acid-phosphatase and Uterine-Cervical-Neoplasms

Topics: Acid Phosphatase; Adenocarcinoma; Alkaline Phosphatase; Aminopeptidases; Bone Neoplasms; Breast Neoplasms; Cervix Uteri; Clinical Enzyme Tests; Diagnosis, Differential; Esterases; Female; Glucosephosphate Dehydrogenase; Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; Hyperplasia; Microscopy, Fluorescence; Neoplasms; Nucleic Acids; Sex Chromatin; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Alanine Transaminase; Alkaline Phosphatase; Aspartate Aminotransferases; Blood; Cobalt Radioisotopes; Extracorporeal Circulation; Female; Humans; Isoenzymes; Radiation Effects; Radioisotope Teletherapy; Radiotherapy Dosage; Radium; Time Factors; Uterine Cervical Neoplasms

Adenoid basal cell carcinoma (ABC) is considered a rare cervical neoplasm which when present in 'pure' form, uniquely amongst apparently malignant cervical tumours, has never been reported to metastasise or lead to fatal patient outcome. We recently encountered a case of ABC that was morphologically reminiscent of prostatic differentiation, more specifically basal cell hyperplasia of the prostate. Immunohistochemistry was strongly positive for the prostate related marker NKX3.1 in the glandular cells, but there was no expression of prostate specific antigen (PSA) or prostatic acid phosphatase (PAP). However, subsequent review of five additional cervical ABCs demonstrated focal PAP expression in two of four tested cases, and all were NKX3.1 positive. NKX3.1 expression was also demonstrated in the glandular epithelium of 10 additional gynaecological lesions considered to show prostatic differentiation including five cases of cervical ectopic prostatic tissue, three ovarian teratomas with prostatic differentiation, and two vaginal tubulosquamous polyps. We suggest that some lesions traditionally classified as ABC may in fact represent a variant of prostatic differentiation within the cervix, possibly analogous to basal cell hyperplasia of the prostate.

Topics: Acid Phosphatase; Aged; Biomarkers, Tumor; Carcinoma, Basal Cell; Cell Differentiation; Cervix Uteri; Female; Homeodomain Proteins; Humans; Hyperplasia; Immunohistochemistry; Male; Middle Aged; Prostate; Prostate-Specific Antigen; Prostatic Neoplasms; Transcription Factors; Uterine Cervical Neoplasms

To prospectively investigate the changes in bone mineral density (BMD) after pelvic radiation therapy in patients with uterine cervical cancer.. Of 52 cervical cancer patients who received pelvic RT in our university hospital between 2009 and 2011, 46 patients without recurrence and who were followed up for more than 12 months were included in the study. The BMD of the irradiated region and nonirradiated regions, serum estradiol, tartrate-resistant acid phosphatase-5b, and N-terminal cross-linking telopeptide of collagen 1 were measured before, at 3 months after, and at 12 months after RT. The patient cohort was divided into 2 groups according to estradiol level before RT, and the groups were defined as postmenopausal (<40 pg/mL) and premenopausal (≥40 pg/mL).. The mean BMDs within the irradiation field (lumbar vertebra 5) in the postmenopausal and the premenopausal groups were 0.825 and 0.910 g/cm(2) before RT and 0.746 and 0.841 g/cm(2) 12 months after RT, respectively. Significant decreases were observed in both groups (P<.05 and P<.01, respectively). In addition, in the premenopausal group the mean BMDs of the nonirradiated regions at thoracic vertebrae 9-12 and lumbar vertebrae 2-4 were 0.753 and 0.958 g/cm(2) before RT and were significantly decreased to 0.706 and 0.921 g/cm(2) 12 months after RT (P<.01 and P<.05, respectively). Estradiol significantly decreased 3 months after RT, whereas tartrate-resistant acid phosphatase-5b and N-terminal cross-linking telopeptide of collagen 1 continued to increase over time in the premenopausal group.. A decrease in BMD in the irradiated region after RT was observed within 1 year, regardless of menopausal status. Furthermore, in premenopausal patients, pelvic RT caused a decrease in systemic BMD.

Topics: Acid Phosphatase; Adenocarcinoma; Adult; Aged; Aged, 80 and over; Antineoplastic Agents; Biomarkers; Bone and Bones; Bone Density; Brachytherapy; Carcinoma, Squamous Cell; Collagen Type I; Combined Modality Therapy; Estrogens; Female; Humans; Isoenzymes; Lumbar Vertebrae; Middle Aged; Pelvis; Peptides; Postmenopause; Premenopause; Prospective Studies; Radiotherapy Dosage; Tartrate-Resistant Acid Phosphatase; Thoracic Vertebrae; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Adult; Cervix Uteri; Choristoma; Female; Humans; Immunoenzyme Techniques; Male; Prostate; Prostate-Specific Antigen; Protein Tyrosine Phosphatases; Uterine Cervical Dysplasia; Uterine Cervical Neoplasms; Uterine Diseases

Topics: Acid Phosphatase; Adenocarcinoma; Carcinoma in Situ; Carcinoma, Adenoid Cystic; Carcinoma, Basal Cell; Cervix Uteri; Choristoma; Diagnosis, Differential; Female; Humans; Male; Middle Aged; Prostate; Prostate-Specific Antigen; Protein Tyrosine Phosphatases; Uterine Cervical Diseases; Uterine Cervical Neoplasms

Adenocarcinomas may arise primarily from the urinary bladder, but secondary involvement from adenocarcinomas arising in adjacent organs is more common. In the present study we tried to differentiate primary urinary bladder adenocarcinomas from adenocarcinomas arising from the surrounding organs, based on their antigen profiles in routinely processed, paraffin-embedded tissue specimens. We analysed the staining results using stepwise linear discriminant analysis.. We investigated the usefulness of a panel of antibodies against cytokeratin 7, E48, cytokeratin 20, PSA, PSAP, CEA, vimentin, OC125 and HER-2/neu, to discriminate primary bladder adenocarcinoma from adenocarcinomas arising from the prostate, urachus, colon, cervix, ovary and endometrium. In the differential diagnosis with urinary bladder adenocarcinoma, an overall correct classification was reached for 77% and 81% of urachal and colonic carcinomas, respectively, using CEA, for 93% of prostatic adenocarcinomas using PSA, for 82% and 70% of cervical and ovarian adenocarcinomas, respectively, using OC125, and for 91% of endometrial adenocarcinomas using vimentin. Adding other antibodies did not improve the classification results for any of these differential diagnoses.. For the surgical pathologist, a panel of antibodies consisting of CEA, PSA, OC125 and vimentin is helpful to differentiate primary urinary bladder adenocarcinomas from adenocarcinomas originating from prostate and endometrium, less helpful in differentiation with urachal carcinoma, and not helpful in differentiation with colonic, cervical and ovarian carcinoma.

Topics: Abdominal Neoplasms; Acid Phosphatase; Adenocarcinoma; Antibodies, Monoclonal; Antibody Specificity; CA-125 Antigen; Carcinoembryonic Antigen; Carcinoma, Papillary; Cell Adhesion Molecules; Diagnosis, Differential; Endometrial Neoplasms; Female; Glycoproteins; GPI-Linked Proteins; Humans; Immunohistochemistry; Intermediate Filament Proteins; Keratin-20; Keratin-7; Keratins; Male; Neoplasms, Unknown Primary; Ovarian Neoplasms; Prostate; Prostate-Specific Antigen; Receptor, ErbB-2; Urachus; Urinary Bladder Neoplasms; Uterine Cervical Neoplasms; Vimentin

Topics: Acid Phosphatase; False Negative Reactions; Female; Humans; MEDLINE; Neoplasm Invasiveness; Uterine Cervical Dysplasia; Uterine Cervical Neoplasms; Vaginal Smears

The important components of mucopolysaccharides and collagen have been analyzed in tissues of control and carcinoma of uterine cervix. Among these components hyaluronic acid and chondroitin sulphate levels were found to be increased, whereas decreased level of collagen was observed in uterine cervical carcinoma. Serum cathepsin B, D and acid and alkaline phosphatases have also been analyzed in controls and carcinoma patients before and after treatments. The activities of these enzymes have been found to increase prominently in advanced stages. Among these enzymes cathepsin B and alkaline phosphatase have exhibited remarkable increase in activity in uterine cervical carcinoma. Different modes of treatment exerted reversion of the elevated activities of these enzymes. However, combined therapy type II (radiation combined with cisplatin and cyclophosphomide) seems to be more effective in reverting the activities of these enzymes to normal levels.

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Cathepsins; Chondroitin Sulfates; Collagen; Dermatan Sulfate; Female; Glycosaminoglycans; Heparitin Sulfate; Humans; Middle Aged; Pepsin A; Peptide Hydrolases; Uterine Cervical Neoplasms

In patients with precancerous states and cancer of the uterine cervix prior to and after radiotherapy exhibit the decreased activity of neutrophil beta-glucuronidase. Moreover, patients treated by radiotherapy before the age 6 to 9 years demonstrate deficiency of N-acetyl-beta-glucuronidase in the above cells. The main finding in lymphocytes of the patients studied was in the appearance by diffusion of the above enzymes and of acid phosphatase in the cytoplasm, reflecting their release from lysosomes and immunological mobilization of these cells. The authors discuss the possible role of neutrophil enzymatic deficiency in lowering the antitumor cytotoxic effect of these cells.

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Alkaline Phosphatase; Brachytherapy; Female; Glucuronidase; Humans; Middle Aged; Neutrophils; Precancerous Conditions; Radiotherapy Dosage; Uterine Cervical Neoplasms

This research report studies several biochemical and histochemical aspects of cervical carcinoma and explores their use in follow-up of patients undergoing radiotherapy. Material came from 19 patients with invasive cervical carcinoma admitted to Kenyatta National Hospital. A control group consisted of 20 women matched for age who attended clinics at the hospital but were not suffering from any malignant disease; control tissue for histological examination was obtained from 3 women who had undergone hysterectomy for uterine fibroids. Biochemical assays for alkaline and acid phosphatases in patients with cervical carcinoma show an increase in alkaline phosphatase in carcinomatous tissue (35.7 umoles/hr/mg) as opposed to normal tissue (7.2). Acid phosphatase values were only moderately raised. Assays of the same enzymes in blood showed a less marked difference between patients and controls (ranges of 7.5-20.8 and 3-14, respectively). When examined histochemically, increased alkaline phosphatase activity was observed in connective tissue, epithelium of the glands and blood capillaries of tumor tissue. 1 section containing normal tissue bordering carcinomatous tissue demonstrated normal alkaline phosphatase activity in the normal tissue and increased activity in the tumor tissue. In summary, there is increased enzyme activity around the tumor areas, but values for serum levels show an overlap of normal and abnormal cases and are therefore not predictive. Results demonstrate a clear difference in activities of these enzymes in carcinomatous tissue and normal tissue, which may be of value in follow-up care.

Topics: Acid Phosphatase; Adenosine Triphosphate; Alkaline Phosphatase; Carcinoma; Female; Histocytochemistry; Humans; Kenya; Uterine Cervical Neoplasms

A case of a pure heterologous sarcoma of the uterine corpus composed exclusively of rhabdomyosarcomatous elements has been studied by multiple morphologic and biochemical techniques. The neoplasm filled the endometrial cavity and protruded out the cervical os, but the myometrium was only superficially invaded. The tumor did not extend outside the corpus. The pathologic features are discussed in detail. Evidence of striated muscle differentiation could be identified on light microscopic and ultrastructural examination. Immunoperoxidase staining of tumor cells with antibodies to myoglobin were positive. Histochemical preparations for lactate dehydrogenase, succinic dehydrogenase, and acid phosphatase were also positive in neoplastic cells. Other stains gave equivocal or negative results. These findings are discussed in comparison with previous reports.

Topics: Acid Phosphatase; Aged; Female; Histocytochemistry; Humans; Hysterectomy; Immunoenzyme Techniques; L-Lactate Dehydrogenase; Microscopy, Electron; Neoplasm Invasiveness; Rhabdomyosarcoma; Succinate Dehydrogenase; Uterine Cervical Neoplasms; Uterine Neoplasms

In this study histologic and histochemical examinations were performed on the bioptic material of 30 cases of head and neck tumors and 34 cases of uterocervical carcinomas after treatment with human leukocyte interferon (HLI). Particular attention was paid to the interaction between stromal and neoplastic cells as a possible host response to the treatment with interferon. In addition to tumor biopsies, regional lymph nodes were also examined microscopically. Stromal cells (macrophages, T and B lymphocytes, and granulocytes) were detected by enzyme-histochemical methods. Histologic and histochemical criteria for classification of tumor biopsies into five groups included the frequency, the cellular composition of peritumoral stroma, the grade of macrophage infiltration into the tumor (expressed quantitatively), and cytologic changes, followed by the regression of tumor. The overall appraisal of patients with cervical carcinoma based on histopathologic and chemical criteria showed the positive effect of HLI therapy in 70% of the cases; observations of head and neck carcinoma treated with HLI showed positive effects in 82% of the cases.

Topics: Acid Phosphatase; Female; Head and Neck Neoplasms; Histocytochemistry; Humans; Interferons; Peroxidase; Uterine Cervical Neoplasms

Enzymatic equipment in peripheral blood neutrophils has been determined in 10 women with cervical carcinoma, 5 with carcinoma of the uterus body and 30 women with myomas of the uterus. Using cytochemical techniques the authors observed the intracellular deficiency of N-acetyl-beta-glucosaminidase accompanied by diminished absolute count of the enzyme-positive cells as well as the beta-glucuronidase- and the myeloperoxidase-positive neutrophils. Lowered activity of the myeloperoxidase was another pattern of cells in question. The acid phosphatase activity of neutrophils in the patients was significantly elevated. It has been suggested that observed intracellular deficiencies of selected enzymes within the neutrophils are of importance with regard to lowered antitumor activity of that cells operating mainly through the myeloperoxidase-H2O2-halide system.

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Female; Glucuronidase; Glycogen; Humans; Leiomyoma; Middle Aged; Neutrophils; Peroxidase; Uterine Cervical Neoplasms; Uterine Neoplasms

A comparative study was made of the total lysosomal enzyme activity found in homogenates of normal ectocervical squamous epithelium and squamous carcinoma of this epithelium. The activities of acid phosphatase, beta-glucuronidase, cathepsin D and acid ribonuclease were higher in carcinoma tissue than in normal tissue. The most important observation made was with regard to the distribution of enzyme activity in homogenates. In carcinoma homogenates most of the enzyme activity was detected in the lysosomal fractions, whereas in controls the activity was predominantly found in the cytosol fractions. No histochemical and electron microscopical techniques were used in this study. Because it was possible to sediment the enzyme activity and to demonstrate latency, these can be referred to as lysosomal enzymes with certainty.

Topics: Acid Phosphatase; Carcinoma, Squamous Cell; Cathepsins; Cervix Uteri; Cytosol; Female; Glucuronidase; Lysosomes; Ribonucleases; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Adult; Aged; Female; Humans; Lysosomes; Middle Aged; Uterine Cervical Neoplasms; Vagina; Vaginal Smears

In a review of histologic sections of regional lymph nodes removed during surgery in the course of invasive cancer of the uterine cervix from 84 patients there have been distinguished four basic stages of immunologic response. Active immune response (I and II stage) was detected in all patients with non-metastatic cancer and minimal stromal invasion and 41% of patients with advanced invasion. The regional lymph nodes, in these cases, exhibited increased number of small primary or secondary lymph follicles, proliferative sinus histiocytosis and expanded thymus--dependent inner cortex. In 59% of the patients with invasive cancer but without metastases the regional lymph nodes showed weak reactive capacities (III stage). In cases with minimal metastases the immune response was dissociated (IV stage). One group of lymph nodes showed an unstimulated pattern and others a high stimulated pattern.

Topics: Acid Phosphatase; Adult; Aged; Cell Transformation, Neoplastic; Female; Glucuronidase; Humans; Lymph Nodes; Lymphatic Metastasis; Lymphocyte Activation; Lymphocytes; Middle Aged; Neoplasm Invasiveness; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Cervix Uteri; Female; Humans; Methods; Sodium Fluoride; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Carcinoma; Female; Humans; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Female; Humans; Uterine Cervical Neoplasms; Vagina

Topics: Acid Phosphatase; Adenosine Triphosphatases; Blood; Blood Protein Electrophoresis; Blood Proteins; Extracorporeal Circulation; Female; Humans; Immunoglobulin A; Immunoglobulin G; In Vitro Techniques; Isoenzymes; Radiation Effects; Radiotherapy; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Adult; Aged; Carcinoma in Situ; Carcinoma, Squamous Cell; Female; Histocytochemistry; Humans; Middle Aged; Uterine Cervical Neoplasms; Vaginal Smears

Topics: Acid Phosphatase; Biopsy; Carcinoma, Squamous Cell; Cervix Uteri; Epithelial Cells; Female; Histiocytes; Histocytochemistry; Humans; Lysosomes; Microscopy, Electron; Necrosis; Organoids; Phagocytosis; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Carcinoma, Squamous Cell; Cervix Uteri; Esterases; Female; Glycerolphosphate Dehydrogenase; Histocytochemistry; Humans; Middle Aged; Uterine Cervical Neoplasms; Uterine Cervicitis

Topics: Acid Phosphatase; Adenosine Triphosphatases; Carcinoma; Carcinoma, Squamous Cell; DNA; Epithelium; Esterases; Female; Glycogen; Glycosaminoglycans; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Precancerous Conditions; Pregnancy; RNA; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Cell Division; Cell Line; Culture Techniques; Female; HeLa Cells; Histocytochemistry; Humans; Methods; Suspensions; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Aneuploidy; Animals; Cell Line; Cell Nucleolus; Cell Nucleus; Culture Techniques; Cytoplasm; Dactinomycin; Diploidy; Female; Fetus; HeLa Cells; Histocytochemistry; Hot Temperature; Humans; Hydrogen-Ion Concentration; Lung; Staining and Labeling; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Alanine Transaminase; Alkaline Phosphatase; Aspartate Aminotransferases; Female; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Prognosis; Uterine Cervical Neoplasms; Vagina; Vaginal Diseases

Topics: Acid Phosphatase; Adenocarcinoma; Alkaline Phosphatase; Aminopeptidases; Carcinoma; Clinical Enzyme Tests; Esterases; Female; Glucosephosphate Dehydrogenase; Glutamate Dehydrogenase; Glycerolphosphate Dehydrogenase; Histocytochemistry; Histological Techniques; Humans; Hydrolases; Hydroxybutyrate Dehydrogenase; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; Malate Dehydrogenase; Oxidoreductases; Succinate Dehydrogenase; Uterine Cervical Neoplasms; Uterine Neoplasms

Topics: Abscess; Acid Phosphatase; Aged; Alkaline Phosphatase; Eosinophilia; Esterases; Female; Humans; Male; Middle Aged; Oxidoreductases; Pelvic Neoplasms; Proctitis; Prospective Studies; Radiotherapy; Radiotherapy Dosage; Rectal Neoplasms; Rectum; Urinary Bladder Neoplasms; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Aminopeptidases; Carcinoma, Squamous Cell; Cervix Uteri; Esterases; Female; Glucosephosphate Dehydrogenase; Glucuronidase; Glutamate Dehydrogenase; Histocytochemistry; Humans; Hydroxybutyrate Dehydrogenase; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; Monoamine Oxidase; Oxidoreductases; Phosphogluconate Dehydrogenase; Succinate Dehydrogenase; Uterine Cervical Neoplasms; Uterine Cervicitis

Topics: Acid Phosphatase; Carcinoma; Esterases; Female; Histological Techniques; Humans; Leucyl Aminopeptidase; Mononuclear Phagocyte System; Naphthalenes; Parathion; Staining and Labeling; Sulfhydryl Compounds; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Adenocarcinoma; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Classification; DNA, Neoplasm; Female; Fibrosarcoma; Genetics; Liver Neoplasms; Mammary Neoplasms, Experimental; Methylcholanthrene; Mice; Neoplasms, Experimental; Ribonucleases; RNA, Neoplasm; Sarcoma, Experimental; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Carcinoma, Squamous Cell; Esterases; Female; Glucosephosphate Dehydrogenase; L-Lactate Dehydrogenase; Mice; Neoplasm Transplantation; Nucleotidases; Succinate Dehydrogenase; Uterine Cervical Neoplasms; Vaginal Neoplasms

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Carcinoma, Squamous Cell; Cervix Uteri; Cobalt Isotopes; Electron Transport Complex IV; Female; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Middle Aged; Monoamine Oxidase; Succinate Dehydrogenase; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Adult; Deoxyribonucleases; Esterases; Female; Histocytochemistry; Humans; In Vitro Techniques; Middle Aged; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Aminopeptidases; Animals; Carcinoma; Carcinoma, Squamous Cell; Enzymes; Esterases; Female; Glucosephosphate Dehydrogenase; Glucuronidase; Glutamate Dehydrogenase; Glycerolphosphate Dehydrogenase; Histocytochemistry; Humans; Hydro-Lyases; Hydroxybutyrate Dehydrogenase; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; Malate Dehydrogenase; Methylcholanthrene; Mice; Neoplasms, Experimental; Research; Succinate Dehydrogenase; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Cobalt Isotopes; Female; Genitalia, Female; Humans; Radiotherapy; Uterine Cervical Neoplasms

Topics: 5'-Nucleotidase; Acid Phosphatase; Adenosine Triphosphate; Alkaline Phosphatase; Antineoplastic Agents; Biomedical Research; Biopsy; Female; Histocytochemistry; Humans; Nucleic Acids; Nucleotidases; Protein Tyrosine Phosphatases; Triaziquone; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Deoxyribonucleases; DNA; DNA, Neoplasm; Female; Histocytochemistry; Humans; Neoplasms; Uterine Cervical Neoplasms

Topics: 5'-Nucleotidase; Acid Phosphatase; Female; Humans; Nucleotidases; Protein Tyrosine Phosphatases; Uterine Cervical Neoplasms; Uterine Cervicitis

Topics: Acid Phosphatase; Alkaline Phosphatase; Carboxylesterase; Coloring Agents; Epithelium; Esterases; Female; Histocytochemistry; Humans; Phosphoric Monoester Hydrolases; Uterine Cervical Neoplasms

Topics: Acid Phosphatase; Body Fluids; Clinical Laboratory Techniques; Female; Glucuronidase; Humans; Male; Phosphoric Monoester Hydrolases; Prostatic Neoplasms; Protein Tyrosine Phosphatases; Uterine Cervical Neoplasms