acid-phosphatase and Sarcoma--Ewing

In osteoclastic giant cells of six different tumors of bones and joints (fibrous dysplasia, proliferating giant cell tumor, malignant giant cell tumor, osteosarcoma after chemotherapy, malignant synovioma and Ewing's sarcoma) activities of tartrate-resistant acid phosphatase, NADH-tetrazolium-oxidoreductase and, in three of them, of non-specific esterase are determined by enzyme histochemical methods. Quantitative microphotometry makes it possible to determine relative enzyme activities in the cut sections of giant cells of different sizes. Giant cells of the various tumors reveal similar trends: With an increase in cell size, mean extinctions of NADH-tetrazolium-oxidoreductase and non-specific esterase decrease. Mean extinctions of tartrate-resistant acid phosphatase increase in cells of medium size, whereas the large cells reveal in part low activities. An additional ultrastructural examination of the giant cells in the proliferating giant cell tumor as well as in the osteosarcoma shows morphological signs of degeneration in the large cells. Electron probe microanalysis of the proliferating giant cell tumor exhibits evidence of phagocytosis of Ca and/or Fe containing particles. The similar size dependent reaction pattern of enzymes in osteoclastic giant cells of different tumors favors the concept of a common histogenesis, i.e. a host reaction.

Topics: Acid Phosphatase; Bone Neoplasms; Carboxylesterase; Carboxylic Ester Hydrolases; Fibrous Dysplasia of Bone; Giant Cell Tumors; Humans; NADH Tetrazolium Reductase; Osteoclasts; Osteosarcoma; Sarcoma, Ewing; Sarcoma, Synovial

An atypical variant of Ewing's sarcoma, located in the left hip of a nine-year-old girl, is discussed at optical, histochemical and electron microscopical level. The endothelial appearing cells seem to play a main role in its histogenesis. Tumoral cells of an undifferentiated blastemic nature show round nuclei and bright lucent cytoplasm, being organized in solid sheets or vascular-like profiles. Alkaline and acid phosphatases are very prominent in all tumoral cells, and some of them also show PAS positive material. Its ultrastructure demonstrates an active pinocytic capacity, cytoplasmic filaments and Weibel-Palade bodies. Simultaneously a review is performed on 27 cases of typical Ewing's sarcoma of bone in order to compare its vessels of a reactive nature with this tumor. A differential diagnosis with hemangioendothelioma primary to bone is established.

Topics: Acid Phosphatase; Alkaline Phosphatase; Blood Vessels; Bone Neoplasms; Child; Endothelium; Female; Hip; Humans; Microscopy, Electron; Pinocytosis; Sarcoma, Ewing

In addition to a light-microscopical and histochemical investigation of primary and metastic lesions in 27 cases of Ewing's sarcoma, biopsy materials from the primary bone lesions of 7 patients with this neoplasm were examined histochemically, enzyme-cytochemically and electron microscopically to elucidate the histogenesis and nature of the neoplasm. Ultrastructural observation has revealed that besides intracytoplasmic and extracellular deposition of glycogen the tumor cells possess several cytological features characterized by intracytoplasmic microfilaments of varying thickness up to 80 A, occasional appearance of dense patches, fat droplets, desmosomal connections and reminiscent attachment bodies. The tumor cells are mostly round, oval or polygonal in shape, but spindle or elongated cells are intermingled and occasionally contain well-developed rough endoplasmic reticulum, resembling pericytes or fibroblasts. In the intercellular spaces amongst the tumor cells, varying amounts of variable-shaped amorphous materials are found, which are compatible with acid mucopolysaccharides and glycoproteins histochemically verified. These findings may suggest that Ewing's sarcoma is a highly malignant neoplasm originating from a transitional cell developed from pericytes to vascular smooth muscle cells in the bone marrow.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Bone Neoplasms; Glycogen; Humans; Sarcoma, Ewing

Topics: Acid Phosphatase; Adolescent; Adult; Bone Neoplasms; Child; Child, Preschool; Female; Fibrosarcoma; Fibrous Dysplasia of Bone; Giant Cell Tumors; Humans; Infant; Isoenzymes; Male; Middle Aged; Osteosarcoma; Sarcoma, Ewing; Soft Tissue Neoplasms

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Bone Neoplasms; Carcinoma; Esterases; Glucuronidase; Histocytochemistry; Hodgkin Disease; Humans; Leukemia, Lymphoid; Leukemia, Myeloid; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Monoamine Oxidase; Multiple Myeloma; Neoplasm Metastasis; Neuroblastoma; Plasmacytoma; Sarcoma, Ewing

Topics: Acid Phosphatase; Alkaline Phosphatase; Bone Neoplasms; Histocytochemistry; Humans; Lymphoma, Large B-Cell, Diffuse; Osteosarcoma; Sarcoma, Ewing

Topics: Acid Phosphatase; Alkaline Phosphatase; Bone Neoplasms; Chondroma; Chondrosarcoma; Diagnosis, Differential; Giant Cell Tumors; Hemangiosarcoma; Histocytochemistry; Humans; Lymphoma, Large B-Cell, Diffuse; Methods; Osteoma, Osteoid; Osteosarcoma; Sarcoma, Ewing; Sarcoma, Synovial

Topics: Acid Phosphatase; Alkaline Phosphatase; Bone Neoplasms; Carcinoma; Glycosaminoglycans; Humans; Neoplasm Metastasis; Sarcoma, Ewing; Staining and Labeling

Topics: Acid Phosphatase; Adenoma; Alkaline Phosphatase; Ameloblastoma; Arthritis; Bone Cysts; Bone Neoplasms; Chondrosarcoma; Fibroma; Fibrous Dysplasia of Bone; Geriatrics; Giant Cell Tumors; Humans; Neoplasm Metastasis; Osteitis Fibrosa Cystica; Osteoma; Osteosarcoma; Prognosis; Radiography; Sarcoma, Ewing; Tuberculosis; Tuberculosis, Osteoarticular