acriflavine and Sarcoma-180

Tumor antigens from Sarcoma-180 ascites tumor cells were extracted by 3 M KCl before and after treatment with acriflavine. Following dialysis of the extracts against distilled water to remove KCl, the soluble material (antigen) and the precipitate formed during dialysis were collected from the dialysate. Protective immunity of the soluble antigen and the precipitate was evaluated by immunizing mice and subsequent challenge with native tumor cells. It was noted that the soluble antigen and the precipitate of the dialysate obtained from acriflavine-treated tumor cells induced significant protective immunity. The precipitate part appeared to be more effective. Interestingly, similar materials from untreated tumor cells did not show any such protective immunizing efficacy. Antigens extracted by 3 M KCl from chemically modified tumor cells assumed significance in respect to their antitumor immunity.

Topics: Acriflavine; Animals; Antigens, Neoplasm; Dialysis; Mice; Potassium Chloride; Sarcoma 180; Vaccines

Phytohemagglutinin (PHA) and concanavalin A (Con A) were used as probes to detect changes in the cell surface of Dalton's lymphoma, sarcoma-180 and Ehrlich's carcinoma after short in vitro exposure to acriflavine. Dye-treated cells showed enhancement of agglutination both by PHA and Con A, and such enhancement was found to be dependent on the time of exposure and concentration of acriflavine. However, PHA-induced percent agglutination seemed to be much higher than that of Con A among the 3 cell types. There were also marked differences among the 3 cell types in order of their sensitivity to lectin-mediated agglutination. The strength of the response was greater in lymphoma to both PHA and Con A than that of sarcoma-180 and carcinoma cells, which appeared to be most resistant. Acriflavine, which is known as an intercalative agent with DNA, induces cell surface changes by promoting lectin-mediated cellular agglutination.

Topics: Acriflavine; Agglutination; Aminoacridines; Animals; Carcinoma, Ehrlich Tumor; Cell Membrane; Cells, Cultured; Concanavalin A; Lymphoma; Male; Mice; Phytohemagglutinins; Sarcoma 180

Topics: Acridine Orange; Acriflavine; Animals; Antigens, Surface; Cell Membrane; Mice; Sarcoma 180