelectrophoresis; Liver Cirrhosis; Multiple Myeloma; Precipitins; Stomach Neoplasms

Topics: Agar; Amyloid; Amyloidosis; Animals; Antigens; Arthritis, Rheumatoid; Fluorescent Antibody Technique; gamma-Globulins; Humans; Immunodiffusion; Methods; Osteomyelitis; Pyelonephritis; Rabbits; Tuberculosis, Osteoarticular; Tuberculosis, Pulmonary

Topics: Agar; Arthritis, Rheumatoid; Humans; Immune Sera; Immunodiffusion; Immunoelectrophoresis; Immunoglobulin G; Immunoglobulin M; Rheumatoid Factor; Ultracentrifugation