agar and Anemia--Sickle-Cell

Both cellulose acetate electrophoresis and citrate agar electrophoresis were performed on 834 blood samples collected on filter paper in Jamaica and shipped for testing to the National Hemoglobinopathy Standardization Laboratory at the U.S. National Center for Disease Control. Additionally, 30 blood samples collected locally were stored on filter paper, in microhematocrit capillary tubes, and as whole blood specimens; at selected times the samples were tested for stability to determine the best sample-collection technique for hemoglobin electrophoresis. Results were most nearly accurate when both cellulose acetate electrophoresis and citrate agar testing were used. The methods are easy to perform, but results are unreliable if the blood samples on filter paper are stored at 4 degrees C for longer than two weeks before they are tested.

Topics: Agar; Anemia, Sickle Cell; Electrophoresis; Electrophoresis, Cellulose Acetate; Female; Fetal Blood; Hemoglobins, Abnormal; Humans; Mass Screening; Methods; Paper; Pregnancy

Topics: Agar; Anemia, Sickle Cell; Blood Protein Electrophoresis; Cellulose; Citrates; Electrophoresis; Evaluation Studies as Topic; Fetal Hemoglobin; Hemoglobin C; Hemoglobin, Sickle; Hemoglobinopathies; Hemoglobins; Hemoglobins, Abnormal; Humans; Infant, Newborn; Methods; Solubility

Topics: Agar; Anemia, Sickle Cell; Blood; Blood Specimen Collection; Cellulose; Electrophoresis; Gene Frequency; Humans; Infant, Newborn; Jamaica; Mass Screening; Methods; Umbilical Cord

Topics: Agar; Anemia, Sickle Cell; Blood Protein Electrophoresis; Evaluation Studies as Topic; Hemoglobins; Hemoglobins, Abnormal; Heterozygote; Homozygote; Humans; Methods; Solubility; Sulfites; Thalassemia; Time Factors

Topics: Acrylates; Adult; Agar; Anemia, Hemolytic; Anemia, Sickle Cell; Blood; Blood Platelets; Blood Protein Electrophoresis; Erythrocytes; Female; Gels; Glucose; Glycolysis; Hexokinase; Humans; Infant, Newborn; Isoenzymes; Leukocytes; Male; Mercaptoethanol; Pregnancy; Spherocytosis, Hereditary; Starch; Thalassemia; Umbilical Cord

Topics: Agar; Anemia, Sickle Cell; Blood; Blood Protein Electrophoresis; Hemoglobins, Abnormal; Humans; Infant, Newborn; Infant, Newborn, Diseases; Umbilical Cord

Agar gel, cellulose acetate, and starch gel electrophoresis are all capable of diagnosing sickle-cell anaemia, sickle-cell haemoglobin C disease, and haemoglobin C disease in cord blood samples. Of these three electrophoretic techniques, agar gel is the easiest to interpret.Paper electrophoresis can reliably and rapidly detect sickle haemoglobin and haemoglobin C in cord blood samples. Being incapable of differentiating foetal and normal adult haemoglobin, the value of paper electrophoresis is limited to an initial screening procedure.

Topics: Agar; Anemia, Sickle Cell; Blood Protein Electrophoresis; Cellulose; Fetal Hemoglobin; Gels; Hemoglobin C; Hemoglobin C Disease; Hemoglobinopathies; Humans; Infant, Newborn; Infant, Newborn, Diseases; Paper; Starch; Umbilical Cord

Topics: Acetates; Adolescent; Adult; Agar; Aged; Amino Acids; Anemia, Sickle Cell; Binding Sites; Black People; Blood Protein Electrophoresis; Cellulose; Child; Child, Preschool; Erythrocytes; Female; Gels; Hemoglobin C; Hemoglobinopathies; Hemoglobins; Hemoglobins, Abnormal; Heterozygote; Homozygote; Humans; Infant; Jamaica; Male; Oxygen; Pedigree; Starch; Thalassemia

Topics: Agar; Anemia, Sickle Cell; Carbonates; Cellulose; Electrophoresis; Fetal Hemoglobin; Glucosephosphate Dehydrogenase; Hemoglobinometry; Hemoglobins, Abnormal; Humans; Magnesium Sulfate; Oxygen Consumption; Sodium

Topics: Agar; Anemia, Sickle Cell; Blood Protein Electrophoresis; Chromatography; Gels; Hemoglobinopathies; Hemoglobins, Abnormal; Humans; Japan; Methemoglobin; Paper; Spectrophotometry