agar and Anemia--Aplastic

The in vitro cloning of haemopoietic precursors is a rapidly growing field. The data reviewed above and the current practical applications of the techniques can be expected to increase quite rapidly in the next decade. Despite the technical problems of tissue culture and the special problems associated with culturing human cells, it is clear that these procedures can be effectively applied at the clinical level. The value of the data obtained will vary directly with the quality of the culture techniques. Any centre undertaking these techniques must be prepared to properly equip the culture laboratory, appoint a full-time staff member for the work and maintain a constant surveillance of the quality of the culture work.

Topics: Agar; Anemia, Aplastic; Cells, Cultured; Colony-Forming Units Assay; Colony-Stimulating Factors; Culture Media; Granulocytes; Hematopoietic Stem Cells; Humans; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Monocytes; Myeloproliferative Disorders; Polycythemia Vera; Preleukemia; Primary Myelofibrosis; Time Factors

Topics: Agar; Anemia, Aplastic; Cell Count; Cell Differentiation; Clone Cells; Colony-Stimulating Factors; Culture Techniques; Hematopoiesis; Hematopoietic Stem Cells; Humans; Leukemia; Neutropenia

Topics: Agar; Anemia, Aplastic; Bone Marrow; Cell Aggregation; Cell Division; Cells, Cultured; Diffusion; Hematopoietic Stem Cells; Humans; Kinetics; Preleukemia

Thirty-seven patients with chronic cytopenia were studied using a CFU-c assay in agar. On the basis of the growth pattern three types of preleukaemic syndrome (PL) and two types of aplastic anaemia were distinguished. Further evaluation of the bone marrow dysfunction was attempted with a combined application of cytochemistry and electron microscopy for the morphologic study of cells proliferating in vitro. Well-defined maturation defects in the growing cells from the bone marrow of patients with PL were demonstrated with cytochemical stainings performed in agar. These results were supported by electron microscopic findings of Auer-body-like inclusions in "statu nascendi" in the vacuoles of preleukaemic cells. On the basis of our results a high risk group of PL for development of overt leukaemia and a group of patients with a grave prognosis in aplastic anaemia were distinguished. The data obtained are relevant for the clinical diagnosis and prognosis of patients with cytopenias.

Topics: Adult; Agar; Aged; Anemia, Aplastic; Bone Marrow Cells; Cells, Cultured; Culture Media; Female; Hematopoietic Stem Cells; Histocytochemistry; Humans; Inclusion Bodies; Male; Microscopy, Electron; Middle Aged; Preleukemia

Topics: Agar; Anemia, Aplastic; Bone Marrow; Cells, Cultured; Colony-Forming Units Assay; Cytological Techniques; Granulocytes; Hematopoiesis; Humans; Leukemia, Lymphoid; Leukemia, Myeloid, Acute

Topics: Agar; Anemia, Aplastic; Cells, Cultured; Child; Colony-Forming Units Assay; Erythropoiesis; Hematopoietic Stem Cells; Humans

Topics: Adolescent; Adult; Agar; Anemia, Aplastic; Bone Marrow; Cell Division; Cells, Cultured; Clone Cells; Culture Media; Female; Granulocytes; Hematopoiesis; Humans; Leukocytes; Male; Middle Aged

The soft agar culture assay (C.F.U.-C) has been used in vitro as a measure of haemopoietic capacity of bone-marrow. In a patient with aplastic anaemia pretreatment of the patient's bone-marrow with horse anti-human-thymocyte globulin and complement (A.T.G. + C) prior to culture led to a dramatic increase in ability to form colonies in the soft agar assay; and co-culturing marrow from a normal donor and from the patient resulted in a distinct reduction in the number of expected C.F.U.-C. These findings point ot an immunological or autoimmune mechanism in this patient by selective destruction of the suppressing cells in the patient's marrow with A.T.G. and by suppression of normal myelopoiesis following addition of the patient's marrow to normal marrow.

Topics: Adult; Agar; Anemia, Aplastic; Antilymphocyte Serum; Autoimmune Diseases; Bone Marrow; Cell Count; Clone Cells; Complement System Proteins; Culture Techniques; Female; Hematopoiesis; Hematopoietic Stem Cells; Humans; T-Lymphocytes

Topics: Adult; Agar; Anemia, Aplastic; Benzene; Blood Protein Electrophoresis; Child; Child, Preschool; Chromatography, DEAE-Cellulose; Elliptocytosis, Hereditary; Erythrocyte Count; Fetal Hemoglobin; Genes; Hematocrit; Hemoglobinometry; Hemoglobins; Hemoglobins, Abnormal; Heterozygote; Humans; Molecular Biology; Starch; Thalassemia

Topics: Agar; Anemia, Aplastic; Anemia, Macrocytic; Child; Fanconi Syndrome; Female; Humans; Immunoelectrophoresis; Male; Prednisone

Topics: Adolescent; Adult; Agar; Anemia, Aplastic; Antibodies; Antibodies, Heterophile; Antigens; Blood Protein Electrophoresis; Blood Sedimentation; Chemical Precipitation; Female; Humans; Immune Sera; Immunoelectrophoresis; Leukemia, Myeloid; Leukocytes; Lupus Erythematosus, Systemic; Male; Serositis