acid-phosphatase and Tuberculosis--Pulmonary

Mycobacterium bovis bacille Calmette-Guerin (BCG) provides only limited protection against pulmonary tuberculosis. We tested the hypothesis that BCG might have retained immunomodulatory properties from its pathogenic parent that limit its protective immunogenicity. Mutation of the molecules involved in immunomodulation might then improve its vaccine potential. We studied the vaccine potential of BCG mutants deficient in the secreted acid phosphatase, SapM, or in the capping of the immunomodulatory ManLAM cell wall component with α-1,2-oligomannoside. Both systemic and intratracheal challenge of mice with Mycobacterium tuberculosis following vaccination showed that the SapM mutant, compared to the parental BCG vaccine, provided better protection: it led to longer-term survival. Persistence of the SapM-mutated BCG in vivo resembled that of the parental BCG indicating that this mutation will likely not compromise the safety of the BCG vaccine. The SapM mutant BCG vaccine was more effective than the parental vaccine in inducing recruitment and activation of CD11c(+) MHC-II(int) CD40(int) dendritic cells (DCs) to the draining lymph nodes. Thus, SapM acts by inhibiting recruitment of DCs and their activation at the site of vaccination.

Topics: Acid Phosphatase; Animals; Bacterial Proteins; BCG Vaccine; Cytokines; Female; Humans; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mycobacterium bovis; Mycobacterium tuberculosis; Sequence Deletion; Tuberculosis, Pulmonary

Tuberculosis Research Centre, Indian Council of Medical Research, Madras, India.. To elucidate the role of HLA-Class II genes/gene products on phagocyte enzymes such as lysozyme, beta-glucuronidase and acid phosphatase in the plasma of pulmonary tuberculosis patients.. Serological determination of HLA-DR and -DQ antigens was carried out in 54 active and 84 inactive pulmonary tuberculosis (quiescent) patients and 36 healthy control subjects. The levels of lysozyme, beta-glucuronidase and acid phosphatase were measured in the plasma of tuberculosis patients and control subjects.. Increased lysozyme levels were observed in active pulmonary tuberculosis patients. beta-glucuronidase activity was higher in inactive-TB than in active-TB patients and control subjects. HLA-DR2 positive patients showed a lower lysozyme level than -DR2 negative patients.. Increase in the plasma lysozyme level in active TB reveals the active stage of the disease. Further, increase in the activity of beta-glucuronidase in inactive-TB patients reveals the quiescent stage of the disease. The low level of lysozyme in HLA-DR2 positive patients may also be one of the possible factors involved in susceptibility to tuberculosis.

Topics: Acid Phosphatase; Adult; Female; Glucuronidase; Histocompatibility Testing; HLA-DR2 Antigen; Humans; Male; Middle Aged; Muramidase; Phenotype; Prognosis; Tuberculosis, Pulmonary

Bronchoalveolar lavage (BALF) was found to be a useful index of cell damage. It has been observed that the enzymes in BALF could give an idea of cell damage in a pulmonary disease. Acid phosphatase, alkaline phosphatase, leucine aminopeptidase and gamma-glutamyl-transpeptidase were assessed in mild, moderate, and severe pulmonary tuberculosis patients and Mantoux-negative normal controls. Activities of these enzymes were found to be higher in patients and were increasing with the severity of the disease. Increase in these enzymes in pulmonary tuberculosis patients could be attributed to lung tissue damage.

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Bronchoalveolar Lavage Fluid; Female; gamma-Glutamyltransferase; Humans; Leucyl Aminopeptidase; Male; Middle Aged; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Alkaline Phosphatase; Humans; Immunologic Techniques; Leukocytes; Peroxidases; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Alkaline Phosphatase; Humans; Lymphocytes; NAD+ Nucleosidase; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Adult; Aged; B-Lymphocytes; Glucuronidase; Humans; Lymphocytes; Male; Middle Aged; T-Lymphocytes; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Alkaline Phosphatase; Humans; Neutrophils; Prognosis; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Adolescent; Alkaline Phosphatase; Child; Clinical Enzyme Tests; Enzyme Activation; Histocytochemistry; Humans; Leukocytes; Peroxidases; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Ceruloplasmin; Enzyme Activation; Female; Humans; Lung Diseases; Male; Middle Aged; Succinate Dehydrogenase; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Female; Humans; Leukocytes; Male; Middle Aged; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Adolescent; Alkaline Phosphatase; Child; Child, Preschool; Glycogen; Humans; Infant; Leukocytes; Peroxidases; Phospholipids; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Bronchi; Bronchial Fistula; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Postoperative Complications; Proteins; RNA; Succinate Dehydrogenase; Sulfhydryl Compounds; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Acute Disease; Alkaline Phosphatase; Bacterial Infections; Carbon Isotopes; Citric Acid Cycle; Glucose; Glucosephosphate Dehydrogenase; Glutathione Reductase; Humans; Lupus Erythematosus, Systemic; Neoplasms; Neutrophils; Osteomyelitis; Oxygen Consumption; Peroxidases; Phosphogluconate Dehydrogenase; Pyruvate Kinase; Tetrazolium Salts; Tuberculosis, Pulmonary; Virus Diseases

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Aorta; Arteriosclerosis; Blood Vessels; Child; Chronic Disease; Coronary Disease; Coronary Vessels; Esterases; Female; Humans; Hyperthyroidism; Lipase; Lipid Metabolism; Liver Cirrhosis; Male; Middle Aged; Pneumonia; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Isoniazid; Lung; Lymphoid Tissue; Macrophages; Phagocytosis; Rabbits; Succinate Dehydrogenase; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Child; Diabetes Complications; Diabetes Mellitus; Dihydrolipoamide Dehydrogenase; Female; Glycosaminoglycans; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Lipid Metabolism; Lung; Male; Middle Aged; Oxidoreductases; Phosphoric Monoester Hydrolases; Succinate Dehydrogenase; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Animals; Cell Count; Deoxyribonucleases; Esterases; Female; Glucuronidase; Lipase; Macrophages; Male; Muramidase; Pulmonary Alveoli; Rabbits; Radiation Injuries, Experimental; Ribonucleases; Tuberculosis; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Alkaline Phosphatase; Caseins; Female; Granulation Tissue; Humans; Leukocytes; Male; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Humans; Methods; Neutrophils; Spectrophotometry; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Glycosaminoglycans; Histocytochemistry; Immunity; Lung; Lymph Nodes; Methods; Polysaccharides; Rats; RNA; Time Factors; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; DNA; Guinea Pigs; Histocytochemistry; Lung; Methods; Microscopy, Electron; Mycobacterium tuberculosis; Pulmonary Alveoli; RNA; Succinate Dehydrogenase; Tuberculosis, Pulmonary; Virulence

Topics: Acid Phosphatase; Amides; Animals; Antitubercular Agents; Culture Media; Drug Resistance, Microbial; Esterases; Ghana; Guinea Pigs; Humans; Methods; Mycobacterium; Mycobacterium bovis; Mycobacterium tuberculosis; Nicotinic Acids; Oxidoreductases; Rabbits; Sputum; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Acute Disease; Adolescent; Adult; Aged; Female; Histocytochemistry; Humans; Male; Middle Aged; Myocardial Infarction; Neutrophils; Pneumonia; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Animals; Body Weight; Cell Wall; Histocytochemistry; Hydrogen-Ion Concentration; Lung; Lysosomes; Male; Mice; Mycobacterium bovis; Mycobacterium tuberculosis; Phagocytosis; Sodium Hydroxide; Surface-Active Agents; Tissue Extracts; Trypsin; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Alkaline Phosphatase; Female; Humans; Leukocytes; Lipids; Middle Aged; Neutrophils; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Alcoholism; Alkaline Phosphatase; Animals; Antitubercular Agents; Bronchi; Ethanol; Guinea Pigs; Histocytochemistry; Humans; Isoniazid; Streptomycin; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Alkaline Phosphatase; Bronchiectasis; Diagnosis, Differential; Humans; Lung Abscess; Lung Neoplasms; Neoplasms; Serum; Tuberculosis; Tuberculosis, Pulmonary

Topics: Acid Phosphatase; Phosphoric Monoester Hydrolases; Research; Silicosis; Tuberculosis; Tuberculosis, Pulmonary