acid-phosphatase and Rotavirus-Infections

The effect of rotaviruses and enterotoxigenic Escherichia coli administered in various sequences to cesarean-derived, colostrum-deprived calves was studied using light and electron microscopy. The structure of the lymphoid tissue in the ileum, the number of mitoses in the crypts, number of intraepithelial lymphocytes, and enzyme histochemistry (alkaline phosphatase, acid phosphatase, succinic dehydrogenase, beta-galactosidase, and leucinaminopeptidase) of the ileal dome epithelium were evaluated. The area of lymphoid follicles in Peyer's patches of the ileum was investigated morphometrically. Monoinfections with either rotavirus or enterotoxigenic E. coli induced a significant increase in lymphoid follicle area, but did not affect dome epithelial cells. Dual infections did not consistently affect the follicle area, but the number of intraepithelial lymphocytes and the mitotic indices exceeded those of comparable monoinfections. Changes in activity of enzymes in the ileal dome epithelial area were minor.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; beta-Galactosidase; Cattle; Escherichia coli Infections; Histocytochemistry; Ileum; Leucyl Aminopeptidase; Microscopy, Electron; Mitotic Index; Peyer's Patches; Rotavirus Infections; Succinate Dehydrogenase

The effect of rotavirus and enterotoxigenic Escherichia coli, administered in different sequences, on alkaline and acid phosphatase, leucinaminopeptidase, beta-galactosidase, and succinicdehydrogenase of the intestinal mucosa of cesarian-derived, colostrum-deprived calves was investigated. Decrease in enzyme activity was most prominent in dual infections; it also occurred in parts of the small intestine in monoinfected animals. Increases in enzyme activity involved totally either one or all tissue compartments (crypt, basal villus area, villus tips). Increased activity was present in enteric mucosae that were either not affected or were only slightly affected by rotavirus or enterotoxigenic E. coli. We interpret the increase in enzyme activity as an adaptation of the enteric mucosa to maintain the absorptive function.

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; beta-Galactosidase; Cattle; Escherichia coli Infections; Histocytochemistry; Intestinal Mucosa; Intestine, Small; Kinetics; Leucyl Aminopeptidase; Microvilli; Rotavirus Infections; Succinate Dehydrogenase