acid-phosphatase and Psoriasis

Topics: Acid Phosphatase; Aged; Arthritis, Rheumatoid; Body Temperature; Calcinosis; Female; Humans; Inflammation; Joint Diseases; Kinins; Knee Joint; Lysosomes; Male; Middle Aged; Proteins; Psoriasis; Synovial Fluid

Topics: Acid Phosphatase; Adult; Epidermis; Histocytochemistry; Humans; Microscopy, Electron; Middle Aged; Psoriasis

Topics: Acid Phosphatase; Adult; Arylsulfatases; Deoxyribonucleases; Female; Humans; Lysosomes; Male; Middle Aged; Psoriasis; Ribonucleases; Skin

Topics: Acid Phosphatase; Arthritis; Genetic Markers; Haptoglobins; HLA Antigens; HLA-A Antigens; HLA-B Antigens; Humans; Immunoglobulin Allotypes; Immunoglobulin G; Isoenzymes; Phosphoglucomutase; Psoriasis

Topics: Acid Phosphatase; Adolescent; Adult; Child; Female; Humans; Leukocyte Count; Male; Middle Aged; Neutrophils; Psoriasis

Topics: Acid Phosphatase; Adolescent; Adult; Child; Female; Humans; Lymphocytes; Male; Middle Aged; Neutrophils; Psoriasis

Three marker enzymes were measured during treatment of psoriatic plaques with two different therapies. During treatment with clobetasol propionate the epidermal enzymes (acid phosphatase and glucose-6-phosphate dehydrogenase) returned to normal within 14 days whereas capillary alkaline phosphatase remained at the original level. By contrast, all three marker enzymes reverted to normal at the same tempo during PUVA therapy, reaching the control range after 4-8 weeks.

Topics: Acid Phosphatase; Alkaline Phosphatase; Betamethasone; Clobetasol; Epidermis; Glucosephosphate Dehydrogenase; Humans; Photochemotherapy; Psoriasis; PUVA Therapy; Time Factors

Topics: Acid Phosphatase; Adolescent; Adult; Child; Female; Humans; Lymphocytes; Male; Middle Aged; Psoriasis

Test sites on healthy controls and on the clinically uninvolved skin of psoriatic patients were stripped with tape, and eight variables were quantified at intervals during the subsequent healing process. In the control groups, the stratum corneum regenerated at a constant rate and the underlying skin showed elevations of metabolic activity peaking around days 2-4. In the psoriatic groups, we observed that (I) the response of the keratinizing zone is identical to that of the controls, (2) the proliferative response is initially normal but remains elevated rather longer than usual, and (3) the dermal capillaries (indicated by alkaline phosphatase activity) show a gross hyper-reactivity which is already apparent after 1 day and which persists for more than a week. These findings support our previous conclusion that metabolic alteration of the dermal capillary precedes epidermal hyperplasia in the pathogenesis of the psoriatic lesion.

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Capillaries; Cell Division; Female; Glucosephosphate Dehydrogenase; Humans; Male; Psoriasis; Skin; Wound Healing

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Alkaline Phosphatase; Female; Humans; Hyaluronoglucosaminidase; Hydrolases; Male; Middle Aged; Psoriasis; Skin

Keratotome slices were cut across the margins of rapidly-spreading psoriatic plaques. Each slice was divided into eight sections and in each section we measured the percentage cells in S phase and the levels of glucose-6-phosphate dehydrogenase (both related to epidermal proliferation), acid phosphatase (associated with keratinization) and alkaline phosphatase (a marker for dermal capillaries). Disturbances in the epidermis extended only 2 to 4 mm into the 'uninvolved' skin, whereas the capillaries were metabolically abnormal for a distance of about 2 cm ahead of the advancing edge of the plaque. This implies that changes in the capillary may precede those in the epidermis during the spread of the psoriatic lesion.

Topics: Acid Phosphatase; Alkaline Phosphatase; Cell Cycle; DNA; Glucosephosphate Dehydrogenase; Humans; Kinetics; Psoriasis; Skin

Topics: Acid Phosphatase; Adolescent; Adult; Deoxyribonucleases; Female; Humans; Lysosomes; Male; Middle Aged; Photochemotherapy; Psoriasis; PUVA Therapy; Ribonucleases

Topics: Acid Phosphatase; Cyclic AMP; Deoxyribonucleases; Humans; Lysosomes; Psoriasis; Ribonucleases; Skin

The 5'-nucleotidase activity in psoriatic and normal human epidermis was studied in comparison to acid phosphatase activity. The optimum pH in normal human epidermis was about 5.0 at room temperature. The activity of both enzymes was found to be high in the transitional zone. Acid phosphatase (non-specific) activity was strongly positive in the psoriatic parakeratotic horny layers whereas 5'-nucleotidase activity in that area was completely absent. The results suggest that the enzyme which degrades nucleoside-5'-phosphate to nucleoside and inorganic phosphate is not acid phosphatase but 5'-nucleotidase. Nuclear preservation in psoriatic hyperkeratosis was attributed to absence or inactivation of specific enzymes of nuclear degradation, such as 5'-nucleotidase, rather than acid phosphatase.

Topics: Acid Phosphatase; Adult; Epidermis; Humans; Hydrogen-Ion Concentration; Keratosis; Nucleotidases; Parakeratosis; Psoriasis; Skin

Isoelectric focusing and concanavalin A-Sepharose chromatography were used to study the multiple forms and glycoprotein natures of so-called lysosomal hydrolases from psoriatic scales. Acid phosphatase appeared as 5 different forms with pI values of 6.5, 6.1, 5.8, 5.6 and 5.45. Seven isoenzymes of alpha-fucosidase were identified with pI values of 6.4, 6.2, 5.9, 5.75, 5.65, 5.4 and 5.2. Acid alpha-mannosidase activity appeared as one peak with pI value of 6.75 and a weak activity of neutral alpha-mannosidase was present with pI value of 6.7. Incubation of the extract with neuraminidase increased their pI values of acid phosphatase, alpha-fucosidase and alpha-mannosidase to the more basic forms. This finding suggests that epidermal acid phosphatase, alpha-fucosidase and alpha-mannosidase have some N-acetylneuraminic acid residues. In addition concanavalin A-Sepharose column chromatography was also performed to confirm the glycoprotein nature of acid phosphatase. This enzyme was bound to the column and not released from the column even with the treatment of 0.5 M NaCl, but the enzyme was eluted from the column with the treatment of alpha-methyl-D-glucoside.

Topics: Acid Phosphatase; alpha-L-Fucosidase; Humans; In Vitro Techniques; Isoelectric Focusing; Mannosidases; Psoriasis; Skin

Damaged epidermal cells present in psoriatic lesions were investigated using histochemical and histomorphological methods including ultrathick sections. In early lesions these cells were found throughout the epidermis, being particularly numerous in the upper part of the latter and around the adnexa. In a later developmental stage the damage to the keratinocytes in the suprapapillary region was found to precede the damage to the basement membrane. These findings, together with the difference between the damaged keratinocytes lining the ascending side and the venous limb of the papilla respectively with regard to degree of severity of the damage, suggest that blood stasis is the cause of the regressive changes in the suprapapillary epidermis. The enzymatic activity of non-specific esterases and acid phosphatases was increased and that of succindehydrogenases and DPN-diaphorase was decreased in damage keratinocytes. The significance of these findings in relation to the genesis and development of psoriasis is discussed. The view is expressed that there is a causal relationship between damage to the epidermal cells and proliferation of epidermis.

Topics: Acid Phosphatase; Basement Membrane; Cell Count; Epidermis; Esterases; Histocytochemistry; Humans; NADPH Dehydrogenase; Psoriasis; Succinate Dehydrogenase

Polymorphism of red cell acid phosphatase (ACP-1; E.C. 3.1.3.2.) has been studied using the Cellogel technique of Martin et al. (1975) in 102 patients with psoriasis and 102 healthy controls. In contrast to two previous reports, no anomalies in distribution of phenotypes were found in patients or in controls.

Topics: Acid Phosphatase; Erythrocytes; Female; Humans; Male; Phenotype; Polymorphism, Genetic; Psoriasis

Topics: Acid Phosphatase; Adult; Anthracenes; Anthralin; Female; Glucosephosphate Dehydrogenase; Humans; L-Lactate Dehydrogenase; Male; Psoriasis

Topics: Acid Phosphatase; Animals; Cathepsins; Chediak-Higashi Syndrome; Cyclic AMP; Dermatitis; Fabry Disease; Humans; Keratins; Lupus Erythematosus, Systemic; Lysosomes; Psoriasis; Skin; Ultraviolet Rays; Vacuoles; Vitamin A Deficiency

In a sample of n = 160 nonrelated male and female patients suffering from psoriasis Vulgaris, blood serum protein, and enzyme group typings have been carried out and compared with healthy controls from the same area (Rheinland-Pfalz). Marked statistically significant differences between patients and controls were found in none of the genetic blood polymorphisms considered here. However, combining previously published data from various authors with our own, significant associations between this skin disease and genetic polymorphisms such as MN, Gc, Gm (2), red cell acid phosphatase, and red cell phosphoglucomutase (PGM1) were seen. The possible reasons for these associations are discussed.

Topics: Acid Phosphatase; Blood Group Antigens; Blood Proteins; Erythrocytes; Female; Humans; Male; MNSs Blood-Group System; Phosphoglucomutase; Polymorphism, Genetic; Psoriasis

Seven red cell enzyme systems were examined for electrophoretic variation in a series of psoriatic patients. In two polymorphic (ADA, ES D) and four non-polymorphic systems (SOD, MDH, LDH, Dia) no deviations from normal controls were found. In the acid phosphatase system the frequency of the pa gene was found to be decreased.

Topics: Acid Phosphatase; Adenosine Deaminase; Dihydrolipoamide Dehydrogenase; Erythrocytes; Esterases; Gene Frequency; Genetic Variation; Humans; L-Lactate Dehydrogenase; Malate Dehydrogenase; Psoriasis; Superoxide Dismutase

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; DNA; Eczema; Female; Glycosaminoglycans; Humans; Male; Middle Aged; Mud Therapy; Neurodermatitis; Psoriasis; RNA; Skin

Topics: Acid Phosphatase; Adenosine Triphosphatases; Enzyme Activation; Enzyme Repression; Humans; Nucleotidases; Phosphoric Monoester Hydrolases; Phosphorylases; Psoriasis; Skin

Electronmicroscopical investigation on 12 cutaneous biopsies of different localisation showed that the seborrhoic dermatitis is not comparable to a psoriatic tissue-reaction. Both, the cyto-morphological feature and the demonstrated localisation of the acid phosphatase are distinctly different from that of the psoriatic epidermis. The described changes are clearly more similar to the well known ultrastructural pictures of eczemateous reactions. In spite of the "eczema-like" ultrastructural picture seborrhoic dermatitis apparently can be separated from the allergic and irritant contact-dermatitis. Nevertheless, the epidermal alterations are also similar to chronic nummular eczema. They therefore are unspecific and do not allow any conclusion regarding the etiopathogenesis or nosological classification of this skin disease.

Topics: Acid Phosphatase; Basement Membrane; Cell Nucleus; Dermatitis, Contact; Dermatitis, Seborrheic; Desmosomes; Eczema; Endoplasmic Reticulum; Histocytochemistry; Humans; Keratins; Organoids; Phagocytes; Psoriasis; Skin; Vacuoles

Disc-electrophoretic investigations of psoriatic scale homogenates (15000 x g supernatant) revealed several different phosphatase activities. At pH 5 either five different phosphatase active bands (substrates: p-nitrophenylphosphate, naphthylphosphate) or three different bands (substrate: glycerophosphate) could be obtained. At pH 7 only one band (substrate: p-nitrophenylphosphate) showed phosphatase activity. At pH 9.9 either two bands (substrate: p-nitrophenylphosphate) or three bands (substrate: glycerophosphate) could be demonstrated. the acid p-nitrophenylphosphatase activity of the psoriatic specific bands "9" and "10" could be distinguished by their different fluoride and tartrate susceptibility. Also the alkaline glycerophosphatase activities could be differentiated by distinct Ca and Mg susceptibility.

Topics: Acid Phosphatase; Alkaline Phosphatase; Calcium; Cysteine; Electrophoresis, Disc; Fluorides; Glycerophosphates; Humans; Hydrogen-Ion Concentration; Magnesium; Molecular Weight; Naphthols; Nitrophenols; Phosphoric Monoester Hydrolases; Psoriasis; Tartrates

The effects of methotrexate and azathioprine, two drugs used in antipsoriatic therapy, on oxygen consumption of surviving human skin and on enzymatic activities of human skin homogenates were investigated. In concentrations of 1 mM/1, both substances provoked a significant decrease in oxygen consumption of human skin; in this respect, there was practically no difference between methotrexate and azathioprine. In the enzyme assays, however, azathioprine was, by far, less effective than methotrexate. After an incubation of 120 min azathioprine (1mM/1) inhibited lactate and glucose-6-phosphate dehydrogenase activities by about 10 per cent only, whereas the corresponding values with methotrexate amounted to 80 and 70 per cent, respectively. Methotrexate revealed an immediate inhibitory effect on pure glucose-6-phosphate dehydrogenase whereas azathioprine produced no changes in this mode. Furthermore, only methotrexate inhibited "acid" phosphatase activity of human skin homogenates.--These data sustain the theory that the better clinical efficacy of methotrexate in patients with psoriasis might be due to the more pronounced inhibition of important enzymes such as the enzymes of the pentose phosphate shunt.

Topics: Acid Phosphatase; Azathioprine; Glucosephosphate Dehydrogenase; Humans; In Vitro Techniques; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Methotrexate; Oxygen Consumption; Pentosephosphates; Psoriasis; Skin

Twelve phenolic fractions of creosote and anthracene oils derived from a high temperature tar were applied in an ointment base to mouse tail skin. After treatment with the higher boiling acids, formerly parakeratotic scale areas underwent granular layer induction and 'basket-weave' keratin was produced. Changes in distribution of acid phosphatase and in horny layer fluorescence were consistent with the conversion to an orthokeratotic state. It is suggested that some of these phenols may be of value in the treatment of chronic psoriasis.

Topics: Acid Phosphatase; Animals; Anthracenes; Coal Tar; Creosote; Keratins; Mice; Parakeratosis; Phenols; Psoriasis; Skin

Topics: Acid Phosphatase; Aspartate Aminotransferases; Electrophoresis, Starch Gel; Erythrocytes; Genetic Variation; Germany, West; Glucosephosphate Dehydrogenase; Humans; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; NADP; Phenotype; Phosphoglucomutase; Polymorphism, Genetic; Psoriasis

Topics: Acid Phosphatase; Centrifugation; Chromatography, Affinity; Electrophoresis, Disc; Histocytochemistry; Humans; Lysosomes; Psoriasis; Ribosomes; Skin

Topics: Acid Phosphatase; Beryllium; Chloromercuribenzoates; Copper; Cytoplasm; Fluorides; Humans; Lead; Psoriasis; Skin; Tartrates; Zinc

Topics: Acid Phosphatase; Biopsy; Glucosephosphate Dehydrogenase; Glyceraldehyde-3-Phosphate Dehydrogenases; Glycerolphosphate Dehydrogenase; Histocytochemistry; Humans; NAD; NADP; Psoriasis; Skin; Staining and Labeling

Topics: Acid Phosphatase; Biopsy; Cicatrix; Esterases; Histocytochemistry; Humans; Lipids; Psoriasis; Skin

Topics: Acid Phosphatase; Dermatitis, Atopic; Esterases; Fructose-Bisphosphate Aldolase; Glucosephosphate Dehydrogenase; Glucuronidase; Glutamate Dehydrogenase; Glyceraldehyde-3-Phosphate Dehydrogenases; Glycerolphosphate Dehydrogenase; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Malate Dehydrogenase; Phosphogluconate Dehydrogenase; Psoriasis

Topics: Acid Phosphatase; Esterases; Glucosephosphate Dehydrogenase; Histocytochemistry; Histological Techniques; Humans; L-Lactate Dehydrogenase; Oxidoreductases; Phosphogluconate Dehydrogenase; Phospholipids; Psoriasis; Regeneration; Skin; Succinate Dehydrogenase

Topics: Acid Phosphatase; Animals; Citric Acid Cycle; Foot; Glucosephosphate Dehydrogenase; Guinea Pigs; Hair; Hand; Histocytochemistry; Humans; Mice; Mitochondria; Pentosephosphates; Psoriasis; Skin; Tail

Topics: Acid Phosphatase; Alkaline Phosphatase; Electron Transport; Electron Transport Complex IV; Esterases; Exudates and Transudates; Glucosephosphate Dehydrogenase; Glyceraldehyde-3-Phosphate Dehydrogenases; Glycerolphosphate Dehydrogenase; Histocytochemistry; Humans; Isocitrate Dehydrogenase; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Malate Dehydrogenase; NAD; NADP; Oxidoreductases; Peroxidases; Plastics; Psoriasis; Succinate Dehydrogenase; Tetrazolium Salts; Ubiquinone; Water

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Biopsy; Female; Griseofulvin; Histocytochemistry; Humans; Male; Middle Aged; Psoriasis; Skin; Succinate Dehydrogenase

Topics: Acid Phosphatase; Esterases; Histocytochemistry; Humans; Psoriasis; Skin

Topics: Acid Phosphatase; Extracellular Space; Histocytochemistry; Humans; Lysosomes; Microscopy, Electron; Psoriasis; Skin

Topics: Acid Phosphatase; Esterases; Formaldehyde; Freeze Drying; Freezing; Histocytochemistry; Humans; Paraffin; Psoriasis; Skin

Topics: Acid Phosphatase; Histocytochemistry; Humans; Keratins; Microscopy, Electron; Psoriasis; Skin

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Anthracenes; Anti-Infective Agents, Local; Esterases; Female; Histocytochemistry; Humans; Lipids; Middle Aged; Nucleic Acids; Phospholipids; Psoriasis; Skin

Topics: Acid Phosphatase; Biopsy; Cytoplasm; Electrons; Epidermis; Histocytochemistry; Humans; Lysosomes; Microscopy; Microscopy, Electron; Psoriasis; Skin

Topics: Acid Phosphatase; Adolescent; Clinical Enzyme Tests; Geriatrics; Humans; Psoriasis

Topics: Acid Phosphatase; Alkaline Phosphatase; Capillaries; Connective Tissue; Humans; Phosphoric Monoester Hydrolases; Psoriasis; Sweat Glands

Topics: Acid Phosphatase; Humans; Phosphoric Monoester Hydrolases; Psoriasis