acid-phosphatase and Ovarian-Neoplasms

Topics: Acid Phosphatase; Blood Group Antigens; Carcinoembryonic Antigen; Female; Gastrointestinal Hormones; Humans; Immunoenzyme Techniques; Leukemia; Lymphoma; Male; Neoplasms; Ovarian Neoplasms; Pituitary Hormones; Pregnancy Proteins; Prostatic Neoplasms; Teratoma; Testicular Neoplasms; Thyroid Neoplasms

Prostatic acid phosphatase (ACPP) is a glycoprotein that is mainly synthesized and secreted by glandular epithelial cells (GE) of the prostate, and it is well known as a biomarker for prostate cancer. Although ACPP was used as prognostic/diagnostic indicator and studied to elucidate regulatory mechanism(s) during several decades in humans, its role is not clearly understood. Gene profiling data using a chicken DNA microarray revealed that ACPP increased significantly during remodeling and recrudescence of the oviduct in response to estrogen. Thus, in this study, we investigated the expression and hormonal regulation of ACPP gene in the reproductive tracts of chickens. ACPP was specifically detected in the luminal cells (LE) and GE of chicken oviduct, and diethylstilbestrol (a synthetic nonsteroidal estrogen) stimulated its expression during development of the oviduct. In addition, ACPP mRNA and protein were localized to LE and GE during the regeneration phase of the oviduct of laying hens during induced molting. Furthermore, ACPP mRNA and protein were abundant in GE of ovarian carcinoma, but not in normal ovaries. Moreover, strong expression of ACPP protein was detected in epithelial cells of cancerous ovaries from women. Collectively, results of the present study are the first to show that ACPP is a novel estrogen-stimulated gene in the oviductal epithelial cells of the chicken and that its expression increases significantly in epithelial cells of ovarian carcinoma, which indicates that it may be a candidate biomarker for diagnosis of epithelia-derived ovarian cancer in women.

Topics: Acid Phosphatase; Animals; Avian Proteins; Biomarkers, Tumor; Carcinoma; Chickens; Diethylstilbestrol; Female; Gene Expression Regulation; Humans; Ovarian Neoplasms; Oviducts; Protein Tyrosine Phosphatases

Male accessory sexual glands arising in ovarian cystic teratoma are exceedingly rare. We report a 56-year-old female subjected to an ovariohysterectomy due to a left ovarian mass. The pathological study of the surgical piece revealed a tumor composed of different mature tissue elements and well defined nodules of benign prostatic tissue.

Topics: Acid Phosphatase; Dermoid Cyst; Female; Humans; Male; Middle Aged; Ovarian Neoplasms; Prostate; Prostate-Specific Antigen; Protein Tyrosine Phosphatases; Teratoma

Protein-protein interactions and protein complex/aggregate formation play an essential role in almost all biological functions and activities. Through a nanoparticle aggregation immunoassay, we discovered that some proteins are substantially more complexed/aggregated in cancer tissues than normal tissues. This study examined four biomarkers proteins, CA125, CEA (carcinoembryonic antigen), CA19-9 and PAP (prostatic acid phosphatase) in ovarian, colon and prostate tissue lysates. The most exciting results were observed from the PAP assay of prostate tissues: prostate cancer can be clearly distinguished from normal prostate and prostate with benign conditions such as BPH (benign prostate hyperplasia) based on the complex/aggregation level of PAP in prostate tissue lysates. The complex/aggregate level of a protein can be potential biomarkers for cancer detection and diagnosis.

Topics: Acid Phosphatase; Adult; Aged; Aged, 80 and over; Antibodies; Biomarkers, Tumor; CA-125 Antigen; CA-19-9 Antigen; Carcinoembryonic Antigen; Colonic Neoplasms; Diagnosis, Differential; Female; Gold; Humans; Immunoassay; Male; Membrane Proteins; Metal Nanoparticles; Middle Aged; Neoplasms; Ovarian Neoplasms; Prostatic Hyperplasia; Prostatic Neoplasms; Protein Binding; Protein Conformation; Protein Tyrosine Phosphatases; Proteins; Sensitivity and Specificity

Mature cystic teratomas of the ovary containing prostatic remnants are reported in 2 women aged 31 and 20 years. Both cases showed the expected histology of mature teratomas with a mixture of ecto- and endodermal structures lying in a fibrous stroma. In both cases, the foci of prostate tissue were composed of typical prostatic glands arranged in acinar structures. One case displayed a transitional cell-lined duct resembling the urethra. Prostate glands showed intense positive immunostaining with prostatic specific antigen and prostatic acidic phosphatase. Focal images suggesting high-grade prostatic intraepithelial neoplasia were detected in 1 case. The literature on this unusual finding in these common tumors is reviewed and commented on.

Topics: Acid Phosphatase; Adult; Biomarkers, Tumor; Female; Humans; Male; Ovarian Neoplasms; Ovariectomy; Prostate; Prostate-Specific Antigen; Prostatic Intraepithelial Neoplasia; Prostatic Neoplasms; Protein Tyrosine Phosphatases; Teratoma; Treatment Outcome

We present an extremely rare case of a benign cystic ovarian teratoma with structures of male accessory sexual glands. The patient was a 30-year-old woman. A unilocular cystic tumor, measuring 5 cm in the largest diameter, was found in her right ovary and was removed. The teratoma contained epidermis, skin appendages, respiratory and intestinal epithelia, cartilage, muscle, and nervous and connective tissue. In addition to these histologically mature tissues, there were nodules with prostatic acini, prostate duct-like structures strongly positive for prostate-specific antigen and acid prostatic phosphatase, structures resembling Cowper's glands, and seminal vesicles surrounded by fibromuscular stroma. To our knowledge, this is the first case in the English literature describing seminal vesicles associated with prostatic tissue and bulbo-urethral glands in a mature ovarian teratoma.

Topics: Acid Phosphatase; Adult; Bulbourethral Glands; Female; Humans; Male; Ovarian Neoplasms; Prostate; Prostate-Specific Antigen; Protein Tyrosine Phosphatases; Seminal Vesicles; Teratoma; Treatment Outcome

Tartrate-resistant acid phosphatase (TRAP) is a metalloprotein enzyme that belongs to the acid phosphatases and is known to be expressed by osteoclasts. It has already been investigated as a marker of bone metastases in cancer patients. In this study, which examined the value of serum TRAP concentrations as a marker of bone disease in breast cancer patients, we observed high concentrations of TRAP even in patients without bone metastases. To elucidate this phenomenon, we examined the expression of TRAP in breast cancer cells and the cells of several other malignancies.. TRAP concentrations in the serum of tumor patients were determined by ELISA. The expression of TRAP in breast, ovarian, and cervical cancer and malignant melanoma was analyzed by immunohistochemistry. RT-PCR and immunocytology were used to evaluate TRAP expression in cultured tumor cells.. A marked increase in serum TRAP concentrations was observed in patients with breast and ovarian cancer, regardless of the presence or absence of bone disease. TRAP expression was found in breast and ovarian cancers and malignant melanoma, while cervical cancer showed only minimal expression of TRAP. Expression of TRAP was absent in benign tissue or was much less marked than in the corresponding malignant tissue. TRAP expression was also demonstrated in cultured primary cancer cells and in commercially available cell lines.. Overexpression of TRAP was detected in the cells of various different tumors. TRAP might be useful as a marker of progression of malignant disease. It could also be a potential target for future cancer therapies.

Topics: Acid Phosphatase; Breast Neoplasms; Control Groups; Enzyme-Linked Immunosorbent Assay; Female; Gene Expression; Humans; Immunohistochemistry; Isoenzymes; Melanoma; Ovarian Neoplasms; Reverse Transcriptase Polymerase Chain Reaction; Tartrate-Resistant Acid Phosphatase; Tumor Cells, Cultured; Up-Regulation

Adenocarcinomas may arise primarily from the urinary bladder, but secondary involvement from adenocarcinomas arising in adjacent organs is more common. In the present study we tried to differentiate primary urinary bladder adenocarcinomas from adenocarcinomas arising from the surrounding organs, based on their antigen profiles in routinely processed, paraffin-embedded tissue specimens. We analysed the staining results using stepwise linear discriminant analysis.. We investigated the usefulness of a panel of antibodies against cytokeratin 7, E48, cytokeratin 20, PSA, PSAP, CEA, vimentin, OC125 and HER-2/neu, to discriminate primary bladder adenocarcinoma from adenocarcinomas arising from the prostate, urachus, colon, cervix, ovary and endometrium. In the differential diagnosis with urinary bladder adenocarcinoma, an overall correct classification was reached for 77% and 81% of urachal and colonic carcinomas, respectively, using CEA, for 93% of prostatic adenocarcinomas using PSA, for 82% and 70% of cervical and ovarian adenocarcinomas, respectively, using OC125, and for 91% of endometrial adenocarcinomas using vimentin. Adding other antibodies did not improve the classification results for any of these differential diagnoses.. For the surgical pathologist, a panel of antibodies consisting of CEA, PSA, OC125 and vimentin is helpful to differentiate primary urinary bladder adenocarcinomas from adenocarcinomas originating from prostate and endometrium, less helpful in differentiation with urachal carcinoma, and not helpful in differentiation with colonic, cervical and ovarian carcinoma.

Topics: Abdominal Neoplasms; Acid Phosphatase; Adenocarcinoma; Antibodies, Monoclonal; Antibody Specificity; CA-125 Antigen; Carcinoembryonic Antigen; Carcinoma, Papillary; Cell Adhesion Molecules; Diagnosis, Differential; Endometrial Neoplasms; Female; Glycoproteins; GPI-Linked Proteins; Humans; Immunohistochemistry; Intermediate Filament Proteins; Keratin-20; Keratin-7; Keratins; Male; Neoplasms, Unknown Primary; Ovarian Neoplasms; Prostate; Prostate-Specific Antigen; Receptor, ErbB-2; Urachus; Urinary Bladder Neoplasms; Uterine Cervical Neoplasms; Vimentin

Circulating CD34+ cells were isolated from leukapheresis products collected from patients with ovarian cancer. CD34 contaminating cells, identified immediately after immunoselection, ranged from 5% to 25% in five different experiments and were predominantly CD3+ T-lymphocytes (range 2-12%), CD3+/CD16+/CD56+ natural killer cells (range 2-11%) and rare mature CD15+/ CD11b+ granulocytes (range 1-2%). CD34+ cells were cultured in liquid medium in the presence of interleukin-3, granulocyte-macrophage colony stimulating factor. stem cell factor, granulocyte colony stimulating factor and a powerful proliferation with prevalent differentiation along the granulocytic/monocytic lineage was obtained. After 10 d of culture a small but consistent number of early multinucleated osteoclasts were identified with a frequency of one cell per 700 granulocytic/monocytic cells, as revealed by cytologic examination. This observation was confirmed by staining for tartrate-resistant acid phosphatase activity which revealed red multinucleated elements with a frequency comparable to that reported above. Conversely, no osteoclasts were observed in those cultures in which macrophage overgrowth was obtained by culturing CD34+ cells until day 35. These observations suggest that circulating progenitors have a multilineage potential in vitro and contribute to the clarification of osteoclast development in humans: additionally, they provide the basis for the future development of optimized osteoclast culture techniques in liquid medium and the basic culture system, to test the distinct activity of 1,25(OH)2D3. parathyroid hormone interleukin-11 and of other cytokines on osteoclast development in humans.

Topics: Acid Phosphatase; Antigens, CD34; Cell Differentiation; Cell Lineage; Cells, Cultured; Female; Granulocyte-Macrophage Colony-Stimulating Factor; Hematopoietic Stem Cells; Humans; Interleukin-3; Isoenzymes; Osteoclasts; Ovarian Neoplasms; Stem Cell Factor; Tartrate-Resistant Acid Phosphatase

Strumal carcinoids (SC) are ovarian tumors containing thyroid parenchyma admixed with carcinoid elements. Microscopically, the carcinoid component of SC usually presents a ribbon or trabecular pattern similar to the pattern exhibited by hindgut carcinoids. The authors designed an immunohistochemical study to ascertain further similarities of the carcinoid component of SC to rectal carcinoids.. Five cases of SC were examined by light microscopic study with hematoxylin and eosin and immunoperoxidase staining with a group of neurohormonal peptides, thyroglobulin, prostatic acid phosphatase, and prostate specific antigen.. All tumors were positive for thyroglobulin, chromogranin, prostatic acid phosphatase (PAP), and glucagon. They were consistently negative for prostate specific antigen and variably positive to the other antibodies.. The consistent positivity of the carcinoid component of SC to PAP immunostaining is a further similarity of these tumors to rectal carcinoids, in which positive immunostaining to PAP has been observed. The similarity is not well understood, however, as all evidence points to the origin of SC from germ cells in mature cystic teratomas without any embryologic relationship to the hindgut.

Topics: Acid Phosphatase; Adolescent; Adult; Carcinoid Tumor; Chromogranins; Cystadenoma; Dermoid Cyst; Female; Glucagon; Humans; Immunoenzyme Techniques; Male; Middle Aged; Ovarian Neoplasms; Pancreatic Polypeptide; Prostate; Serotonin; Struma Ovarii; Thyroglobulin

A murine monoclonal antibody PASE/4LJ to prostatic acid phosphatase (PAP) was used to immunostain a wide variety of sections of benign and malignant tissues (654 blocks). Non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas, and scattered cells in prostatic and penile urethra were positive. Rat, dog and rabbit prostates were negative. Nine of 400 tumours of non-prostatic origin showed some positivity: 6/36 carcinoids, 1/9 islet cell tumours, 1/55 ovarian adenocarcinomas (serous) and one carcinosarcoma of the lung (epithelial portion). Positive staining was seen in islet cells in 4/5 specimens of normal pancreas, and in 4/9 blocks of normal pancreas surrounding a pancreatic tumour. Loops of Henle, maculae densae, and distal tubules in 10/10 fetal and 2/9 adult kidneys were also positive, with proximal tubules and collecting ducts negative. All other 159 blocks of non-neoplastic adult and fetal tissues were negative. The antibody was also affinity purified from ascitic fluid, and shown not to inhibit the enzyme activity of prostatic acid phosphatase.

Topics: Acid Phosphatase; Adult; Animals; Antibodies, Monoclonal; Dogs; Female; Humans; Immunohistochemistry; Kidney; Lung Neoplasms; Male; Ovarian Neoplasms; Prostate; Prostatic Neoplasms; Rabbits; Rats

Topics: 5'-Nucleotidase; Acid Phosphatase; AMP Deaminase; Carcinoma; Chromatography, Ion Exchange; Cyclic AMP; Female; Humans; Hydrolysis; Nucleotidases; Ovarian Neoplasms; Tissue Extracts

A series of six ovarian strumal carcinoids was examined. The presence of thyroid follicular epithelium was conclusively proved by the presence of follicular cells immunohistochemically positive for thyroglobulin, and by the ultrastructural identification of non-neuroendocrine cells with features of thyroid epithelium lining the follicles. Progressive replacement of thyroid epithelial cells by carcinoid cells accounts for the predominance of neuroendocrine granule-containing cells and the scarcity of thyroid epithelial cells lining many of the follicles. A variety of neuroendocrine hormones and other immunoreactive substances was demonstrated within carcinoid cells, including somatostatin (five cases), chromogranin (five cases), serotonin (five cases), glucagon (four cases), insulin (two cases), and gastrin (one case). Only one case contained calcitonin-positive cells. None were carcinoembryonic antigen-positive or had amyloid deposits. The carcinoid element in five cases stained positively for prostatic acid phosphatase. While strumal carcinoid shares some features with medullary carcinoma of the thyroid gland, it has sufficient differences to warrant a separate designation.

Topics: Acid Phosphatase; Adult; Aged; Calcitonin; Carcinoid Tumor; Epithelial Cells; Female; Histocytochemistry; Humans; Immunoenzyme Techniques; Middle Aged; Neurosecretory Systems; Ovarian Neoplasms; Struma Ovarii; Thyroglobulin; Thyroid Gland

Topics: Acid Phosphatase; Adult; Female; Humans; Isoenzymes; Kidney Neoplasms; Male; Middle Aged; Ovarian Neoplasms; Prostate; Prostatic Hyperplasia; Prostatic Neoplasms; Reference Values; Sex Factors; Urinary Bladder Neoplasms

In our attempts at establishing a cancer cell line from various ascites of cancer bearing patients, a cell line was successfully established from the ascites of a 63-year-old female with primary ovarian tumor (embryonal carcinoma). Histological findings of the peritoneum, due to metastasis, appeared to be cystadenocarcinoma, revealing the differentiation to non-epithelial cells which formed coarse networks and fibers, and morphologic changes of tissue cultures also reflected such histologic findings. At present the subculture has reached the 95th population doubling level, and cultured cells have assumed the morphology of mesothelial cells or fibroblasts with about 50 chromosomes. As a human malignant cell line, it is useful for the study of human malignant tumor cell.

Topics: Acid Phosphatase; Alkaline Phosphatase; Ascites; Autopsy; Basophils; Cell Line; Cell Transformation, Neoplastic; Chromosomes; Culture Techniques; Cytoplasm; Female; Humans; Middle Aged; Neoplasm Metastasis; Ovarian Neoplasms; Peritoneal Neoplasms; Phagocytosis; Teratoma

Topics: Acid Phosphatase; Biopsy; Diagnosis, Differential; Female; Humans; Lymph Nodes; Male; Methods; Ovarian Neoplasms; Prostate; Prostatic Neoplasms; Staining and Labeling

Topics: Acid Phosphatase; Alkaline Phosphatase; Cystadenoma; Enzymes; Female; Humans; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Ovarian Cysts; Ovarian Follicle; Ovarian Neoplasms; Ovum

Topics: Acid Phosphatase; Alkaline Phosphatase; Aminopeptidases; Carbohydrates; Culture Techniques; Cyclophosphamide; Female; Histocytochemistry; Humans; Ovarian Neoplasms; Ovary; Oxidoreductases

Topics: Acid Phosphatase; Adenocarcinoma; Adenocarcinoma, Mucinous; Alkaline Phosphatase; Connective Tissue; Endometriosis; Female; Histocytochemistry; Humans; Ovarian Neoplasms; Phosphoric Monoester Hydrolases

Topics: Acid Phosphatase; Alkaline Phosphatase; Cyclophosphamide; Female; Humans; Neoplasm Metastasis; Omentum; Ovarian Neoplasms; Phosphoric Monoester Hydrolases

Topics: Acid Phosphatase; Alkaline Phosphatase; Aspartate Aminotransferases; Carcinoma; Female; Humans; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Ovarian Cysts; Ovarian Neoplasms

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Androgens; Biopsy; Collagen; Esterases; Female; Glycosaminoglycans; Gonadotropins, Pituitary; Humans; Hydroxysteroid Dehydrogenases; Hypothalamus; L-Lactate Dehydrogenase; Leiomyoma; Microscopy, Electron; Ovarian Neoplasms; Ovary; Polycystic Ovary Syndrome; Uterine Neoplasms

Topics: Acid Phosphatase; Adenocarcinoma, Papillary; Alanine; Alkaline Phosphatase; Amides; Amidohydrolases; Arginine; Brain; Carcinoma, Intraductal, Noninfiltrating; Cecal Neoplasms; Cobalt; Dipeptidases; Female; Glioma; Humans; Leucine; Manganese; Naphthalenes; Ovarian Neoplasms; Peptide Hydrolases; Phenylalanine

Topics: Acid Phosphatase; Adult; Alanine Transaminase; Alcohol Oxidoreductases; Alkaline Phosphatase; Aspartate Aminotransferases; Cholinesterases; Cyclophosphamide; Female; Glucose-6-Phosphate Isomerase; Glutamate Dehydrogenase; Humans; Hydroxybutyrate Dehydrogenase; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Lipase; Ovarian Neoplasms; Phosphoric Monoester Hydrolases; Prognosis; Transaminases; Trypsin Inhibitors

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Bone Neoplasms; Clinical Enzyme Tests; Diagnosis, Differential; Female; Humans; L-Lactate Dehydrogenase; Liver Neoplasms; Middle Aged; Neoplasm Metastasis; Ovarian Neoplasms; Transaminases

Topics: Acid Phosphatase; Animals; Brain Neoplasms; Breast Neoplasms; Esterases; Female; Granulation Tissue; Granuloma, Giant Cell; Histocytochemistry; Hodgkin Disease; Humans; Hydrolases; Leucyl Aminopeptidase; Neoplasms; Ovarian Neoplasms; Oxidoreductases; Rats

Topics: Acid Phosphatase; Adenocarcinoma; Alanine Transaminase; Alkaline Phosphatase; Aspartate Aminotransferases; Bone Neoplasms; Clinical Enzyme Tests; Dysgerminoma; Female; Fructose-Bisphosphate Aldolase; Glutathione; Hexoses; Humans; Isocitrate Dehydrogenase; Isomerases; Kidney Neoplasms; L-Lactate Dehydrogenase; Liver Function Tests; Liver Neoplasms; Malate Dehydrogenase; Male; Neoplasm Metastasis; Ovarian Neoplasms; Oxidoreductases; Prostatic Neoplasms; Transaminases; Ureteral Neoplasms; Urinary Bladder Neoplasms

Topics: 5'-Nucleotidase; Acid Phosphatase; Alkaline Phosphatase; Coloring Agents; Dysgerminoma; Female; Humans; Male; Ovarian Neoplasms; Phosphoric Monoester Hydrolases; Protein Tyrosine Phosphatases; Seminoma; Testicular Neoplasms; Testis