acid-phosphatase and Pyelonephritis

Pyelonephritis is the most common urinary tract infection affecting females of all age groups. Despite concerted efforts the mechanism of renal injury in pyelonephritis is not clearly understood. In the present study we have made an attempt to characterise the mediators of inflammatory insult in an experimental model of ascending pyelonephritis. Mice infected with Escherichia coli O6:K13:H1 were sacrificed at 2, 7 and 14 days post-infection. Luminol-dependent chemiluminescence response, NADPH oxidase, acid phosphatase, beta-glucuronidase and N-acetyl-beta-D-glucosaminidase activities were monitored in circulating as well as renal phagocytic cells in order to determine the role of reactive oxygen species and lysosomal enzymes in genesis of renal injury. We have demonstrated that reactive oxygen species are generated at the initiation of infection and the levels increase progressively during the course of infection. While intracellular release of lysosomal enzymes was seen in all groups, extracellular release was primarily observed at 7 and 14 days post-infection only. The results indicate that while reactive oxygen species play a significant role in tissue injury during all stages of infection, lysosomal enzyme release in extracellular milieu augments tissue destruction at later stages only.

Topics: Acetylglucosaminidase; Acid Phosphatase; Animals; Disease Models, Animal; Female; Glucuronidase; Kidney; Luminescent Measurements; Lysosomes; Mice; NADPH Oxidases; Pyelonephritis; Reactive Oxygen Species

Topics: Acid Phosphatase; Adolescent; Alkaline Phosphatase; Cell Membrane; Child; Child, Preschool; Diphosphonates; Glomerulonephritis; Glucocorticoids; Humans; Hydrocortisone; Leukocytes; Peroxidase; Polyvinyl Alcohol; Prednisone; Pyelonephritis; Urine

Experimental retrograde E. coli pyelonephritis was produced in rats. The study covered the period from 6-24 hours up to 6 months. Macrophages in the renal tissue were studied using immunofluorescence staining for bacterial E. coli antigen and histochemical staining for aicd phosphatase. A comparison of sections stained according to the two methods showed that antigen-containing macrophages in nearly all cases yielded a positive reaction for acid phosphatase. On the other hand, in several kidneys acid phosphatase-positive macrophages occurred which in consecutive sections studied by immunofluorescence did not contain antigen. The possibility of using staining for acid phosphatase as a screening method for the detection of active, antigen-containing macrophages in human chronic pyelonephritis is discussed.

Topics: Acid Phosphatase; Animals; Antigens, Bacterial; Escherichia coli; Female; Macrophages; Pyelonephritis; Rats; Staining and Labeling; Time Factors

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Disease Models, Animal; Fibroblasts; Kidney Cortex; Kidney Medulla; Kidney Tubules; Macrophages; Microscopy, Electron; Pyelonephritis; Rabbits; Staining and Labeling; Time Factors

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Esterases; Female; Humans; Kidney; Male; Microtomy; Middle Aged; Pyelonephritis; Succinate Dehydrogenase

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Cholinesterases; Enzymes; Ischemia; Kidney; Kidney Diseases; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Malate Dehydrogenase; Male; Pyelonephritis; Rabbits; Transaminases

Topics: Acid Phosphatase; Animals; Chronic Disease; Disease Models, Animal; Kidney Glomerulus; Kidney Medulla; Kidney Tubules; Male; Pyelonephritis; Rabbits

Topics: Acid Phosphatase; Alanine Transaminase; Alkaline Phosphatase; Animals; Aspartate Aminotransferases; Cholinesterases; Diagnosis, Differential; Enzymes; Hypoxia; Kidney Diseases; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Malate Dehydrogenase; Male; Mercury Poisoning; Pyelonephritis; Rabbits

Topics: Acid Phosphatase; Alkaline Phosphatase; Balneology; Bulgaria; Chronic Disease; Health Resorts; Humans; Kidney Calculi; Pyelonephritis; Urologic Diseases

Topics: Acid Phosphatase; Acidosis, Renal Tubular; Adult; Female; Humans; Hypokalemia; Nephrocalcinosis; Pyelonephritis

Topics: Acid Phosphatase; Alanine Transaminase; Alkaline Phosphatase; Animals; Aspartate Aminotransferases; Biochemical Phenomena; Biochemistry; Colorimetry; Enzymes; Glutamate Dehydrogenase; Humans; In Vitro Techniques; Malate Dehydrogenase; Models, Theoretical; Phospholipids; Pyelonephritis; Rabbits

Topics: Acid Phosphatase; Clinical Enzyme Tests; Cystitis; Glomerulonephritis; Humans; Kidney Diseases; Pyelonephritis; Uremia