acid-phosphatase and Peritoneal-Neoplasms

Cellular components in peritoneal fluid of infertile patients with and without endometriosis were evaluated in 102 patients with Wright's-Giemsa and Papanicolaou stains. The secretory activity of these cells was studied indirectly by assaying acid phosphatase, prostaglandin (PG) F2 alpha and PGE2 and complement components C3c and C4. The results showed that macrophages and lymphocytes were the dominant cells in peritoneal fluid of these patients. These cells were significantly increased in endometriosis patients, as compared with control subjects. In addition, peritoneal fluid acid phosphatase, PGF2 alpha and PGE2, and complement components C3c and C4 were significantly increased in patients with endometriosis. These cellular changes and their activation in peritoneal fluid may explain infertility associated with endometriosis.

Topics: Acid Phosphatase; Adolescent; Adult; Ascitic Fluid; Complement C3; Complement C3c; Complement C4; Dinoprost; Dinoprostone; Endometriosis; Fallopian Tube Patency Tests; Female; Humans; Infertility, Female; Lymphocytes; Macrophages; Peritoneal Neoplasms; Prostaglandins E; Prostaglandins F; Staining and Labeling

A carcinoma invasion system (Krebs-2 and Ehrlich tetraploid ascites tumors invading mouse peritoneum) was studied by high-voltage electron microscope (HVEM) stereoscopy, conventional (medium voltage) electron microscopy (MVEM), and cytochemistry. Tumor cells entered areas of peritoneum (mainly parietal) only where mesothelial cells were damaged and where there was inflammation of the underlying stroma. The initial invasion was different from that of most other invading carcinomas in that there was minimal breakdown of basal lamina and collagen. Neither tumor cells, inflammatory leukocytes nor peritoneal fibroblasts showed significant secondary lysosome production or release of intracellular or extracellular acid phosphatase. Morphological and cytochemical criteria suggest that in some invading carcinomas, as with non-tumor migrating cells such as leukocytes, widespread proteolysis due to diffusion of proteases is not a prerequisite for invasion of stromal connective tissue.

Topics: Acid Phosphatase; Animals; Ascites; Basement Membrane; Carcinoma; Collagen; Extracellular Matrix; Inflammation; Lysosomes; Mice; Peptide Hydrolases; Peritoneal Neoplasms

The histoenzymological and ultrastructural study of a haemangipericytoma with the clinical and histological characteristics of malignancy led to the identification within the tumour, in addition to pericytes and more differentiated cells, fibroblastic or muscular, of younger cells. The latter, arranged in cords undergo multiple appearances of transformation to result in a double maturation, at one and the same time pericytic and angio-endothelial, rarely seen up to the present in haemangipericytomas.

Topics: Acid Phosphatase; Adult; Alkaline Phosphatase; Hemangiopericytoma; Humans; Male; Oxidoreductases; Pelvic Neoplasms; Peptide Hydrolases; Peritoneal Neoplasms

In our attempts at establishing a cancer cell line from various ascites of cancer bearing patients, a cell line was successfully established from the ascites of a 63-year-old female with primary ovarian tumor (embryonal carcinoma). Histological findings of the peritoneum, due to metastasis, appeared to be cystadenocarcinoma, revealing the differentiation to non-epithelial cells which formed coarse networks and fibers, and morphologic changes of tissue cultures also reflected such histologic findings. At present the subculture has reached the 95th population doubling level, and cultured cells have assumed the morphology of mesothelial cells or fibroblasts with about 50 chromosomes. As a human malignant cell line, it is useful for the study of human malignant tumor cell.

Topics: Acid Phosphatase; Alkaline Phosphatase; Ascites; Autopsy; Basophils; Cell Line; Cell Transformation, Neoplastic; Chromosomes; Culture Techniques; Cytoplasm; Female; Humans; Middle Aged; Neoplasm Metastasis; Ovarian Neoplasms; Peritoneal Neoplasms; Phagocytosis; Teratoma

Topics: Acid Phosphatase; Animals; Ascitic Fluid; Catechol Oxidase; Female; Lymphocyte Activation; Lymphocytes; Lysosomes; Macrophages; Melanocytes; Melanoma; Mice; Mice, Inbred BALB C; Microscopy, Electron; Neoplasm Transplantation; Neoplasms, Experimental; Organoids; Peritoneal Neoplasms; Phagocytosis; Radiation Chimera; Spleen; Thymidine; Tritium

Topics: Acid Phosphatase; Adenocarcinoma; Ascites; Biopsy; Humans; Male; Middle Aged; Neoplasm Metastasis; Peritoneal Neoplasms; Prostatic Neoplasms; Radiography; Urinary Catheterization

Topics: Acid Phosphatase; Animals; Cytoplasmic Granules; Histocytochemistry; Mice; Microscopy, Fluorescence; Neoplasms, Experimental; Peritoneal Neoplasms; Peroxidases; Porphyrins; Staining and Labeling; Succinate Dehydrogenase