acid-phosphatase and Polycythemia-Vera

Topics: Acid Phosphatase; Aged; Female; Humans; Male; Middle Aged; Polycythemia Vera

The occurrence of natural substances with antigenic properties similar to those of the prostatic acid phosphatase was examined in one patient with neutrophilic leukemia and increased activity of serum acid phosphatase. The fraction responsible for the increased serum enzyme activity was tartrate sensitive and was identified as isoenzyme 2 by polyacrylamide gel electrophoresis. This isoenzyme originated from the leukocytes but had similar electromobility to that of the prostatic acid phosphatase isoenzyme 2. Immunohistochemical and counterimmunoelectrophoretic studies indicated that this leukocytic isoenzyme was present in the neutrophils and shared antigenic properties with the prostatic isoenzyme 2. Leukocytes from one patient with acute granulocytic leukemia, two patients with polycythemia vera with neutrophilia, and five normal subjects also contained this prostatic acid phosphatase like isoenzyme. Elevated serum "prostatic" acid phosphatase activity, therefore, may be found not only in prostatic cancer but also in granulocytic leukemia and perhaps other diseases.

Topics: Acid Phosphatase; Adult; Aged; Agranulocytosis; Counterimmunoelectrophoresis; Electrophoresis, Polyacrylamide Gel; Histocytochemistry; Humans; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Male; Middle Aged; Neutrophils; Polycythemia Vera; Prostate; Prostatic Neoplasms

Bone marrow smears of 48 patients consisting of 12 normal cases, 36 patients with different haematological diseases-among them 9 cases of idiopathic thrombopenia, 4 cases of polycythaemia, and 9 cases of Hodgkin's disease - were examined cytochemically. Acid phosphatase, unspecific esterases, naphthol-AS-D-chloroacetate esterase, peroxydase, and leucin-aminopeptidase were represented; in addition the PAS reaction, fastgreen staining at pH 1.1, methyl-green pyronin staining and the lipid representation with Sudan black B were carried out. Besides those responses known from literature the different behaviour of acid megacaryocyte phosphatase in different haematological diseases must be particularly emphasized from all reactions.

Topics: Acid Phosphatase; Alkaline Phosphatase; Aminopeptidases; Blood Proteins; Esterases; Hematologic Diseases; Hodgkin Disease; Humans; Megakaryocytes; Peroxidases; Polycythemia Vera; Polysaccharides; Thrombocytopenia

Topics: Acid Phosphatase; Blood Platelets; Bone Marrow; Bone Marrow Cells; Chromatography, DEAE-Cellulose; Chromatography, Ion Exchange; Electrophoresis, Disc; Fluorides; Gaucher Disease; Histocytochemistry; Humans; Isoenzymes; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Lymphatic Diseases; Lymphocytes; Lymphoma, Non-Hodgkin; Molecular Weight; Monocytes; Nitrophenols; Polycythemia Vera; Staining and Labeling; Tartrates

Topics: Acid Phosphatase; Diagnosis, Differential; Erythrocytes; Histocytochemistry; Humans; Leukemia, Erythroblastic, Acute; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Polycythemia Vera; Staining and Labeling

Topics: Acid Phosphatase; Bone Marrow Cells; Child; Clinical Enzyme Tests; Diagnosis, Differential; Erythropoiesis; Esterases; Histocytochemistry; Humans; Leukemia; Leukemia, Erythroblastic, Acute; Leukocyte Count; Pathology; Polycythemia Vera

Topics: Acid Phosphatase; Bone Marrow; Bone Marrow Cells; Esterases; Histocytochemistry; Humans; In Vitro Techniques; Leukemia, Erythroblastic, Acute; Polycythemia Vera

Topics: Acid Phosphatase; Anemia; Anemia, Aplastic; Azo Compounds; Blood Cells; Bone Marrow Cells; Histocytochemistry; Hodgkin Disease; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Multiple Myeloma; Mycosis Fungoides; Neoplasms; Polycythemia Vera; Sarcoma