acid-phosphatase and Neoplasm-Regression--Spontaneous

Topics: Acid Phosphatase; Adenocarcinoma; Animals; Cathepsins; Estrone; Female; Glucuronidase; Histocytochemistry; Lysosomes; Mammary Neoplasms, Experimental; Neoplasm Regression, Spontaneous; Rats

Rat mammary tumours induced by 7,12-dimethylbenz[a]anthracene can undergo repeated growth and regression during successive pregnancies. In a 10-day period after birth about half of the tumours regressed 50% or more. The concentrations of the lysosomal enzymes increased in regressing mammary tumours to the following multiples of the initial values: beta-glucuronidase, 7.7; beta-galactosidase, 3.9; cathepsin, 2.9; acid ribonuclease, 2.1; arylsulphatase A, 1.5; acid phosphatase, 1.4. In contrast, several non-lysosomal enzymes failed to increase. Activities in the post-partum uterus increased to the following multiples of the initial values: beta-glucuronidase, 5.8; cathepsin, 5.5; acid ribonuclease, 4.3; beta-galactosidase, 2.2; acid phosphatase, 1.8. Arylsulphatase A in the post-partum uterus decreased significantly, suggesting a non-lysosomal distribution or a special function related to pregnancy. No other significant changes were observed in the lysosomal or non-lysosomal enzymes in the hormone-independent liver or hormone-dependent normal mammary gland. The ratio of free to bound arylsulphatase A and acid ribonuclease decreased slightly 1-3 days after birth because of problems in homogenizing the tumours. At days 4-8, however, there was a dramatic increase in the ratio of the free to bound activities. The results can be explained in terms of the lysosomal theory of intracellular digestion.

Topics: Acid Phosphatase; Animals; Benz(a)Anthracenes; Cathepsins; Female; Galactosidases; Glucuronidase; Liver; Lysosomes; Mammary Glands, Animal; Mammary Neoplasms, Experimental; Neoplasm Regression, Spontaneous; Pregnancy; Pregnancy, Animal; Rats; Ribonucleases; Sulfatases; Uterus

Topics: Acid Phosphatase; Alkaline Phosphatase; Animals; Benz(a)Anthracenes; Estradiol; Female; Glucuronidase; Growth; Histocytochemistry; Mammary Neoplasms, Experimental; Neoplasm Regression, Spontaneous; Ovary; Rats; Ribonucleases

Topics: Acid Phosphatase; Animals; Cathepsins; Female; Galactosidases; Necrosis; Neoplasm Regression, Spontaneous; Neoplasm Transplantation; Rats; Sarcoma, Experimental; Sulfatases; Transplantation, Homologous