acid-phosphatase and Stomach-Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Brain Neoplasms; Breast Neoplasms; Carcinoma, Hepatocellular; Catalase; DNA Nucleotidyltransferases; Fructose-Bisphosphate Aldolase; Glycine Hydroxymethyltransferase; Hexokinase; Hodgkin Disease; Intestinal Neoplasms; Isoenzymes; L-Lactate Dehydrogenase; Leukemia; Liver; Liver Neoplasms; Lung Neoplasms; Neoplasms; Pyruvate Kinase; Ribonucleotides; Sarcoma, Experimental; Stomach Neoplasms; Uridine Kinase

The present study investigated the clinical significance of tartrate-resistant acid phosphatase type-5 (ACP5) expression in gastric cancer.. In 150 specimens of gastric cancer and adjacent normal mucosa, expression of ACP5 protein and mRNA and was determined by immunohistochemical staining and quantitative real-time polymerase chain reaction, respectively.. Expression of ACP5 mRNA was significantly higher in cancer tissues than in adjacent normal mucosa. Elevated ACP5 mRNA was associated with lymph node metastasis and peritoneal dissemination. Logistic regression analysis revealed that elevated ACP5 expression was an independent risk factor for peritoneal dissemination and was associated with shorter survival. Immunohistochemical staining of primary carcinomas showed ACP5 to be expressed mainly in the cytoplasm.. ACP5 is predictive of peritoneal dissemination in patients with gastric cancer, and might play a crucial role in the establishment of peritoneal dissemination.

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Aged, 80 and over; Biomarkers, Tumor; Female; Gastric Mucosa; Humans; Immunohistochemistry; Isoenzymes; Male; Middle Aged; Real-Time Polymerase Chain Reaction; RNA, Messenger; Stomach Neoplasms; Tartrate-Resistant Acid Phosphatase; Young Adult

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Carcinoma; HSP70 Heat-Shock Proteins; Male; Mice; Proliferating Cell Nuclear Antigen; Stomach Neoplasms

The distribution of ALPase, ACPase, G6Pase TPPase and CCOase of gastric cancer and normal gastric epithelium were studied ultrastructurally. The results showed that normal gastric epithelium had no ALPase reaction. The reactions of ACPase, G6Pase, TPPase and CCOase were found in the corresponding organellae which were consistent with their functions. In tubular adenocarcinoma cells, their reactions were more apparent in the corresponding organellae. Some cells of tubular adenocarcinomas showed ALPase reaction. The mucinous adenocarcinoma cells had higher ACPase and TPPase reactions. In poorly differentiated adenocarcinoma cells, the five marker enzymes showed negative or faint reactions. The biological significance and mechanisms of distribution of the five marker enzymes were discussed.

Topics: Acid Phosphatase; Adenocarcinoma; Adenocarcinoma, Mucinous; Alkaline Phosphatase; Biomarkers, Tumor; Glucose-6-Phosphatase; Histocytochemistry; Humans; Stomach Neoplasms

Topics: Acid Phosphatase; Adult; Aged; alpha-Fetoproteins; Biomarkers, Tumor; Chorionic Gonadotropin; Chorionic Gonadotropin, beta Subunit, Human; Female; Ferritins; Humans; Male; Middle Aged; Peptide Fragments; Prostate; Stomach Neoplasms

Neutrophil level in blood (1 l) and those of certain enzymes in neutrophils were measured in 36 patients with stomach tumors and 40 healthy controls. The range of the enzymes included myeloperoxidase (MPO), beta-glucuronidase (GR), N-acetyl-beta-D-glucosaminidase (NAG), acid phosphatase (AcP), alkaline phosphatase (AlP), lactate dehydrogenase (LD) and dehydrogenase-glucoso-6-phosphoric acid (DG-6-P). Glycogen and lipids were also assayed. The MPO and AcP levels of neutrophils in cancer patients were found to be significantly higher than in healthy controls, whereas the levels of the other enzymes were not. The glycogen and lipid concentrations in neutrophils in cancer patients were significantly lower than in healthy controls.

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Female; Glucosephosphate Dehydrogenase; Glucuronidase; Glycogen; Hexosaminidases; Humans; L-Lactate Dehydrogenase; Leukocyte Count; Lipids; Male; Middle Aged; Neutrophils; Peroxidase; Stomach Neoplasms

The activity of acid phosphatase, beta-glucuronidase and N-acetyl-beta-glucosaminidase was assessed using semiquantitative cytochemical methods in peripheral blood lymphocytes of 45 untreated patients with gastric cancer and 80 healthy subjects. In cancer patients the study demonstrated a statistically significant decrease in the number of lymphocytes with granular reaction for acid phosphatase and N-acetyl-beta-glucosaminidase, as well as an increase in the number of lymphocytes showing a granular-diffuse reaction for the above enzymes and a diffuse reaction for all the studied lysosomal enzymes. Possible mechanisms of the observed changes are discussed.

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Aged; Female; Glucuronidase; Histocytochemistry; Humans; Lymphocytes; Lysosomes; Male; Middle Aged; Stomach Neoplasms

We describe the ultrastructure of various types of gastric carcinoma cells as well as their histochemical properties as visualized at the electron-microscope level. The histochemical properties of tumour cells were compared with those of homologous normal epithelial cells. The localization and activity of ATPase, IDPase, acidic phosphatase and alkaline phosphatase as well as of the oxidoreductases (cytochrome oxidase, succinate dehydrogenase and NADH-dehydrogenase) were studied. Our findings demonstrated that, in tumour cells, a complicated process of structural-functional restructuring takes place. It seems that a number of ultracytochemical properties may be preserved or may disappear altogether; also, such properties may become enhanced or weaker. This heterogeneity of the histochemical properties of tumour cells is discussed with regard to the role of the stem (polypotent) cell in the process of the histogenesis (cytogenesis) of human gastric carcinomas.

Topics: Acid Anhydride Hydrolases; Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Carcinoma; Cell Differentiation; Duodenum; Gastric Mucosa; Histocytochemistry; Humans; Intestinal Mucosa; Oxidoreductases; Phosphoric Monoester Hydrolases; Stomach Neoplasms

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Aged; Clinical Enzyme Tests; Female; Glucuronidase; Humans; Lymphocytes; Lysosomes; Male; Middle Aged; Stomach Neoplasms

In 132 patients suffering from various malignancies, including uterine carcinoma, breast cancer, gastric carcinoma, cancer of the colon, and additionally, patients with uterine myomas and endometriosis an increased activity of AP within the peripheral blood neutrophils could be stated by using a histochemical method. According to the author's opinion an increased activity of that enzyme reflects a response against local inflammatory processes frequently accompanying malignant tumours.

Topics: Acid Phosphatase; Breast Neoplasms; Colonic Neoplasms; Endometriosis; Female; Humans; Leiomyoma; Male; Neoplasms; Neutrophils; Stomach Neoplasms; Uterine Neoplasms

In 22 patients with alimentary tract carcinoma granulocyte acid phosphatase activity was determined using the histochemical method of Suzuki. Enzyme activity was determined in peripheral blood 3 times, viz. before surgical intervention, in blood from a vessel draining the tumour before its excision, and in peripheral blood 2-3 weeks after excision of the tumour. In parallel tests, enzyme activity was established in peripheral blood of 22 healthy individuals. The results showed that acid phosphatase activity of granulocytes is lower than in the controls, especially in granulocytes collected from the vessel draining the tumour. The lower acid phosphatase activity in granulocytes from patients with carcinoma was mainly due to the lower percentage of positive cells. After removal of the tumour acid phosphatase activity of the granulocytes increased in the majority of cases. It is assumed that an enhanced exocytosis of lysosomal enzymes in the course of tumour disease was responsible for the decrease of activity in the granulocytes.

Topics: Acid Phosphatase; Humans; Neutrophils; Rectal Neoplasms; Stomach Neoplasms

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Female; Glucuronidase; Humans; Male; Middle Aged; Neutrophils; Peroxidase; Stomach Neoplasms

Microgastric biopsies were obtained from 20 patients with clinical, radiologic, and endoscopic features of gastric neoplasms. Biopsy specimens were taken from both macroscopically normal gastric mucosa (confirmed histology), and from the lesion. In 16 patients, various stages of adenocarcinoma were confirmed. Repeated biopsy examinations, ruled out malignancy in the four remaining patients, in which a final diagnosis of healing ulcers was established. Homogenates were prepared from the biopsy specimens, and subsequently assayed individually for acid- and alkaline phosphatase and acid proteases. Significant variances (P less than 0.05), were demonstrated for acid protease activities when tumor homogenate levels were compared; firstly with normal mucosa and secondly with ulcer homogenate levels. Biopsy hydrolase determinations may be valuable in providing important additional information not obtained from histologic examinations.

Topics: Acid Phosphatase; Adenocarcinoma; Alkaline Phosphatase; Biopsy; Clinical Enzyme Tests; Diagnosis, Differential; Humans; Peptic Ulcer; Peptide Hydrolases; Stomach Neoplasms

The report deals with the ultrastructural cell patterns of gastric carcinoma and electron-microscopy study of the histochemical features of cells. The histochemical features of tumor cells were compared with homologous normal epithelial cells. The localization and activity of ATP-ase, IDP-ase, acid and alkaline phosphatases were studied. The data obtained suggest a structural and functional reorganization in the cells of human stomach tumors. It was also found that the histological and ultrastructural differentiation and the degree of functional maturity of tumor cells do not necessarily coincide.

Topics: Acid Anhydride Hydrolases; Acid Phosphatase; Adenosine Triphosphatases; Biopsy; Duodenum; Gastric Mucosa; Histocytochemistry; Humans; Intestinal Mucosa; Microscopy, Electron; Phosphoric Monoester Hydrolases; Stomach Neoplasms

A prospective study compared five different assays for serum prostatic acid phosphatase in the detection of carcinoma of the prostate gland. The assays included two radioimmunoassay procedures, one counterimmunoelectrophoresis procedure, and an enzymatic procedure using alpha-naphthol phosphate substrate with and without sodium tartrate inhibition. The patients' hospital records were reviewed, as were all available surgical histology slides. The patients were divided into four groups: prostatic carcinoma, benign prostatic hypertrophy, other carcinomas (besides prostatic carcinoma), and no related disease states (that would be expected to give elevated acid phosphatase levels). The results were analyzed with respect to sensitivity, specificity, predictive value of a positive result, predictive value of a negative result, and efficiency of the assays.

Topics: Acid Phosphatase; Adenocarcinoma; Carcinoma; Colonic Neoplasms; Counterimmunoelectrophoresis; Enzyme-Linked Immunosorbent Assay; Humans; Lung Neoplasms; Male; Prospective Studies; Prostate; Prostatic Hyperplasia; Prostatic Neoplasms; Radioimmunoassay; Stomach Neoplasms

In 25 patients (10 women and 15 men) aged 37 to 68 years activity of the following enzymes has been determined by the use of semiquantitative cytochemical methods: acid and alkaline phosphatase, beta-glucuronidase, N-acetyl-beta-glucosaminidase and myeloperoxidase. Additionally the content of glycogen and lipids has been determined in the cells studied. An increase of the myeloperoxidase and acid phosphatase activity and a decrease of the glycogen and the lipid content has been stated in neutrophils of the patients. Above alterations may reflect antitumor reactivity of neutrophils or nonspecific neutrophil response against inflammatory changes accompanying the tumor area.

Topics: Acetylglucosaminidase; Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Female; Glucuronidase; Glycogen; Humans; Lipids; Male; Middle Aged; Neutrophils; Peroxidase; Stomach Neoplasms

Activity of acid phosphatase, beta-glucuronidase and N-acetyl-beta-glucosaminidase was investigated cytochemically in neutrophils from peripheral blood of 22 untreated patients with gastric cancer, 8 patients with cancer of large intestine and in 40 healthy individuals. Differences in the activity of enzymes studied were demonstrated between patients at different clinical stages of cancer advancement, as well as between cancer patients and healthy subjects. Most significant changes were observed in patients with initial (1st stage) cancer, as compared with the control group, including decrease of acid phosphatase and beta-glucuronidase activity, accompanied by an enhanced N-acetyl-beta-glucosaminidase activity. The possible mechanism of these changes is discussed.

Topics: Acetylglucosaminidase; Acid Phosphatase; Adenocarcinoma; Adult; Aged; Carcinoma; Colonic Neoplasms; Female; Glucuronidase; Humans; Lysosomes; Male; Middle Aged; Neutrophils; Stomach Neoplasms

Electron-histochemical study of phosphohydrolases (ATPase, acid and alkaline phosphatases) in cells of the normal gastric mucosa, duodenal mucosa and gastric adenocarcinoma of man was carried out. In cancer cells retaining to a certain extent the ultrastructural features of the chief cells, parietal cells of enterocytes, the distribution of the product of reaction for ATPase and acid phosphatase in nucleoli, endoplasmic reticulum membranes, intracellular cannaliculi, plasmalemma, mitochondria, the distribution of the product of reaction for ATPase and acid phosphatase in nucleoli, tural features of enterocytes, no activity of alkaline phosphatase could be demonstrated in membranes of the villi of the striated border. Alongside with the retention or disappearance of electron-histochemical features, some of them may be enhanced. Thus, the activity of acid phosphatase was increased in lysosomes of cancer cells (of the type of chief cells). So, in cancer cells of adenocarcinoma the structure-functional rearrangement going in different directions is observed in addition to the process of simplification and unification.

Topics: Acid Phosphatase; Adenocarcinoma; Adenosine Triphosphatases; Alkaline Phosphatase; Cytoplasm; Gastric Mucosa; Histocytochemistry; Humans; Microscopy, Electron; Stomach Neoplasms

For the purpose of predicting the effect of carcinostatic agents on tumor-bearing host in vitro, a sensitivity test of a drug in tumor bearers was developed. This test is based on the change in the specific activity of acid phosphatase of individual tumor and liver tissue, and the difference in them was used as an indicator of effectiveness. The value was called the "effective value." The present papar is concerned with the relationship between the effective value and effectiveness of the agents in rodents and man. The results showed that the effective value became higher with increasing concentration of Mitomycin-C incubated with tumor and liver tissues. The survival time of Ehrlich carcinoma-bearing mice, which were given Mitomycin-C intraperitoneally, was longer and increase in the dose of Mitomycin-C. In ascitic or subcutaneous form of Ehrlich carcinoma, AH-130, and Yoshida sarcoma, the effective value was parallel with the survival time of or the inhibition of tumor growth in tumor-bearing animals treated with Mitomycin-C. In 25 patients with gastric carcinoma treated with Mitomycin-C, the postoperative survival time was more closely correlated with the effective value calculated by the titers of both tumor and liver than the value of tumor alone.

Topics: Acid Phosphatase; Adult; Aged; Animals; Carcinoma, Ehrlich Tumor; Drug Evaluation; Female; Humans; Liver; Male; Mice; Middle Aged; Mitomycins; Neoplasms, Experimental; Phagocytosis; Rats; Sarcoma, Yoshida; Species Specificity; Stomach Neoplasms

The mechanism of stroma-formation and rearrangement of the stroma was studied on 221 cases of cancer of the lung, stomach and mammary gland using histological, histochemical and electron-microscopy methods of investigation. It was established that multiplying epithelial elements of teh tumour induced proliferation of histiogenic and vascular fibroblasts, activating them. Active fibroblasts play a double role: they either produce fibres and interstitial matter of the tumorous stroma, or participate in desintegration of preceding and newly formed collagenous structures. The processes of stroma-formation comply with the conventional schemes of normal fibrillogenesis and rearrangement of the interstitial tissue. It was shown that fibroblasts of the tumour stroma was capable of phagocytosis of a mature collagen. It is supposed that in the course of the tumorous growth correlation between the parenchyma and stroma is maintained, the leading role being played by the epithelium.

Topics: Acid Phosphatase; Breast Neoplasms; Collagen; Fibroblasts; Glycosaminoglycans; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Lung Neoplasms; Male; Microscopy, Electron; Stomach Neoplasms; Tropocollagen

Topics: Acid Phosphatase; Adenocarcinoma; Adenocarcinoma, Mucinous; Adenocarcinoma, Scirrhous; Adenosine Triphosphatases; Carcinoma; Dihydrolipoamide Dehydrogenase; Electron Transport Complex IV; Glucosephosphate Dehydrogenase; Glycerolphosphate Dehydrogenase; Humans; Hydrolases; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Oxidoreductases; Stomach Neoplasms; Succinate Dehydrogenase

Topics: Acid Phosphatase; Blood Protein Disorders; Carcinoembryonic Antigen; Cold Temperature; Diagnosis, Differential; Fibrinogen; Humans; Lymphatic Metastasis; Male; Middle Aged; Stomach Neoplasms; Syndrome; Thrombophlebitis

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Cell Transformation, Neoplastic; Connective Tissue; Epithelial Cells; Epithelium; Gastric Mucosa; Glycosaminoglycans; Humans; Intestinal Mucosa; Metaplasia; Microscopy, Electron; Nucleic Acids; Rats; Regeneration; Stomach Neoplasms; Time Factors

Topics: Acid Phosphatase; Adenocarcinoma; Alkaline Phosphatase; Aminopeptidases; Epithelium; Esterases; Gastric Mucins; Gastric Mucosa; Glucuronidase; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Oxidoreductases; Phosphoric Monoester Hydrolases; Stomach Neoplasms; Stomach Ulcer; Succinate Dehydrogenase

Topics: Acid Phosphatase; Adenocarcinoma; Adenocarcinoma, Scirrhous; Humans; Lipid Metabolism; Neoplasm Proteins; RNA, Neoplasm; Stomach; Stomach Neoplasms

Topics: Acid Phosphatase; Adenocarcinoma; Adenocarcinoma, Mucinous; Adenocarcinoma, Scirrhous; Alkaline Phosphatase; Aminopeptidases; Cytodiagnosis; Gastric Mucosa; Glycosaminoglycans; Humans; Stomach Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Breast Neoplasms; Clinical Enzyme Tests; Diagnosis, Differential; Esophageal Neoplasms; Hodgkin Disease; Humans; Leukocytes; Lung Neoplasms; Lymphoma, Large B-Cell, Diffuse; Rectal Neoplasms; Stomach Neoplasms

Topics: Acid Phosphatase; Adult; Aged; Animals; Antineoplastic Agents; Chromomycins; Cyclophosphamide; Female; Fluorouracil; Humans; Male; Mechlorethamine; Methotrexate; Mice; Middle Aged; Mitomycins; Neoplasms; Neoplasms, Experimental; Rats; Stomach Neoplasms; Vincristine

Topics: Acetylcholinesterase; Acid Phosphatase; Adult; Aged; Alanine Transaminase; Alkaline Phosphatase; Amylases; Aspartate Aminotransferases; Cholinesterases; Diagnosis, Differential; Duodenal Ulcer; Humans; Hydrolases; Middle Aged; Stomach Neoplasms; Stomach Ulcer; Transaminases

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Animals; Antibody Formation; Antigens; Cholelithiasis; Female; Glucuronidase; Hexosaminidases; Histocytochemistry; Humans; Lupus Erythematosus, Systemic; Lymph Nodes; Lymphocytes; Male; Peroxidases; Plasma Cells; Rabbits; Rats; Sarcoidosis; Stomach Neoplasms; Stomach Ulcer; Tuberculosis

Topics: Acid Phosphatase; Alkaline Phosphatase; Bilirubin; Colonic Neoplasms; Esophageal Neoplasms; Humans; Kidney Neoplasms; Leukemia, Myeloid; Liver Neoplasms; Lung Neoplasms; Lymphoma, Large B-Cell, Diffuse; Male; Neoplasms; Pancreatic Neoplasms; Phosphatidylcholines; Phosphatidylethanolamines; Phosphatidylinositols; Phospholipids; Prostatic Neoplasms; Rectal Neoplasms; Sphingolipids; Stomach Neoplasms; Thyroid Neoplasms; Triglycerides; Urinary Bladder Neoplasms

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Bone Neoplasms; Histocytochemistry; Humans; Middle Aged; Radiation Injuries; Radiodermatitis; Radiotherapy; Skin; Stomach Neoplasms

Topics: ABO Blood-Group System; Acid Phosphatase; Adult; Alkaline Phosphatase; Blood Group Antigens; Brazil; Breast Neoplasms; Child; Diet; Electrophoresis; Female; Genetics, Medical; Hawaii; Humans; Intestines; Isoenzymes; Lewis Blood Group Antigens; Liver Cirrhosis; Lymphoma, Follicular; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Male; Phosphoric Monoester Hydrolases; Placenta; Pregnancy; Saliva; Stomach Neoplasms; Sweden; Tartrates

Topics: Acid Phosphatase; Blood Platelets; Breast Neoplasms; Buffers; Citrates; Cystic Fibrosis; Female; Heart Failure; Humans; Hydrogen-Ion Concentration; Kidney Failure, Chronic; Male; Phosphates; Prostate; Prostatic Diseases; Prostatic Neoplasms; Spectrophotometry; Stomach Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Carcinoma, Squamous Cell; Choriocarcinoma; Duodenal Neoplasms; Esophageal Neoplasms; Female; Humans; Liver Neoplasms; Lung Neoplasms; Neoplasm Metastasis; Neoplasms; Pregnancy; Stomach Neoplasms; Tongue Neoplasms

Topics: Acid Phosphatase; Diagnosis, Differential; Gastric Acidity Determination; Gastric Juice; Humans; Staining and Labeling; Stomach Diseases; Stomach Neoplasms

Topics: Acid Phosphatase; Alanine Transaminase; Alkaline Phosphatase; Amylases; Aspartate Aminotransferases; Breast Neoplasms; Clinical Enzyme Tests; Colonic Neoplasms; D-Alanine Transaminase; Humans; L-Lactate Dehydrogenase; Lipase; Lung Neoplasms; Male; Pancreatic Neoplasms; Stomach Neoplasms; Testicular Neoplasms

Topics: Acid Phosphatase; Phosphoric Monoester Hydrolases; Stomach Neoplasms

Topics: Acid Phosphatase; Biochemical Phenomena; Phosphoric Monoester Hydrolases; Stomach Neoplasms

Topics: Acid Phosphatase; Fasting; Gastric Juice; Neoplasms; Phosphoric Monoester Hydrolases; Stomach; Stomach Neoplasms