acid-phosphatase and Streptococcal-Infections

Endothelial dysfunction is a major component of the pathophysiology of septicaemic group B Streptococcus (GBS) infections. Although cytokines have been shown to activate human umbilical vein endothelial cells (HUVECs), the capacity of interferon (IFN)-γ to enhance the microbicidal activity of HUVECs against GBS has not been studied. We report that the viability of intracellular bacteria was reduced in HUVECs activated by IFN-γ. Enhanced fusion of lysosomes with bacteria-containing vacuoles was observed by acid phosphatase and the colocalisation of Rab-5, Rab-7 and lysosomal-associated membrane protein-1 with GBS in IFN-γ-activated HUVECs. IFN-γ resulted in an enhancement of the phagosome maturation process in HUVECs, improving the capacity to control the intracellular survival of GBS.

Topics: Acid Phosphatase; Anti-Infective Agents; Bacterial Adhesion; Endocytosis; Human Umbilical Vein Endothelial Cells; Humans; Interferon-gamma; Lysosomes; Microbial Viability; Phagosomes; Primary Cell Culture; Streptococcal Infections; Streptococcus agalactiae; Survival Analysis

The phagocytosis of two type 12 Streptococcus pyogenes strains differing in M protein presence was investigated in vivo. The rate of phagocytosis and the streptococcal viability in phagocytic cells and outside of them was studied simultanously with estimation of enzymatic activity in phagocytic cells. It was observed that M positive strain was phagocytized at lower rate and also exhibited longer viability. Decrease of lactic dehydrogenase and ATP-ase activity in phagocytes of rabbits infected with M+ strain was also detected. These results suggest that M protein disturbs phagocytosis by inhibiting their main metabolic pathway of glycolysis.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Animals; Ascitic Fluid; Bacterial Proteins; Glucuronidase; L-Lactate Dehydrogenase; Macrophages; Male; Mutation; Phagocytes; Phagocytosis; Rabbits; Streptococcal Infections; Streptococcus pyogenes; Succinate Dehydrogenase

Plasma membranes were isolated from the livers of control and Streptococcus pneumoniae-infected rats. This work, therefore, represents the first isolation of plasma membranes from infected actron microscopy and by the use of enzyme markers for microsomes (glucose-6-phosphatase), mitochondria (glutamate and malate dehydrogenases), and lysosomes (acid phosphatase). Plasma membranes from infected cells banded at the same sucrose density as plasma membranes from uninfected cells. Moreover, equivalent amounts of plasma membranes could be isolated from control and infected rat livers. There were, however, significant alterations in the enzyme complement of the plasma membrane after infection. 5'-Nucleotidase activity was significantly decreased, whereas alkaline phosphatase activity was significantly increased. Kinetic analysis demonstrated that only the Vmax and not the Km of these two enzymes was changed, suggesting that the altered affinity of the enzymes for substrate was not the mechanism responsible for the observed alterations. No change in the mitochondrial enzyme markers was observed after infection, but the specific activity of microsomal glucose-6-phosphatase decreased significantly. Possible explanations for the observed alterations are discussed.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Cell Membrane; Glucose-6-Phosphatase; Hydrogen-Ion Concentration; Liver; Male; Microsomes, Liver; Mitochondria, Liver; Nucleotidases; Rats; Streptococcal Infections; Streptococcus pneumoniae

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Escherichia coli Infections; Lymphocyte Activation; Lymphocytes; Macrophages; Mice; Phagocytosis; Staphylococcal Infections; Streptococcal Infections; Uracil

Topics: Acid Phosphatase; Adult; Agranulocytosis; Alkaline Phosphatase; Bacterial Infections; Chediak-Higashi Syndrome; Chemotaxis; Female; Glucuronidase; Humans; Leukocytes; Lysosomes; Male; Muramidase; Peroxidases; Skin Window Technique; Staphylococcal Infections; Streptococcal Infections

Topics: Acid Phosphatase; Agglutination Tests; Antibodies; Antigen-Antibody Complex; Arthus Reaction; Complement Fixation Tests; Complement System Proteins; Humans; Immune Adherence Reaction; Neutrophils; Peptide Hydrolases; Pharynx; Respiratory Tract Infections; Staphylococcal Infections; Streptococcal Infections; Streptococcus pyogenes; Vascular Diseases

Topics: Acid Phosphatase; Animals; Arthritis, Infectious; Endoplasmic Reticulum; Golgi Apparatus; Histocytochemistry; Microscopy, Electron; Nucleoproteins; Organoids; Streptococcal Infections; Swine; Synovial Membrane