acid-phosphatase and Retinal-Detachment

In rats with primary retinal degeneration, lens extraction combined with total retinal detachment provided a model for injection of a tracer of colloidal carbon into the subretinal space. Electron microscopy and acid phosphatase cytochemistry were subsequently used to analyze the ingestion of tracer by the retinal pigment epithelium. It was found that the attachment, ingestion, and digestion phases of the phagocytic process were apparently preserved. From this evidence it is suggested that there is no lack of phagocytic power in the retinal pigment epithelium of affected rat strains.

Topics: Acid Phosphatase; Animals; Carbon Radioisotopes; Cytoplasmic Granules; Phagocytosis; Pigment Epithelium of Eye; Rats; Retinal Degeneration; Retinal Detachment

Samples of subretinal fluid (SRF) from patients with primary rhegmatogeneous retinal detachment of various duration were studied using standard enzymologic techniques. The protein content in SRF increased with the duration of detachment. The activities of lysosomal enzymes in SRF, which were acid phosphatase, beta-glucuronidase, and cathepsin D, also increased with the duration of detachment. Especially cathepsin D, which was not detected in serum, was present in SRF. The activity of a nonlysosomal enzyme, i. e., lactic dehydrogenase, which was used as a marker of cell disruption and of serum transudation was the same as that of serum. Because lysosomal enzymes are known to be able to degrade cells and tissues, the enzymologic analysis of SRF contributes to our understanding of the pathology of primary rhegmatogeneous retinal detachment.

Topics: Acid Phosphatase; Cathepsins; Exudates and Transudates; Glucuronidase; Humans; Hydrolases; L-Lactate Dehydrogenase; Lysosomes; Retinal Detachment; Vitreous Body

Topics: Acid Phosphatase; Adult; Animals; Azathioprine; Blood Proteins; Cattle; Cyclophosphamide; Electrophoresis; Exudates and Transudates; Eye Diseases; Female; Humans; Isoenzymes; Lens, Crystalline; Potassium; Prednisone; Proteins; Retinal Detachment; Serum Albumin; Sodium; Uvea

Topics: Acid Phosphatase; Autopsy; Body Fluids; Cytoplasmic Granules; Electrophoresis, Polyacrylamide Gel; Epithelial Cells; Esterases; Histocytochemistry; Humans; Isoenzymes; L-Lactate Dehydrogenase; Macrophages; Retina; Retinal Detachment; Time Factors

Topics: Acid Phosphatase; Animals; Electrophoresis, Polyacrylamide Gel; Eye Diseases; Histocytochemistry; Isoenzymes; Leukocytes; Lymphocytes; Membranes; Ophthalmoscopy; Rabbits; Retinal Detachment; Vitreous Body