acid-phosphatase and Orthomyxoviridae-Infections

Infection of chick embryo cell (CEC) cultures with influenza virus results in the appearance of neuraminidase activity lost by the cells as a result of cultivation. Neuraminidase activity is associated mainly with the lysomal cell fraction. Different distribution of neuraminidase activity in lysosomes with various densities, different reaction to sodium ethylene diamine tetracetate (EDTA) and differenct optimal pH suggest that at early stages of viral infection the cell enzyme is activated and by the 7th hour of infection viral neuraminidase is synthesized.

Topics: Acid Phosphatase; Animals; Cells, Cultured; Chick Embryo; Edetic Acid; Hydrogen-Ion Concentration; Lysosomes; Mitochondria; Neuraminidase; Orthomyxoviridae; Orthomyxoviridae Infections; Virus Cultivation

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Cells, Cultured; Galactosidases; Glycoside Hydrolases; Haplorhini; Hexosaminidases; Kidney; L Cells; Mannose; Orthomyxoviridae; Orthomyxoviridae Infections; Time Factors

Topics: Acid Phosphatase; Animals; Carbon Isotopes; Chick Embryo; Chloroquine; Culture Media; Fibroblasts; Leucine; Lysosomes; Newcastle disease virus; Orthomyxoviridae Infections; Protein Biosynthesis; Proteins; RNA; Time Factors; Tritium; Uridine; Virus Replication

Topics: Acid Phosphatase; Aerosols; Air Pollution; Alkaline Phosphatase; Animals; Environmental Exposure; Germ-Free Life; Histocytochemistry; Lung; Mice; Nitrogen Dioxide; Orthomyxoviridae Infections; Oxygen Consumption; Staphylococcal Infections