acid-phosphatase and Wilms-Tumor

Nine surgically resected Wilms' tumors (WIT) and nude mouse heterotransplants from one WIT were studied by histochemistry and immunohistochemistry. Histochemistry showed acid phosphatase in all cells, while alkaline phosphatase and gamma-glutamyl transpeptidase were present in only some tubules. Using immunohistochemistry, antibodies to the intermediate filaments cytokeratin and vimentin distinguished tubular epithelium and mesenchyme, respectively. WIT tubules were also identified using antibody against a structural component (epithelial membrane antigen) and a secretory product (uromucoid) associated with distal convoluted tubules of normal kidney. Basement membrane surrounding the tubules of WIT was demonstrated using antibody to type IV collagen plus laminin. Different blastema subpopulations were negative or stained positively with antibodies to cytokeratin and vimentin. Production of basement membrane by blastema was also shown. Fetal antigen expression in WIT was examined using the monoclonal PI 153/3 and J5 antibodies. The blastema and tubules of WIT were strongly stained by PI 153/3, which did not label normal adult kidney, and weakly stained by J5, which strongly labeled glomeruli and proximal convoluted tubules of normal kidney. These studies show that WIT blastema is heterogeneous in intermediate filament subtypes, while WIT tubules more closely resemble distal than proximal convoluted tubules of adult kidneys but also retain expression of fetal antigens.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Histocytochemistry; Humans; Immunoenzyme Techniques; Keratins; Kidney Neoplasms; Membrane Proteins; Mice; Mice, Nude; Mucin-1; Neoplasm Transplantation; Rabbits; Transplantation, Heterologous; Vimentin; Wilms Tumor

Interest in the role of mononuclear phagocytes in glomerulonephritis (GN) and in defining markers of renal neoplasms led the authors to study alpha-naphthyl acetate/butyrate esterase (ANAE/ANBE), alkaline phosphatase (AlkP), acid phosphatase (AcP), 5'-nucleotidase (5'N), and ATPase (ATP) activity in paraformaldehyde-fixed, plastic-embedded renal tissue from patients with a variety of pathologic conditions. These conditions included GN, renal tumors, and transplant rejection. Enzymatic staining for ANAE, ANBE, AcP, AlkP, and ATP was generally confined to tubules and collecting ducts in normal kidney. Nine of 10 cases of renal carcinoma had weakly to strongly positive reactions with AlkP, AcP, and ANAE; 9 of 10 cases of Wilms' tumor showed weakly positive reactions with AcP and ANAE, particularly in tubular structures. Severely damaged kidney allografts showed surprising retention of normal histochemical features. In all cases 5'N stained both glomerular capillaries and interstitial vasculature; ATPase and AlkP stained interstitial vessels only. Plastic embedding provides superb preservation of both microscopic anatomy and enzymatic activity, which may allow utilization of enzyme histochemistry for diagnostic and research purposes.

Topics: 5'-Nucleotidase; Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Carboxylic Ester Hydrolases; Carcinoma, Renal Cell; Glomerulonephritis; Histocytochemistry; Humans; Kidney; Kidney Diseases; Kidney Glomerulus; Kidney Neoplasms; Microtomy; Naphthols; Nucleotidases; Plastics; Staining and Labeling; Wilms Tumor

Topics: Acid Phosphatase; Clinical Enzyme Tests; Eye Diseases; Genes, Dominant; Gonadal Dysgenesis; Humans; Iris; Isoenzymes; Kidney Neoplasms; L-Lactate Dehydrogenase; Pedigree; Syndrome; Wilms Tumor

An immunoperoxidase technique to detect prostatic-specific acid phosphatase (PSAP) was used on specimens from 98 cases of prostatic carcinoma that were graded by both the Gleason and the Mostofi systems, to see if tumor grade correlated with amount of PSAP seen in tissue. Most tumors showed strong, diffuse cytoplasmic staining; no significant difference was seen among the various grades. Other than focal, weak staining of renal tubular epithelium, the antibody to PSAP gave uniformly negative results with a variety of normal and neoplastic tissues. In light of the great sensitivity and specificity of this technique, it potential applications include diagnosis of poorly differentiated prostatic malignant neoplasms, whether primary or metastatic.

Topics: Acid Phosphatase; Adenocarcinoma; Carcinoma, Intraductal, Noninfiltrating; Carcinoma, Squamous Cell; Humans; Immunoenzyme Techniques; Male; Melanoma; Neoplasm Staging; Prostatic Neoplasms; Wilms Tumor

Topics: Acid Phosphatase; Female; Genetic Linkage; Humans; Infant; Iris; L-Lactate Dehydrogenase; Male; Wilms Tumor

The hydrolases of acid and alkaline phosphatase and non-specific esterases were investigated by histochemical and electrophoretic techniques in normal and neoplastic renal tissues and their cultured cells. A distinct tumour specific pattern of non-specific esterases was demonstrated in Wilms' tumour. The reasons for the quantitative differences found in the distribution of alkaline phosphatase in Wilms' tumour and their cultured cells are discussed.

Topics: Acid Phosphatase; Adenocarcinoma; Alkaline Phosphatase; Culture Techniques; Esterases; Humans; Isoenzymes; Kidney; Kidney Neoplasms; Wilms Tumor

Topics: Acid Phosphatase; Adolescent; Adult; Age Factors; Child; Child, Preschool; Female; Humans; Hydronephrosis; Infant; Kidney; Kidney Calculi; Kidney Diseases; Kidney Neoplasms; Male; Nephritis; Sex Factors; Wilms Tumor

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Deoxyribonucleases; Histocytochemistry; Male; Neoplasms, Experimental; Nitrosamines; Rats; Ribonucleases; Wilms Tumor