acid-phosphatase and Varicocele

Varicocele has been associated with decreased semen quality, not much is known about the effect of varicocele on the accessory sex glands function. The purpose of this study was to evaluate the relationship among varicocele, seminal parameters and biochemical markers of accessory sex glands: neutral alpha glucosidase (NAG, epididymis), fructose (seminal vesicles), prostatic acid phosphatase (PAP) and zinc (prostate).. A clinical study was performed in 190 men with varicocele and 100 men normozoospermic as control group. Semen analysis, hypoosmotic swelling test (HOST), polymorphonuclear (PMN), fructose, zinc, PAP and NAG were determinate. Differences were evaluated by, t test, ANOVA and a Pearson's coefficient correlation.. Varicocele group showed a decrease in sperm motility, normal morphology, HOST and vitality. No differences were observed in fructose, PAP and zinc levels between control and varicocele group. The NAG was significantly decreased in varicocele group. A positive correlation was observed between both fructose and PAP with semen volume, sperm concentration, PMN, and zinc levels. Additionally, a decrease of NAG was correlated with a decrease of normal sperm morphology, motility, vitality and HOST.. Varicocele does not alter fructose secretion by seminal vesicles and PAP and zinc by prostate. Varicocele is associated with a decrease of NAG activity in seminal fluid, suggesting epididymal dysfunction possibly associated with a detrimental in sperm quality.

Topics: Acid Phosphatase; Adult; alpha-Glucosidases; Biomarkers; Epididymis; Fructose; Genitalia, Male; Humans; Male; Prostate; Protein Tyrosine Phosphatases; Semen Analysis; Seminal Vesicles; Varicocele; Zinc

To compare the sperm protein profile between men with and without varicocele.. The present study was designed as a case-control study. The research patients were recruited from the Infertility Unit of the Royan Institute in 2009. We included 20 sperm samples from normozoospermic men without varicocele (control group) and 20 sperm samples from oligozoospermic patients with varicocele, grade 3 (varicocele group) in the present study. The sperm protein profile in the 2 groups was characterized using 2-dimensional gel electrophoresis. Differences in protein expression were established using gel analysis software, and protein identification was performed using mass spectroscopy analysis.. In the varicocele group, we noted 15 consistent differences in protein expression (1, spots missing; 12, less abundant; and 2, more abundant) compared with the control group (P < .01). The findings revealed that heat shock proteins, mitochondrial proteins, and cytoskeleton proteins are the proteins mainly affected by varicocele disease.. To our knowledge, the present study is a novel study, with few studies describing the correlation between sperm protein in men with and without varicocele obtained using a 2-dimensional proteomic approach. It could be an important prerequisite to the development of diagnostic tests to predict varicocelectomy outcomes in patients with varicocele and abnormal findings on a spermogram in the clinical environment.

Topics: Acid Phosphatase; Adult; Carrier Proteins; Case-Control Studies; Clusterin; Cytoskeletal Proteins; Electrophoresis, Gel, Two-Dimensional; Endoplasmic Reticulum Chaperone BiP; Glycoproteins; Heat-Shock Proteins; Humans; Intracellular Signaling Peptides and Proteins; Kallikreins; Male; Membrane Transport Proteins; Mitochondrial Proteins; Mitochondrial Proton-Translocating ATPases; Oligospermia; Oncogene Proteins; Prostate-Specific Antigen; Protein Deglycase DJ-1; Protein Precursors; Protein Tyrosine Phosphatases; Proteomics; Proton-Translocating ATPases; Seminal Vesicle Secretory Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spermatozoa; Superoxide Dismutase; Superoxide Dismutase-1; Varicocele

The present study was undertaken to determine the effects of surgically induced varicocele on testicular androgen production, seminiferous tubular diameter, and acid and alkaline phosphatase activities in testis and accessory sex organs in adult Noble rats. Testosterone concentrations in plasma and in both left and right testis were estimated in both sham-operated and varicocele-induced rats. The activities of acid and alkaline phosphatase were measured in both left and right testis, seminal vesicle, and ventral prostate gland in varicocele-induced and sham-operated rats. The levels of testosterone in plasma in rats with varicocele were lower than those in the sham-operated rats. The concentration of testosterone in the left testis was lower than that in the right testis in varicocele-induced rats while no such differences were observed in sham-operated rats. The seminiferous tubular diameter in the left testis is reduced in varicocele-induced rats from that in the right testis. In the sham animals, testicular acid phosphatase activity was higher in the left testis than the right, while in varicocele-induced animals, no differences in acid phosphatase activity between left and right testis were observed. In varicocele-created rats, the activity of alkaline phosphatase was higher in the left testis than in the right while no differences in alkaline phosphatase activity between left and right testis in sham-operated rats were observed.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Genitalia, Male; Male; Organ Size; Rats; Testis; Testosterone; Varicocele

Sperm examination, quantitative sperm culture, citric acid, acid phosphatase, and fructose were assayed in three groups of men: fertile controls without significant bacteriospermia (group I), infertile men with significant bacteriospermia; idiopathic infertile men (group II), and infertile men with varicocele (group III). Level of significance of bacteriospermia was greater than or equal to 10(4) germs/ml of ejaculate. In group II, motility and typical morphology percentages were lower, independently of the degree and the nature of bacteriospermia. Incidence of pathogenic bacteria was higher than in group III and linked to the degree of bacteriospermia. Fructose was unaltered in the two groups of infected men. No modification of prostatic markers was observed in any groups, except in group II, where they decreased when bacteriospermia was lower than 10(5) germs/ml and when biological pattern of semen evoked chronic prostatitis. Thus, the presence of germs in ejaculate alters the motility and the typical morphology percentages but does not result in any obvious modifications of biochemical markers of prostate and seminal vesicles. For idiopathic infertile men, it is suggested that the quantitative criterion of pathogenic bacteriospermia is a germ count greater than or equal to 10(5)/ml.

Topics: Acid Phosphatase; Adult; Citrates; Citric Acid; Fructose; Genital Diseases, Male; Humans; Infertility, Male; Male; Middle Aged; Prostate; Semen; Seminal Vesicles; Sperm Count; Spermatozoa; Varicocele

Levels of zinc and magnesium were estimated in the seminal plasma of 83 men having various infertility problems. There was a wide overlapping of Zn and Mg mean values among the groups of normal and infertile men, a significant reduction in Mg mean value (but not Zn) only in the group with infection of the accessory genital glands, a very high positive correlation between Zn and Mg mean values in all groups, and a high positive correlation between either Zn or Mg and acid phosphatase (but neither fructose nor sperm count and forward progression) in men with infection, but not in the other groups. Measurement of Mg in seminal plasma can, in combination with other parameters of prostatic function, give useful information regarding male infertility problems, particularly in the case of infection of the accessory genital glands.

Topics: Acid Phosphatase; Adult; Humans; Infections; Infertility, Male; Magnesium; Male; Middle Aged; Semen; Sperm Count; Sperm Motility; Varicocele; Zinc

The semen from 20 men with varicocele was studied before and after surgical correction of the varicocele. The effect on sperm quality was only marginal and could not explain the fairly high conception rate (20%) after operation. The recommendation that varicocele in men with long-term infertility should be eliminated by ligation of the internal spermatic veins is still justified despite the absence of positive effects on sperm quality.

Topics: Acid Phosphatase; Adult; Follow-Up Studies; Fructose; Humans; Hydrogen-Ion Concentration; Male; Semen; Sperm Motility; Varicocele; Zinc

Plasma testosterone concentration was decreased in 10 patients combining varicocele with sexual inadequacy (mean 346.2 ng/100 ml) against normal concentration observed in 23 men with varicocele without sexual disturbances (mean 567.8 ng/100 ml) and in 31 patients with pure psychogenic impotence (mean 581.6 ng/100 ml). There was a significant inverse linear correlation between age and plasma testosterone concentration in the varicocele patients (r= minus 0.56, P smaller than 0.01) in contrast to the absence of such correlation in normal men or in patients with psychogenic impotence of the same age range. The secretion products of the secondary sex glands were more often in the lower range in the ejaculates of men combining varicocele with sexual disturbance (P smaller than 0.02), proving the decreased testosterone level to induce a deficient function of these glands. Plasma testosterone levels normalized after surgical correction in varicocele patients with a low preoperative concentration. Since adequate surgical or hormonal treatment resulted in complete recovery of sexual potency in the majority of patients with varicocele and sexual inadequacy, it is suggested that the decreased testosterone production might have contributed to the impairment of sexual function.

Topics: Acid Phosphatase; Adolescent; Adult; Age Factors; Aged; Erectile Dysfunction; Female; Fructose; Humans; Hydrogen-Ion Concentration; Male; Middle Aged; Prostate; Semen; Sexual Dysfunction, Physiological; Sperm Motility; Spermatozoa; Testosterone; Varicocele

Topics: Acid Phosphatase; Adult; Humans; Infertility, Male; Male; Middle Aged; Semen; Spermatozoa; Testis; Testosterone; Varicocele

Topics: Acid Phosphatase; Androgens; Biopsy; Ejaculation; Histocytochemistry; Humans; Male; Spermatozoa; Steroids; Testis; Varicocele