acid-phosphatase and Typhoid-Fever

Various chemicals, including some bacteria-derived components, modulate natural killer cell (NKC) activity. We have analyzed the effect of wild-type Ty2 and of mutant strain TYT1231 Salmonella typhi-infected monocytes (U937 cells and human autologous monocytes) on NKC cytotoxicity of peripheral blood mononuclear cell (PBMC) and highly purified NKC (HPNKC; CD16+/56+ > 95%; the rest corresponding to CD3+ T-cells). PBMC's co-culture with either S. typhi strain infected U937 cells (medium or non-infected U937 cells as controls) resulted in the induction of lymphocyte activated killer (LAK) cell activity showing cytotoxicity against target human NKC-resistant lymphoblastoid Daudi cell line. Comparable experiments using autologous monocytes gave similar results. Co-culture of HPNKC preparations with either S. typhi strain infected U937 cells resulted in increased LAK cell activity against target Daudi cells in each and everyone of the five samples tested; paired Student's t-test p < 0.01 for both times (20 and 40 h) tested. Similar to the results observed in the experiments using PBMC, we did not find significant differences in the ability between medium and non-infected cells, or between wild-type S. typhi Ty2 and mutant strain TYT1231 infected U937 cells, to induce LAK activity in HPNKC preparations. PBMC co-incubation with either S. typhi strain infected U937 cells or autologous monocytes resulted in significant increases in IL-12, TNF-alpha, and IFN-gamma secretion. In similar experiments using HPNKC samples instead, infected U937 cells significantly increased IL-12 and IFN-gamma, but not TNF-alpha secretion. PBMC co-incubation with non-infected U937 cells, but not with non-infected monocytes, significantly increased supernatant IL-12 and TNF-alpha levels (no significant changes in IFN-gamma were recorded). Secreted cytokines remained essentially unchanged after co-incubating HPNKC preparation with non-infected U-937 cells. Incubation of PBMC or HPNKC preparations with either S. typhi strain infected U937 cells failed to produce significant changes in the expression of NKC lineage (CD16+/56+) or activation (CD28+, CD69+ and CD95+) markers. The ability of infected monocytes to induce LAK activity, release NKC cytokines and upmodulate NKC's CD95+ marker expression was essentially the same for both infecting Salmonella strains used. These results suggest a role for NKC in the physiological defensive response against intracellularly infected monocytes represent

Topics: Acid Phosphatase; Adult; Antibody-Dependent Cell Cytotoxicity; Cell Separation; Cell Survival; Coculture Techniques; Female; Humans; Interferon-gamma; Interleukin-12; Killer Cells, Natural; Male; Middle Aged; Monocytes; Phenotype; Tumor Necrosis Factor-alpha; Typhoid Fever

Cytochemistry and cytofluorometry were employed to study the main components of the intraleukocyte microbicide system of neutrophils in 52 patients with typhoid fever. During the disease there was a consistent decrease in the content of cationic protein and in the activity of leukocyte myeloperoxidase depending on the stage and severity of the pathological process. At the same time an increase in the glycogen content and activation of acid and alkaline phosphatases of leukocytes with a maximum at the disease height were detected. Addition of the complications aggravated cytochemical alterations in the cell and led to the delay in their elimination. Profound qualitative and quantitative changes in the activity of intracellular components with a visible clinical recovery of the patients pointed to an unfavourable prognosis.

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Alkaline Phosphatase; Blood Proteins; Female; Glycogen; Humans; Male; Middle Aged; Neutrophils; Peroxidase; Typhoid Fever

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Humans; Middle Aged; Neutrophils; Peroxidase; Typhoid Fever

Topics: Acid Phosphatase; Clinical Enzyme Tests; Glycerolphosphate Dehydrogenase; Humans; Lymphocytes; Prognosis; Succinate Dehydrogenase; Typhoid Fever

Topics: Acid Phosphatase; Adult; Aged; Carrier State; Chronic Disease; Glycerolphosphate Dehydrogenase; Humans; Lymphocytes; Middle Aged; Succinate Dehydrogenase; Typhoid Fever

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Carrier State; Chronic Disease; Enzyme Activation; Female; Humans; Male; Middle Aged; Neutrophils; Peroxidase; Typhoid Fever

Topics: Acid Phosphatase; Alkaline Phosphatase; Humans; Typhoid Fever

Topics: Acid Phosphatase; Animals; Clinical Enzyme Tests; Immunization; Leukocytes; Mice; NADPH Dehydrogenase; Prognosis; Time Factors; Typhoid Fever

Topics: Acid Phosphatase; Alkaline Phosphatase; Aminopeptidases; Animals; Bone Marrow; Bone Marrow Cells; Cathepsins; Esterases; Guinea Pigs; Hydrolases; Lipase; Lipoprotein Lipase; Lysosomes; Macrophages; Typhoid Fever

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Carrier State; Humans; Middle Aged; Neutrophils; Phagocytosis; Typhoid Fever

Topics: Acid Phosphatase; Animals; Cathepsins; Electron Transport Complex II; Glucuronidase; Immunity; Metabolism; Mice; Muramidase; Peritoneum; Pharmacology; Research; Succinate Dehydrogenase; Typhoid Fever; Typhoid-Paratyphoid Vaccines