acid-phosphatase and Nephrosis

To explore the effect of Modified Sijunzi Decoction (MSD) on the bone metabolism of prednisone intervened adriamycin-induced nephropathy rats.. The adriamycin-induced nephropathy rat model was prepared. Totally 50 SD rats were randomly divide into five groups, i.e., the model group, the hormone group, the Chinese medicine (CM) group, the CM + hormone group, and the normal control group. The 24-h urine samples were collected on the 7th, 21st, and 35th day after modeling. The 24-h urine protein was measured by biuret colorimetry. Serum levels of osteoprotegerin (OPG), receptor activator of nuclear factor-kappaB ligand (RANKL), osteocalcin (BGP), and tartrate-resistant acid phosphatase (TRACP) were determined by ELISA. Expressions of OPG and RANKL in the tibia tissue were detected using real-time quantitative PCR and Western blot.. (1) Compared with the normal control group, the 24-h urine protein increased in each group on the 7th, 21st, and 35th day (P < 0.05, P < 0.01). Compared with the model group, the 24-h urinary protein decreased in the hormone group and the CM + hormone group (P < 0.05, P < 0.01). The decrement was more obvious along with the treatment time went by (P < 0.05, P < 0.01). There was statistical difference in the reduction of urine protein on the 35th day between the CM group and the model group (P < 0.05). (2) Compared with the 21st-day of the same group, the serum levels of TRACP and RANKL increased (P < 0.05, P < 0.01). Compared with the model group, the serum levels of the TRACP and RANKL increased (P < 0.05, P < 0.01), OPG and BGP decreased (P < 0.05, P < 0.01) in the hormone group. Compared with the CM group at the same period, serum OPG level decreased and the RANKL level increased in the hormone group and the CM + hormone group (P < 0.05, P < 0.01). Besides, the serum level of TRACP increased and BGP decreased (P < 0.05, P < 0.01). Compared with the hormone group at the same period, OPG and BGP increased (P < 0.05, P < 0.01), RANKL decreased (P < 0.01) in the CM + hormone group. On the 35th day TRACP decreased (P < 0.01). (3) Compared with the normal group, mRNA expressions of OPG and RANKL on the 21st day increased (P < 0.05, P < 0.01), mRNA expressions of OPG and RANKL on the 35th day decreased in the model group (P < 0.01). Compared with the CM group at the same period, OPG mRNA expression decreased (P < 0.01) and RANKL mRNA expression increased in the hormone group (P < 0.05). OPG mRNA expression decreased in the CM +hormone group (P < 0.05). (4) Compared with the hormone group on the 21st day, the OPG level decreased and the RANKL protein increased (both P < 0.05). RANKL decreased in the CM + hormone group (P < 0.05). Compared with the model group at the same period, OPG decreased and RANKL increased in the hormone group (P < 0.01). Compared with the CM group at the same period, OPG decreased (P < 0.01), RANKL increased (P < 0.01) in the hormone group and the CM + hormone group. Compared with the hormone group at the same period, OPG increased and RANKL decreased in the CM + hormone group (both P < 0.01).. Prednisone could induce osteoporosis through the OPG/RANKL/RANK pathway. MSZ could slow down the formation of prednisone-induced osteoporosis through promoting osteoblast differentiation, and inhibiting osteoclastogenesis.

Topics: Acid Phosphatase; Animals; Doxorubicin; Drugs, Chinese Herbal; Isoenzymes; Male; Nephrosis; Osteocalcin; Osteoprotegerin; Prednisone; RANK Ligand; Rats; Rats, Sprague-Dawley; Tartrate-Resistant Acid Phosphatase; Tibia

Topics: Acetylglucosaminidase; Acid Phosphatase; Alkaline Phosphatase; Animals; Diuresis; Galactosidases; Hexosaminidases; Kidney; Male; Nephrosis; Proteinuria; Puromycin; Rats; Ribonucleosides; Urea

Topics: Acid Phosphatase; Acute Kidney Injury; Alkaline Phosphatase; Animals; beta-Aminoethyl Isothiourea; Calcinosis; Depression, Chemical; Electron Transport Complex IV; Histocytochemistry; Injections, Intraperitoneal; Kidney; Kidney Tubular Necrosis, Acute; Kidney Tubules, Proximal; Loop of Henle; Male; Nephrosclerosis; Nephrosis; Rats; Rats, Inbred Strains; Succinate Dehydrogenase; Time Factors

Topics: Acid Phosphatase; Adult; Amino Acids; Animals; Cytoplasm; Epithelial Cells; Glomerulonephritis; Golgi Apparatus; Humans; Kidney; Kidney Glomerulus; Kidney Tubules, Proximal; Lysosomes; Male; Microscopy, Electron; Mitochondria; Morphogenesis; Nephrons; Nephrosis; Peroxidases; Protein Biosynthesis

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adolescent; Adult; Aged; Glomerulonephritis; Humans; Kidney Failure, Chronic; Middle Aged; Nephrosis; Proteinuria

Topics: Acid Phosphatase; Adolescent; Adult; Biopsy; Child; Glomerulonephritis; Glucosephosphate Dehydrogenase; Histocytochemistry; Humans; Isocitrate Dehydrogenase; Kidney; Kidney Glomerulus; Kidney Tubules; L-Lactate Dehydrogenase; Nephrosis; Nephrotic Syndrome

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Glutamate Dehydrogenase; Kidney; Nephrosis; Rats; Succinate Dehydrogenase; Vibration

Topics: Acid Phosphatase; Adenosine Triphosphatases; Animals; Carbon Isotopes; Centrifugation, Density Gradient; Electron Transport Complex IV; Hypertonic Solutions; Injections, Intravenous; Kidney; Kidney Cortex; Kidney Tubules, Proximal; Lysosomes; Male; Nephrosis; Osmotic Pressure; Pinocytosis; Rats; Sucrose

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Cytoplasmic Granules; Esterases; Kidney; Male; Microscopy, Fluorescence; Nephrosis; Osmosis; Oxidoreductases; Phosphotransferases; Rats; Sucrose; Time Factors

Topics: Acid Phosphatase; Alkaline Phosphatase; Analysis of Variance; Animals; Autoradiography; Female; Histocytochemistry; Kidney Tubules; Nephrosis; Nucleosides; Puromycin; Rats; Thymidine; Tritium

Topics: Acid Phosphatase; Animals; Bilirubin; Cholestasis; Glucuronidase; Glutamates; Kidney; Lysosomes; Male; Mitochondria; Nephrosis; Rats; Succinates; Time Factors

Topics: Acid Phosphatase; Animals; Chlorides; Citric Acid Cycle; Histocytochemistry; Kidney; Kidney Tubules; L-Lactate Dehydrogenase; Male; Mercury; Methods; Nephrosis; Rats

Topics: Acid Phosphatase; Animals; Esterases; Kidney; Lysosomes; Male; Microscopy; Microscopy, Electron; Nephrosis; Pinocytosis; Rats; Sucrose

Topics: Acid Phosphatase; Adenosine Triphosphatases; Cathepsins; Electron Transport Complex II; Electron Transport Complex IV; Endopeptidases; Histological Techniques; Malate Dehydrogenase; Metabolism; Mitochondria; Nephrosis; Nucleosides; Phosphorus; Poisons; Puromycin Aminonucleoside; Rats; Research; Succinate Dehydrogenase; Tissue Culture Techniques; Toxicology; Uranium

The digestive cycle following reabsorption of hemoglobin by cells of the proximal convoluted tubules in mouse kidney and the uptake of ferritin by glomerular mesangial cells in the kidney of normal and nephrotic rats were investigated by electron microscopical histochemical procedures. Mouse kidneys, sampled at closely spaced time points between 1 to 48 hours after intraperitoneal injection of hemoglobin, and rat (normal and nephrotic) kidneys, sampled at 30 minutes, 2 hours, and 48 hours after intravenous injection of ferritin, were fixed in glutaraldehyde, cut at 50 micro on a freezing microtome, incubated for acid phosphatase and thiolacetate-esterase, and postfixed in OsO(4). Satisfactory preservation of fine structure permitted the localization of the enzymatic reaction products on cell structures involved in uptake and digestion of exogenous proteins. The latter were identified either by their density (hemoglobin) or their molecular structure (ferritin). It was found that lysosomal enzymic activities and incorporated exogenous proteins occur together in the same membrane-bounded structures. In the cells of the proximal convolution, lytic activities become demonstrable within 1 hour after hemoglobin injection, appear first in apical vacuoles filled with hemoglobin, and persist in fully formed protein absorption droplets. At the end of the lytic cycle ( approximately 48 hours post injection), the cells have an increased population of polymorphic bodies which exhibit lytic activities. In smaller numbers, identical bodies occur in controls. It is concluded that they represent remnants of previous digestive events. The means by which the resorptive vacuoles acquire hydrolytic activities remain unknown. Fusion of newly formed vacuoles with residual bodies was not seen, and hemoglobin incorporation into such bodies was only occasionally encountered. Acid phosphatase activity was found sometimes in the Golgi complex, but enzyme transport from the complex to the resorbing vacuoles could not be established. Autolytic vacuoles containing mitochondria or mitochondrial remnants were frequently found during the early stages of hemoglobin resorption, but no definite conclusions about the mechanism involved in the segregation of endogenous material were obtained. In nephrotic rats ferritin was segregated in membrane-bounded bodies mainly in the mesangial cells and to a lesser extent in epithelial and endothelial cells. Most of these sites were marked by the reactio

Topics: Acid Phosphatase; Aldehydes; Animals; Biological Transport; Cell Membrane; Cytoplasm; Electrons; Esterases; Ferritins; Golgi Apparatus; Hemoglobins; Hemoglobinuria; Histocytochemistry; Kidney; Kidney Glomerulus; Kidney Tubules; Lysosomes; Mice; Microscopy; Microscopy, Electron; Mitochondria; Nephrosis; Proteins; Rats; Research; Vacuoles

Topics: Acid Phosphatase; Animals; Cytoplasm; Electrons; Esterases; Ferritins; Hemoglobins; Histocytochemistry; Kidney; Lysosomes; Mice; Microscopy; Microscopy, Electron; Nephrosis; Rats; Research