acid-phosphatase and Osteomyelitis

To investigate the histopathologic study of the ethmoid bone in chronic sinusitis (CRS) and the role of bone pathology in the pathogenesis of CRS.. Specimens of mucosa of ethmoid sinus with bone were collected during operation from 40 patients with CRS, 25 males and 15 females, aged 44.8, and specimens of mucosa of ethmoid sinus were collected from 16 patients with other otorhinolaryngologic diseases, 9 males and 7 females, aged 40.9 during operation. The specimens underwent HE, tartrate-resistant acid phosphatase, alkaline phosphatase, and Van Gieson staining so as to obtain the scores of nasal mucosa inflammation, pathology of mucoperiosteum and bone, and activity of bone remodeling.. The average score of mucosal inflammation of the CRS group was 2.30 +/- 0.88, significantly higher than that of the control group (1.38 +/- 0.81, P = 0.004). The score of mucoperiosteum and bone pathology of the CRS group was 1.65 +/- 0.84, significantly higher than that of the control group (1.00 +/- 0.73, P = 0.004). The score of bone remodeling activity of the CRS group was 2.15 +/- 0.74, significantly higher than that of the control group (1.56 +/- 0.63, P = 0.007). In the CRS patients, the score of mucosal inflammation was not significantly correlated with the mucoperiosteum and bone pathology (R = 0.047, P = 0.772), and the activity of bone remodeling (R = 0.021, P = 0.897).. The ethmoid bone of CRS patients shows marked fibrosis, bone remodeling, structure of woven bone, and pathologic changes similar to those of chronic osteomyelitis, especially bone absorption, new bone formation, etc.

Topics: Acid Phosphatase; Adult; Aged; Alkaline Phosphatase; Chronic Disease; Ethmoid Bone; Female; Histocytochemistry; Humans; Isoenzymes; Male; Middle Aged; Nasal Bone; Nasal Mucosa; Osteomyelitis; Sinusitis; Tartrate-Resistant Acid Phosphatase

The determination of the level of leukocytes and ESR in 25 patients was shown to be of low informative value in differential diagnostics of destructive injuries of feet in patients with diabetes mellitus. An analysis of the biochemical markers of the bone remodeling has demonstrated high informative value of this method in differential diagnostics of acute stage of diabetic osteoarthropathy and osteomyelitis that allows to use it in complex examinations of osteomyelitis suspects.

Topics: Acid Phosphatase; Acute Disease; Adult; Diabetes Complications; Diagnosis, Differential; Female; Humans; Isoenzymes; Male; Middle Aged; Osteoarthropathy, Secondary Hypertrophic; Osteomyelitis; Radiography; Tartrate-Resistant Acid Phosphatase

We investigated the origin of multinuclear cells (MNCs) in the granulation tissue in osteomyelitis by immunohistochemical techniques in 66 patients. 12 samples were analyzed for the presence of CD68, cathepsin K, CD11b and tartrate-resistant acid phosphatase (TRAP) activity. Many MNCs were present in the granulation tissue adjacent to a sequestrum. MNCs in contact with the sequestrum were also noted, however, no osteoblasts were found. Immunohistochemically, CD68, cathepsin K and TRAP were strongly expressed in most of the MNCs, while CD11b positive cells were not found. MNCs remote from and in contact with the sequestrum showed the same immunohistochemical features which are characteristic of osteoclasts. Further, MNCs in contact with the sequestrum had originally developed in the granulation tissue and directly infiltrated towards the sequestrum without cell-to-cell interaction with osteoblasts.

Topics: Acid Phosphatase; Adult; Aged; Antigens, CD; Antigens, Differentiation, Myelomonocytic; Cathepsin K; Cathepsins; Cell Count; Cell Differentiation; Child; Female; Giant Cells; Granulation Tissue; Humans; Immunohistochemistry; Immunophenotyping; Isoenzymes; Macrophage-1 Antigen; Male; Middle Aged; Osteoclasts; Osteomyelitis; Tartrate-Resistant Acid Phosphatase

An examination of 46 patients has shown that the enzymatic status of circulating leukocytes of blood is a highly informative criterium which allows to judge about the pyo-inflammatory process, its spread, degree, reactivity of the patient's organism, efficiency of the therapy performed, prognosis of the disease.

Topics: Acid Phosphatase; Acute Disease; Adolescent; Alkaline Phosphatase; Child; Child, Preschool; Glycerolphosphate Dehydrogenase; Humans; Leukocytes; Osteomyelitis; Peroxidases; Succinate Dehydrogenase

Topics: Acid Phosphatase; Acute Disease; Adolescent; Adult; Alkaline Phosphatase; Female; Glycogen; Humans; Jaw Diseases; Male; Middle Aged; Neutrophils; Osteomyelitis; Periostitis; Peroxidase; Phagocytosis

Topics: Acid Phosphatase; Child; Child, Preschool; Histocytochemistry; Humans; Infections; Male; Neutrophils; Osteomyelitis

Enzyme activity of lactate dehydrogenase, glutamate-oxalacetate and glutamate-pyruvate transaminase, creatine phosphokinase, cholinesterase, alkaline, acid and prostatic phosphatase and aldolase has been studied in a total of 213 subjects, of whom 97 were of good health, 63 had bone tumors and 53 suffered from osteomyelitis. The activities of the majority of the enzymes were found to become significantly changed in comparison with the norm. In both patient groups, the more striking differences being noted in that of osteomyelitis. However, enzymatic activity alone does not allow to differentiate the group of bone tumors from that of osteomyelitis, the differences between these two groups not being of significance in any one of the enzymes followed.

Topics: Acid Phosphatase; Alanine Transaminase; Alkaline Phosphatase; Aspartate Aminotransferases; Bone Neoplasms; Cholinesterases; Creatine Kinase; Fructose-Bisphosphate Aldolase; Humans; L-Lactate Dehydrogenase; Osteomyelitis; Phosphoric Monoester Hydrolases

Topics: Abdominal Neoplasms; Acid Phosphatase; Acute Disease; Aminosalicylic Acid; B-Lymphocytes; Child; Diagnosis, Differential; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Leukocytes; Mediastinal Neoplasms; Osteomyelitis; Paralysis; Rheumatic Diseases; Sepsis; T-Lymphocytes

Topics: Acid Phosphatase; Acute Disease; Alkaline Phosphatase; Bacterial Infections; Carbon Isotopes; Citric Acid Cycle; Glucose; Glucosephosphate Dehydrogenase; Glutathione Reductase; Humans; Lupus Erythematosus, Systemic; Neoplasms; Neutrophils; Osteomyelitis; Oxygen Consumption; Peroxidases; Phosphogluconate Dehydrogenase; Pyruvate Kinase; Tetrazolium Salts; Tuberculosis, Pulmonary; Virus Diseases

Topics: Acid Phosphatase; Alkaline Phosphatase; Amyloid; Animals; Histocytochemistry; Kidney; Liver; Lung; Lysosomes; Mice; Microscopy, Electron; Neutrophils; Osteomyelitis; Phagocytosis; Spleen