acid-phosphatase and Tongue-Neoplasms

Green tea polyphenols (GTP) has been used as a chemopreventive agent world wide against chemically induced cancer. The present study is aimed to understand the therapeutic action of GTP on glycoconjugates and immunological markers in 4-Nitroquinoline 1-oxide (4-NQO)-induced oral cancer over a period of 30 days at 200mg/kg, p.o., Oral cancer was induced by painting 4-NQO for 8 weeks followed by administration of GTP after 22 weeks, for 30 days. Glycoconjugates such as hexose, hexosamine, sialicacid, fucose and mucoprotein were analysed. Expression of glycoconjugates was examined through histology and SDS-PAGE. Immunological markers such as circulating immune complex and mast cell density were studied. Oral cancer-induced animals showed a significant increase in levels of glycoconjugates and its expression, similar to that observed for immunological markers. Treatment with GTP altered the expression of glycoconjugates as well as immunological markers. The results suggest that GTP modulates both the expression of glycoconjugates and immunological markers resulting in regression of oral cancer.

Topics: 4-Nitroquinoline-1-oxide; Acid Phosphatase; Animals; Antineoplastic Agents, Phytogenic; beta-Galactosidase; Camellia sinensis; Flavonoids; Glucuronidase; Glycoconjugates; Hexoses; Immunoglobulins; Male; Mouth; Mouth Neoplasms; Mucoproteins; N-Acetylneuraminic Acid; Phenols; Phospholipases; Phytotherapy; Plant Extracts; Polyphenols; Rats; Rats, Wistar; Tongue; Tongue Neoplasms

This study evaluated the ability of bisphosphonate to prevent bone resorption induced by metastatic tumor cells.. Autopsy specimens of a bone metastasis from a woman with a primary squamous cell carcinoma of the tongue who developed multiple osteolytic lesions and hypercalcemia and was treated with pamidronate were studied histologically, histochemically, and ultrastructurally. In an animal experiment, cultured tumor cells (1 x 10(5)) obtained from a metastatic submandibular lymph node in the same patient were injected in the left ventricle of nude mice, and a resulting metastatic bone lesion was studied histologically and histochemically.. In the autopsy specimens, despite the presence of many resorption lacunae on bone surface, only a few small tartrate-resistant acid phosphatase (TRAPase)-positive cells were observed, and most of them were stained weakly and detached from the bone surface. In the animal experiment, 1 of 10 animals (10%) formed osteolytic bone metastasis, and many TRAPase-positive cells were observed histochemically.. Biphosphonate inhibits bone resorption induced by tumor, possibly by decreasing the number of osteoclasts and inhibiting their function.

Topics: Acid Phosphatase; Aged; Animals; Bone Neoplasms; Bone Resorption; Carcinoma, Squamous Cell; Diphosphonates; Female; Humans; Hypercalcemia; Isoenzymes; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Osteoclasts; Pamidronate; Spinal Neoplasms; Tartrate-Resistant Acid Phosphatase; Thoracic Vertebrae; Tongue Neoplasms; Tumor Cells, Cultured

Topics: Acid Phosphatase; Alkaline Phosphatase; Anus Neoplasms; Carcinoma, Squamous Cell; Cheek; Female; Histocytochemistry; Humans; Male; Mouth Neoplasms; Penile Neoplasms; Tongue Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Anus Neoplasms; Carcinoma, Squamous Cell; Cheek; Facial Neoplasms; Histocytochemistry; Humans; Male; Penile Neoplasms; Tongue Neoplasms

Seven typical granular cell myoblastomas, 4 from the skin (2 multicentric) and 1 each from the tongue, vulva and breast, were studied with the electron microscope and with cytochemical procedures for the visualization of lysosomes, endoplasmic reticulum, mitochondria, Golgi apparatus and membranes. With these parameters, all of the lesions were found to be virtually identical. To the authors' knowledge, the apparent formation of the specific small granule from the Golgi apparatus and the large granule (cytolysome) by segregation of portions of cell cytoplasm as well as the apparent aging process in myoblastoma cells is described for the first time. The small granules resemble lysosomes, but do not stain with the lysosomal markers employed. The large granules (cytolysomes) contain acid phosphatase but only a few contain thiolacetic acid esterase activity, suggesting that there are at least two varieties of cytolysomes in myoblastoma. It is concluded that myoblastoma is a tumor-like lesion of Schwann cell origin, which is either a reactive cellular response or, more likely, a true neoplasm.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adult; Aging; Alcohol Oxidoreductases; Alkaline Phosphatase; Breast Neoplasms; Child; Endoplasmic Reticulum; Female; Golgi Apparatus; Histocytochemistry; Humans; Lysosomes; Male; Methods; Microscopy, Electron; Middle Aged; Mitochondria; Neoplasms, Muscle Tissue; Phosphoric Monoester Hydrolases; Schwann Cells; Skin Neoplasms; Tongue Neoplasms; Vulvar Neoplasms

Topics: Acid Phosphatase; Adenocarcinoma; Biopsy; Carcinoma, Bronchogenic; Carcinoma, Squamous Cell; Colorimetry; Esophageal Neoplasms; Female; Gastrointestinal Neoplasms; Hodgkin Disease; Humans; Intestinal Neoplasms; Laryngeal Neoplasms; Leucyl Aminopeptidase; Leukemia; Liver Neoplasms; Lymphoma, Non-Hodgkin; Male; Melanoma; Nasopharyngeal Neoplasms; Neoplasms; Pancreatic Neoplasms; Prostatic Neoplasms; Tongue Neoplasms; Urogenital Neoplasms

Topics: Acid Phosphatase; Adenoma; Adenosine Triphosphatases; Histocytochemistry; Humans; Male; Middle Aged; Salivary Glands; Tongue Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Carcinoma, Squamous Cell; Choriocarcinoma; Duodenal Neoplasms; Esophageal Neoplasms; Female; Humans; Liver Neoplasms; Lung Neoplasms; Neoplasm Metastasis; Neoplasms; Pregnancy; Stomach Neoplasms; Tongue Neoplasms