acid-phosphatase and Otosclerosis

"Considerable interest has been raised in recent years concerning the basic mechanisms involved in bone destruction and rebuilding in the otospongiotic focus. Under general bone resorption the osteoclasts play a decisive role, but in otospongiotic tissue electron-microscopic and cytochemical studies have shown that osteoclasts alone are not responsible for the bone resorption. Mononuclear histiocytes found in the marrow spaces and in the surrounding bone of an otospongiotic focus together with osteocytes take active part in the resorption. Cytochemical studies of acid phosphatase activity have shown that hydrolases in the lysosomes are expelled into the surrounding tissue, resulting in its resorption.

Topics: Acid Phosphatase; Bone Resorption; Humans; Osteoclasts; Otosclerosis; Temporal Bone

Mucosal biopsy specimens were taken from the middle ear in 19 patients with otosclerosis and stained by Alcan blue and PAS. They were also stained for hydrolytic and oxidative enzymes. In additon, 11 temporal bones from patients without any previous ear disease were serially sectioned for mucopolysaccharides. In otosclerosis, both acid and neutral mucopolysaccharides were present in the mucosa, but not to the same extent as in the temporal bone specimens. Acid phosphatase, lactate malate dehydrogenases, and nonspecific esterase were demonstrated even in very thin epithelium, providing a potential basis for the appearance of these enzymes in large amounts in various inflammatory middle ear diseases.

Topics: Acid Phosphatase; Ear, Middle; Esterases; Glycosaminoglycans; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Malate Dehydrogenase; Mucous Membrane; Otosclerosis; Temporal Bone

Topics: Acid Phosphatase; Animals; Bone Neoplasms; Ear, Inner; Fluorides; Guinea Pigs; Histocytochemistry; Injections; Organ of Corti; Otosclerosis; Sodium; Staining and Labeling

Topics: Acid Phosphatase; Humans; Lysosomes; Microscopy, Electron; Otosclerosis

Topics: Acid Phosphatase; Adult; Aged; Biopsy; Cartilage; Collagen; Connective Tissue; Ear, Inner; Histiocytes; Humans; Middle Aged; Osteoblasts; Osteocytes; Otosclerosis; Stapes Surgery

Topics: Acid Phosphatase; Biopsy; Bone Resorption; Cell Nucleus; Histiocytes; Humans; Lysosomes; Microscopy, Electron; Osteoclasts; Otosclerosis; Pseudopodia

Topics: Acid Phosphatase; Alkaline Phosphatase; Bone Resorption; Cytoplasm; Ear, Middle; Female; Histocytochemistry; Humans; In Vitro Techniques; Male; Microscopy, Electron; Mucous Membrane; Osteocytes; Otosclerosis; Phosphoric Monoester Hydrolases

Topics: Acid Phosphatase; Bone Resorption; Capillaries; Collagen Diseases; Decalcification Technique; Histological Techniques; Humans; Lysosomes; Methods; Microscopy, Electron; Osteoclasts; Osteocytes; Otosclerosis; Stapes Surgery

Topics: Acid Phosphatase; Alkaline Phosphatase; Aspartate Aminotransferases; Ear Ossicles; Fructose-Bisphosphate Aldolase; Glucosephosphate Dehydrogenase; Humans; L-Lactate Dehydrogenase; Malate Dehydrogenase; Otosclerosis; Oxidoreductases; Proteins

Topics: Acid Phosphatase; Alkaline Phosphatase; Clinical Enzyme Tests; Electrophoresis; Esterases; Haptoglobins; Humans; Otosclerosis