acid-phosphatase and Retinitis-Pigmentosa

To compare the activities of acid phosphatase, N-acetyl-beta-glucosaminidase and alpha-mannosidase in cultured retinal pigment epithelium (RPE) and glial cells of Royal College of Surgeons (RCS) rat with those in Long Evans (LE).. The cultured RPE and glial cells of RCS and LE rat were plated into the same 96 well microtitre, and the biochemical method in microsystem were used for enzyme assays.. The activities of acid phosphatase and N-acetyl-beta-glucosaminidase are higher by, respectively, 30% and 46% in cultured RPE of RCS rat than LE rat. The activity of alpha-mannosidase has no significant difference. The activities of 3 enzymes in the retinal glial cells derived from RCS rats are higher than LE rat by 13% to 77%.. These results suggest that the high activities of lysosomal enzymes in RCS RPE and glial cells may play an important role in the pathogenesis of retinal dystrophy.

Topics: Acetylglucosaminidase; Acid Phosphatase; alpha-Mannosidase; Animals; Cells, Cultured; Mannosidases; Neuroglia; Pigment Epithelium of Eye; Rats; Retinitis Pigmentosa

The retinae of 14-day-old Hunter dystrophic rats have been subjected to low-energy irradiation by a pulsed ruby laser. Fifteen days after exposure, pigment epithelial cells had proliferated and repopulated the irradiated areas. In all such areas the subretinal photoreceptor debris had been reduced or lost.

Topics: Acid Phosphatase; Animals; Lasers; Lysosomes; Microscopy, Electron; Phagocytosis; Pigment Epithelium of Eye; Rats; Retinitis Pigmentosa

Topics: Abnormalities, Multiple; Acid Phosphatase; Arabinose; Corneal Opacity; Cytoplasmic Granules; Fibroblasts; Galactosidases; Glucosidases; Glucuronidase; Glycosaminoglycans; Glycoside Hydrolases; Hexosaminidases; Humans; Intellectual Disability; Mannose; Metabolism, Inborn Errors; Mucopolysaccharidoses; Phosphoric Diester Hydrolases; Retinitis Pigmentosa; Skin

Topics: Acid Phosphatase; Animals; Epithelial Cells; Epithelium; Extracellular Space; Histocytochemistry; Lysosomes; Microscopy, Electron; Phagocytes; Rats; Rats, Inbred Strains; Retina; Retinitis Pigmentosa

Topics: Acid Phosphatase; Carbohydrate Metabolism, Inborn Errors; Child; Child, Preschool; Glycosaminoglycans; Histocytochemistry; Humans; Inclusion Bodies; Infant; Intellectual Disability; Lymphocytes; Male; Microscopy, Electron; Mucopolysaccharidoses; Retinitis Pigmentosa; Staining and Labeling

Topics: Acid Phosphatase; Biopsy; Carbohydrate Metabolism, Inborn Errors; Child; Child, Preschool; Cytoplasm; Female; Fibroblasts; Glycosaminoglycans; Histocytochemistry; Humans; Infant; Intellectual Disability; Lysosomes; Macrophages; Male; Microscopy, Electron; Mucopolysaccharidoses; Retinitis Pigmentosa; Schwann Cells; Skin; Syndrome

Topics: Acid Phosphatase; Amidohydrolases; Animals; Female; Histocytochemistry; Lysosomes; Male; Naphthalenes; Peptide Hydrolases; Rats; Retina; Retinal Degeneration; Retinitis Pigmentosa

Topics: Acid Phosphatase; Age Factors; Animals; Cell Membrane Permeability; Centrifugation, Density Gradient; Leucyl Aminopeptidase; Lysosomes; Peptide Hydrolases; Rats; Rats, Inbred Strains; Retinal Pigments; Retinitis Pigmentosa; Serum Albumin, Bovine; Tyrosine

Topics: Acid Phosphatase; Animals; Lysosomes; Permeability; Rats; Retina; Retinitis Pigmentosa

Topics: Acid Phosphatase; Brain; Carbohydrate Metabolism, Inborn Errors; Chromatography, Gel; Fucose; Galactosidases; Glucosamine; Glucuronidase; Glycosaminoglycans; Glycoside Hydrolases; Hot Temperature; Humans; Intellectual Disability; Kidney; Mannose; Molecular Weight; Mucopolysaccharidoses; Mucopolysaccharidosis IV; Retinitis Pigmentosa; Spleen

Topics: Acid Phosphatase; Adolescent; Humans; Hyaluronoglucosaminidase; Prognosis; Retinitis Pigmentosa; Therapeutics; Vision Tests