acid-phosphatase and Nephritis

There are conflicting data regarding the clinical benefit of the effect of HMG-CoA reductase inhibitors (statins) in osteoporosis. We have reported that fluvastatin (a statin) is effective in improving proteinuria and renal function in childhood IgA nephropathy with mild histological findings and moderate proteinuria. The aim of the present study was to clarify the effect of fluvastatin on the bone mineral density, bone metabolic markers, proteinuria, and renal function of children with minimal change glomerulonephritis with some focal mesangial cell proliferation whose glomeruli did not stain positive for IgA and on moderate proteinuria.. We conducted a prospective controlled study of 36 children who had recently been diagnosed with normocholesterolemic minimal change glomerulonephritis with some focal mesangial cell proliferation and moderate proteinuria, and in whom strenuous exercise was restricted. The 36 patients were randomly assigned to receive 20 mg of fluvastatin (group 1) or 5 mg/kg of dipyridamole (group 2) for two years.. By the end of the trial, there was no difference in BMD between the groups, and there were no changes in the four bone metabolic parameters. However, the urinary protein, hematuria and BUN levels had significantly decreased in group 1 compared to baseline, and the serum total protein and albumin levels and creatinine clearance had significantly increased in group 1 compared to baseline and group 2.. The results of this study suggest that fluvastatin therapy has an antiproteinuric effect and improves renal function in moderately proteinuric patients with mild histological glomerulonephritis, but does not increase BMD.

Topics: Acid Phosphatase; Adolescent; Adult; Alkaline Phosphatase; Amino Acids; Bone Density; Child; Fatty Acids, Monounsaturated; Female; Fluvastatin; Humans; Hydroxymethylglutaryl-CoA Reductase Inhibitors; Indoles; Isoenzymes; Male; Nephritis; Nephrosis, Lipoid; Osteocalcin; Prospective Studies; Tartrate-Resistant Acid Phosphatase

Unspecific inflammatory lesions of the urinary tract are known to be a vital question in urology and nephrology of our country and in foreign ones. Used in experiments were rats of mixed population. Lysosomal enzymes were studied in homogenates of the liver and contralateral kidney tissues and in the blood serum as well 1, 3, 6 days after simulation of the pathology experimental model. Activities were measured of such lysosomal enzymes as acid DNA-ase and RNA-ase, cathepsin, and acid phosphatase. Results of the studies made showed that in unspecific purulent affection of the kidneys there occurs an activation of acid blood hydrolases followed by their activation in the liver. The contralateral kidney, because of it being linked in functioning with the liver, remains relatively "inert" with respect to activation of lysosomal enzymes.

Topics: Acid Phosphatase; Animals; Cathepsin D; Deoxyribonucleases; Disease Models, Animal; Female; Kidney; Liver; Lysosomes; Male; Nephritis; Rats; Ribonucleases; Suppuration; Time Factors

Topics: Acid Phosphatase; Adolescent; Alkaline Phosphatase; beta-Galactosidase; Child; Child, Preschool; gamma-Glutamyltransferase; Hematuria; Humans; L-Lactate Dehydrogenase; Nephritis

Using a modified model of Masugi's nephritis of rats, various enzymatic activities in urine, serum and renal tissue (glomeruli or cortex) were determined at appropriate intervals after the administration of anti-kidney serum and compared with the urinary protein content and the kidney weight. In the urine, alkaline phosphatase (Al-Phosase), acid phosphatase (Ac-Phosase) and N-acetyl-beta-glucosaminidase (NA-beta-Gase) activities remarkably increased after the induction of nephritis, reached their peaks on the 10th day and reverted to almost the normal levels on the 30th day. The patterns of time course of these enzymatic activities were similar to patterns seen in the urinary protein content and the kidney weight. In the serum, the Al-Phosase activity decreased slightly, while NA-beta-Gase activity increased slightly. The Ac-Phosase activity in serum remained at normal levels during the experimental periods. In the glomeruli, the bound activities of these three enzymes decreased with nephritis, showing a negative correlation with results in the urine. On the other hand, fibrinolytic activities in the urine (plasmin-like enzyme) and renal cortex (plasminogen activator) also paralleled the urinary protein content and the kidney weight in the course of the disease. These results suggest that the Al-Phosase, Ac-Phosase and NA-beta-Gase excreted into urine in cases of nephritis may be mostly derived from damaged renal cells and one part of Al-Phosase may also come from the plasma. Moreover, the increase of plasmin-like enzyme in urine is considered to be due to the increase of plasminogen activator in the renal cortex. Thus, the determination of these enzymatic activities in the urine should be useful for evaluating effects of drugs for the treatment of nephritis.

Topics: Acetylglucosaminidase; Acid Phosphatase; Alkaline Phosphatase; Animals; Hexosaminidases; Immune Sera; Kidney; Kidney Cortex; Kidney Glomerulus; Male; Nephritis; Organ Size; Rats

The enzymatic activity of blood leukocytes was studied in rats with Masugi nephritis by means of the quantitative cytochemical method. Nephrotoxin injection during first hours and days did not produce specific changes in the enzymatic activity. Maximum changes were observed on the 8-th day when the elevation of the activity of dehydrogenase lymphocytes and neutrophils acid phosphatase was observed at the same time with most pronounced histological changes in the kidneys, the decrease of the complement titer and the presence of nephrotoxin and autoglobulin in the renal glomeruli. Later the histological changes became less pronounced, and the enzymatic activity decreased. In rats with proteinuria the activity of the neutrophil leukocytes acid phosphatase was higher than in animals without proteinuria.

Topics: Acid Phosphatase; Animals; Antibodies; Fluorescent Antibody Technique; Glycerolphosphate Dehydrogenase; Immune Sera; Kidney; Leukocytes; Lymphocytes; Nephritis; Neutrophils; Rats; Succinate Dehydrogenase; Time Factors

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Aspartate Aminotransferases; Chlorides; Galactosidases; Glomerulonephritis; Immune Sera; Isoenzymes; Kidney Tubules; L-Lactate Dehydrogenase; Mercury; Nephritis; Polyuria; Proteinuria; Rats

Topics: Acid Phosphatase; Adolescent; Adult; Age Factors; Child; Child, Preschool; Female; Humans; Hydronephrosis; Infant; Kidney; Kidney Calculi; Kidney Diseases; Kidney Neoplasms; Male; Nephritis; Sex Factors; Wilms Tumor

Topics: Acid Phosphatase; Alkaline Phosphatase; Arthritis, Rheumatoid; Collagen Diseases; Dermatomyositis; Humans; Lupus Erythematosus, Systemic; Nephritis; Neutrophils; Peroxidases; Prednisolone

Topics: Acid Phosphatase; Albuminuria; Animals; Cathepsins; Deoxyribonucleases; Glucuronidase; Kidney Diseases; Male; Mercury Poisoning; Microscopy, Electron; Nephritis; Rabbits; Ribonucleases

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alanine Transaminase; Alkaline Phosphatase; Aspartate Aminotransferases; Biomedical Research; Bronchial Spasm; D-Alanine Transaminase; Humans; Hypertension; Hypertension, Renal; Kidney; Nephritis; Phosphorus Metabolism Disorders