acid-phosphatase and Pituitary-Neoplasms

We have compared the properties of pp60c-src from the mouse pituitary tumor cell line, AtT20, and from mouse fibroblasts. In vitro, pp60c-src phosphotransferase activity from AtT20 cells is 2- to 3-fold that of mouse NIH 3T3 fibroblast pp60c-src. In analyzing the reason for this elevation in specific activity, we found that pp60c-src from AtT20 cells differs structurally in at least three ways from pp60c-src in fibroblasts. First, AtT20 cells and primary rat anterior pituitary cells express low levels of the neuronal form of pp60c-src. Second, pp60c-src from AtT20 cells is phosphorylated at two additional N-terminal serine residues. Last, AtT20 pp60c-src is phosphorylated to a lower overall stoichiometry.

Topics: Acid Phosphatase; Animals; Electrophoresis, Polyacrylamide Gel; Fibroblasts; Immunosorbent Techniques; Isoelectric Point; Mice; Myristic Acid; Myristic Acids; Neurons; Phosphorylation; Phosphotransferases; Pituitary Gland; Pituitary Neoplasms; Protein-Tyrosine Kinases; Proto-Oncogene Proteins; Proto-Oncogene Proteins pp60(c-src); Tumor Cells, Cultured

The radioimmunoassay technique, first developed for the determination of hormones, has been applied to many substances of biologic interest by clinical and research laboratories around the world. It has had an enormous effect in medicine and biology as a diagnostic tool, a guide to therapy, and a probe for the fine structure of biologic systems. For instance, the assays of insulin, gastrin, secretin, prolactin, and certain tissue-specific enzymes have been invaluable in patient care. Further refinements of current methods, as well as the emergence of new immunoassay techniques, are expected to enhance precision, specificity, reliability, and convenience of the radioimmunoassay in both clinical and research laboratories.

Topics: Acid Phosphatase; Amylases; Animals; Cimetidine; Creatine Kinase; Dogs; Female; Gastrins; Hexosaminidases; Hormones, Ectopic; Humans; Hyperthyroidism; Insulin; L-Lactate Dehydrogenase; Middle Aged; Pancreatic Elastase; Pepsinogens; Peptic Ulcer; Peptides; Pituitary Neoplasms; Prolactin; Radioimmunoassay; Secretin; Species Specificity; Swine; Thyrotropin; Thyrotropin-Releasing Hormone

Combined immunohistochemical staining (IHCS) and enzyme histochemical staining (EHCS) methods for light microscopy (LM) and electron microscopy (EM) are reported, using oestrogen-induced rat pituitary tumours. For LM, combined staining for alkaline phosphatase and acid phosphatase by EHCS, using the azo dye method, and for prolactin and ACTH by IHCS, using the enzyme-labelled antibody method, gave the best results on 1 microgram glycol methacrylate sections. For EM, combined staining by EHCS on 30 microgram tissue sections followed by IHCS for prolactin on ultrathin Epon sections (enzyme-labelled antibody method) provided acceptable results. By these combined staining methods, the neoplastic prolactin cells were shown to have close affinity to rich alkaline phosphatase-positive capillaries and to possess an alkaline phosphatase-positive cell membrane. Furthermore, they revealed acid phosphatase-positive lysosomal and secretory granules. These combined staining methods may be valuable in studies on the actual functional status of cells.

Topics: Acid Phosphatase; Adenoma; Adrenocorticotropic Hormone; Alkaline Phosphatase; Animals; Female; Histocytochemistry; Immunoenzyme Techniques; Microscopy, Electron; Neoplasms, Experimental; Pituitary Neoplasms; Prolactin; Rats

Topics: Acid Phosphatase; Adrenal Cortex Hormones; Animals; Body Weight; Cell Line; Endoplasmic Reticulum; Epithelial Cells; Epithelium; Estrogens; Golgi Apparatus; Growth Hormone; Hormones, Ectopic; Inclusion Bodies; Leydig Cells; Male; Microscopy, Electron; Neoplasm Transplantation; Neoplasms, Experimental; Organ Size; Pituitary Neoplasms; Prolactin; Prostate; Rats; Rats, Inbred WF; Testis; Testosterone

Topics: Acid Phosphatase; Female; Galactorrhea; Golgi Apparatus; Histocytochemistry; Humans; Lysosomes; Pituitary Gland; Pituitary Gland, Anterior; Pituitary Neoplasms; Pregnancy; Prolactin; Secretory Rate

Topics: Acid Phosphatase; Adenoma; Endoplasmic Reticulum; Golgi Apparatus; Histocytochemistry; Humans; Lysosomes; Microscopy, Electron; Pituitary Neoplasms

Topics: Acid Phosphatase; Animals; Castration; Female; Galactosidases; Glucuronidase; Histocytochemistry; Hydrolases; Immunodiffusion; Lysosomes; Mammary Neoplasms, Experimental; Ovary; Pituitary Neoplasms; Rats; Rats, Inbred WF; Sulfatases

Topics: Acid Phosphatase; Adenoma, Chromophobe; Astrocytoma; Biopsy; Brain Neoplasms; Choroid Plexus; Ependymoma; Glioblastoma; Hemangiosarcoma; Histocytochemistry; Humans; Medulloblastoma; Meningioma; Neurilemmoma; Oligodendroglioma; Papilloma; Pituitary Neoplasms; Rosaniline Dyes; Sarcoma; Staining and Labeling

Topics: Acid Phosphatase; Adenoma, Chromophobe; Ameloblastoma; Astrocytoma; Biopsy; Brain; Brain Neoplasms; Cell Differentiation; Cerebellar Neoplasms; Cerebellum; Choroid Neoplasms; Choroid Plexus; Ependyma; Ependymoma; Esterases; Ganglia; Ganglioneuroma; Hemangiosarcoma; Histocytochemistry; Humans; Medulla Oblongata; Medulloblastoma; Meninges; Meningioma; Neuroectodermal Tumors, Primitive, Peripheral; Neuroma; Neurons; Oligodendroglioma; Pituitary Gland; Pituitary Neoplasms; Sarcoma

Topics: Acid Phosphatase; Adenoma, Chromophobe; Adenosine Triphosphatases; Alkaline Phosphatase; Brain Neoplasms; Glioblastoma; Histocytochemistry; Humans; Meningioma; Neurilemmoma; Peripheral Nervous System Neoplasms; Pituitary Neoplasms; Succinate Dehydrogenase; Vestibulocochlear Nerve

Topics: Acid Phosphatase; Adenoma; Brain Abscess; Brain Neoplasms; Cerebral Cortex; Ependymoma; Glioma; Histocytochemistry; Humans; Lymphoma, Large B-Cell, Diffuse; Medulloblastoma; Meningioma; Microscopy, Fluorescence; Pituitary Neoplasms; Staining and Labeling