acid-phosphatase and Otitis-Media-with-Effusion

The role of pharyngeal lymphoid tissue in etiopathogenesis of secretory otitis is not yet defined. The influence of tonsillar and adenoid mass, weight, obstruction of naspharyngeal orrifitium, bacterial reservoire or some immunological events are of scientific interest. Tissue nonspecific alkaline phosphatase (TNAP) and acid phosphatase (ACP) are enzymes detected in lymphoid tissue, TNAP as characteristic of B cells, ACP as a characteristic of macrophages and folucullardentritic cells. These enzymes interfere in cell metabolism by removing 5' phosphate group from nucleotides and proteins. Specific activity and kinetic properties were studied in palatinal tonsils and adenoids of children with secretory otitis (OME) and compared with children with recurrent tonsillitis without ear involvement.. Adenoid and tonsillar tissue of l7 children with OME and 30 children with recurrent tonsillitis were subjected to biochemical investigation using method of releasing of p-nitrophenol from p-nitrophenylphosphate (pNPP). Kinetic parameters as Michaelis-Menten constant were calculated by non-linear regression estimation method.. Specific activity of adenoid alkaline phosphatase was lower in children with OME in relation to children with recurrent tonsillitis (t=5.733507, p<0.01). Specific activity of adenoid acid phosphatase was also lower in children with OME (t=3.655456, p<0.01). pH optimum for both enzymes was the same in these two groups of children. Michaelis-Menten constant for both enzymes was significantly higher in adenoid of children with OME than in children with recurrent tonsillitis suggesting lower enzyme affinity for the substrate.. Differences in specific activities and kinetic properties of adenoid alkaline and acid phosphatases between children with OME and children with recurrent tonsillitis without OME were verified in this study. The results of the study are not able to explain the alteration of alkaline and acid phosphatase characteristics but could point to some possible and specific role of nasopharyngeal lymphoid tissue in pathogenesis of secretary otitis.

Topics: Acid Phosphatase; Adenoidectomy; Adenoids; Alkaline Phosphatase; B-Lymphocytes; Child; Child, Preschool; Dendritic Cells, Follicular; Female; Humans; Hydrogen-Ion Concentration; Indicators and Reagents; Macrophages; Male; Nasal Obstruction; Nitrophenols; Organophosphorus Compounds; Otitis Media with Effusion; Palatine Tonsil; Recurrence; Tonsillectomy; Tonsillitis

This study describes the localization of antigen-presenting cells (APC) in the different compartments in adenoids of children with otitis media with effusion (OME) and "healthy" children and adults. It is shown that the adenoid of children with OME contains a relatively high number of OKT6 and RFD1 positive cells. Moreover, accumulations of these cells are present in the extrafollicular areas of these adenoids. Very occasionally dendritic cells in the epithelial microenvironment contain Birbeck granules, indicating characteristic Langerhans cells. These OKT6 positive cells are not seen in the adenoids of the control group. Our results clearly show a relation between the presence of dendritic cells and the occurrence of OME. No differences are seen in localization and morphology of the APC in the studied adenoids.

Topics: Acid Phosphatase; Adenoids; Antibodies, Monoclonal; Antigen-Presenting Cells; B-Lymphocytes; Child; Child, Preschool; Dendritic Cells; Female; Histocytochemistry; Humans; Immunohistochemistry; Infant; Male; Microscopy, Electron; Otitis Media with Effusion

Two Chinese patients with sinus histiocytosis and massive lymphadenopathy are reported. The results of enzyme and immunohistochemical studies are presented.

Topics: Acid Phosphatase; Adult; alpha 1-Antitrypsin; Asian People; Biopsy; Child; China; Esterases; Female; Histiocytes; Humans; Immunoenzyme Techniques; Immunoglobulins; Lymph Nodes; Lymphatic Diseases; Male; Muramidase; Otitis Media with Effusion; S100 Proteins

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Arylsulfatases; Cathepsin D; Child; Child, Preschool; Exudates and Transudates; Humans; Lysosomes; Middle Aged; Otitis Media; Otitis Media with Effusion

Aural polypi associated with chronic suppurative otitis media have been studied histopathologically and histochemically in 20 patients. The polypi consist of an edematous connective tissue stroma infiltrated by chronic inflammatory cells and numerous blood vessels. The surface is covered by intact stratified squamous epithelium. The histochemical study revealed altered permeability of the blood vessels which seem to be the main pathological background for polyp formation. An enhanced phagocytic activity and increased metabolic activity were found in the epithelium in the chronic inflammatory cells. No glandular activity was found in the polypi.

Topics: Acid Phosphatase; Alkaline Phosphatase; Chronic Disease; Ear Neoplasms; Humans; Otitis Media with Effusion; Polyps; Succinate Dehydrogenase

Topics: Acid Phosphatase; Alanine Transaminase; Alkaline Phosphatase; Aspartate Aminotransferases; Biopsy; D-Alanine Transaminase; Histocytochemistry; Otitis Media with Effusion; Suppuration