aconitine and Stomach-Ulcer

This study aimed to illustrate the curative effect of Fuzi Lizhong Decoction, which is composed of Aconitum carmichaeli Debeaux, Zingiber officinale Roscoe, Codonopsis pilosula (Franch.), Atractylodes macrocephala Koidz and Glycyrrhiza uralensis Fisch. and contained components such as benzoyl aconitine, benzoyl aconitine, atractyl lactone I, atractylenolide II, ginsenoside, emodin, glycyrrhizin, glycyrrhizin, 8-gingerol and 10-gingerol, on stomach ulcer based on ultra-performance liquid chromatography/mass spectrometry metabolomics analysis. Rats urine samples of three groups were detected by ultra-performance liquid chromatography/mass spectrometry. Then, stomach ulcer biomarkers were filtered out by EZinfo 2.0 software. After that, analysis of variance was used for picking up significantly retroved biomarkers in treated group. Compounds were identified based on MS/MS spectrum, accurate mass weight and retention time. Twelve metabolites including 2-Methylhippuic acid, Kynurenic acid, 2-Indolecarboxylic acid, Pyrocatechol, Caproic acid, 2,3-Dihydrobenzofuran, 4-Ethylphenol, Lanthionine ketimine, Traumatic acid, Indole-3-carboxilic acid-O-sulphate, Vanillic acid 4-sulfate, 6-Mecaptopurine ribonudeoside 5'-diphosphate were identified as stomach ulcer biomarkers. Among them, ten ones except for 2-Methylhippuic acid and 2-Indolecarboxylic acid retrieved. Pathways involved tryptophan metabolism, benzoate degradation, bisphenol degradation and α-linolenic acid metabolism. In conclusion, Fuzi Lizhong Decoction may cure stomach ulcer by improving tryptophan metabolism, benzoate degradation, bisphenol degradation and α-linolenic acid metabolism.

Topics: Aconitine; alpha-Linolenic Acid; Animals; Benzoates; Biomarkers; Chromatography, High Pressure Liquid; Diterpenes; Drugs, Chinese Herbal; Glycyrrhizic Acid; Medicine, Chinese Traditional; Rats; Stomach Ulcer; Tandem Mass Spectrometry; Tryptophan

Various new butyridenyl-2-hydroxybenzylidenyl-1,3-thiazolidinones were synthesized and characterized by elemental analyses, IR and PMR spectral data. The compounds were evaluated for their ability to afford protection against inflammation by carrageenin-induced oedema and cotton pellet implantation in albino rats of either sex. The active derivatives of the present series were also tested for their analgesic activity against aconitine-induced writhing in albino mice and ulcerogenic activity in albino rats. The toxicity of the compounds was reflected by determination of their approximate LD50 in albino mice. An attempt has also been made to correlate their structure-activity relationship.

Topics: Aconitine; Animals; Anti-Inflammatory Agents, Non-Steroidal; Carrageenan; Chemical Phenomena; Chemistry; Edema; Female; Gossypium; Lethal Dose 50; Male; Mice; Rats; Stomach Ulcer; Thiazoles