acid-phosphatase and Uveitis

Acute uveal inflammation, characterised by conjunctival hyperaemia, limbal vascular injection, iritis and aqueous flare, was induced in the rabbit eye by a single intravitreal injection of 10 micrograms of Shigella endotoxin. Twenty-four, 48 and 72 h after endotoxin administration, lysosomal-rich fractions and cytosolic supernatants were prepared from iris-ciliary bodies and the enzymatic activities of some lysosomal hydrolases in these fractions were assayed. Acid phosphatase, aryl sulphatase and N-acetyl-B-D-glucosaminidase exhibited increased activities in the lysosomal preparations from inflamed iris-ciliary bodies, relative to the activities of the same enzymes in lysosomal fractions from normal iris-ciliary bodies. No significant differences in activities were obtained for these enzymes in cytosolic supernatants from normal and inflamed iris-ciliary bodies. The results suggest that during acute ocular inflammation the lysosomal hydrolases are retained in their organelles at an elevated state of activity.

Topics: Acetylglucosaminidase; Acid Phosphatase; Animals; Arylsulfatases; Ciliary Body; Cytosol; Endotoxins; Glucuronidase; Iris; Lysosomes; Organelles; Rabbits; Shigella; Uveitis

Topics: Acid Phosphatase; Aminopeptidases; Animals; Arthus Reaction; Cathepsin D; Cathepsin H; Cathepsins; Cysteine Endopeptidases; Lysosomes; Rabbits; Uveitis

Topics: Acid Phosphatase; Animals; Autoimmune Diseases; Guinea Pigs; Retinitis; Uveitis

Acid phosphatase and beta-glucuronidase were used as marker enzymes of lysosome, and their role in adjuvant-induced uveitis was studied. These enzyme activities in the iris-ciliary processes were increased in the inflamed tissues. Changes in these enzyme activities in the tissues paralleled the development of uveitis. While protein concentration in the aqueous humor as a parameter of vascular permeability was significantly correlated with these enzyme activities, there was no correlation in the leucocyte counts in the aqueous humor. Topically applied dexamethasone reduced the increase in the aqueous protein, the leucocyte migration and the swelling of the iris-ciliary processes, while topically applied indomethacin reduced only the leucocyte migration among these inflammatory parameters. Acid phosphatase activities in the inflamed tissues were reduced also by dexamethasone, but not by indomethacin.

Topics: Acid Phosphatase; Adjuvants, Immunologic; Animals; Anti-Inflammatory Agents; Dexamethasone; Disease Models, Animal; Glucuronidase; Indomethacin; Iris; Lysosomes; Male; Rabbits; Uveitis

Canine adenovirus-type 1 (CAV-1)-antibody complexes caused severe anterior uveitis with corneal edema ("blue eye") when injected into the anterior chamber of normal dogs. The response of the anterior uvea to such immune complexes (IC) was similar to the spontaneously occurring disease. In the presence of complement (C'), IC caused release of neutrophile chemotactic factors. Following phagocytosis of IC-C' leukocytes released lysosomal enzymes, as indicated by the presence of acid phosphatase in the surrounding medium. Membrane bound viral aggregates, presumably IC, were common in neutrophiles and in macrophages that had infiltrated the anterior chamber of opaque eyes that occurred after intravenous (IV) inoculation with attenuated CAV-1. These data were incorporated into a postulated scheme for the pathogenesis of CAV-1 uveitis with corneal edema.

Topics: Acid Phosphatase; Animals; Antigen-Antibody Complex; Antigens, Viral; Chemotaxis; Complement Fixation Tests; Complement System Proteins; Cornea; Dogs; Eye Diseases; Fluorescent Antibody Technique; Hepatitis, Infectious Canine; Keratitis; Leukocytes; Lysosomes; Macrophages; Neutrophils; Uveitis