acid-phosphatase and Nasopharyngeal-Neoplasms

Cytologic assay of metastatic involvement of lymph nodes may be used along with histological studies as a reliable adjunct for morphological diagnosis, that makes possible in many cases to determine not only the epithelial origin of the affection but also to characterize its histological pattern. However, the cytologic diagnosis of undifferentiated cancer metastases is frequently rather difficult in relation to establishing the differential diagnosis between the former and other affections of lymph nodes. A careful, and sometimes repeated analysis of the material, taking into account the clinical data and cytochemical findings, contributes to greater efficiency of a cytologic method. The perspective use of cytochemical reactions as additional criteria for the diagnosis of undifferentiated cancer necessitates their further elaboration and study.

Topics: Acid Phosphatase; Enzyme Activation; Esterases; Humans; Lung Neoplasms; Lymph Nodes; Lymphatic Metastasis; Mouth Neoplasms; Nasopharyngeal Neoplasms

A series of blood samples from more than 200 histologically confirmed Chinese patients with NPC in Singapore were typed for 25 genetically controlled red-cell enzyme and five serum protein systems. A comparable number of patients suspected of having NPC but histologically negative and a series of healthy unrelated Chinese were typed for the same systems. The gene frequencies of NPC patients and controls differed by 4% or more in four of the 11 systems that showed variation; a further system, G6PD deficiency, also showed a significant difference between the two series but was excluded because of possible unreliability of the results from patients. Smaller differences existed in several other systems, including chromosome 6 markers closely linked to HLA. An analysis of differences within dialect groups showed a consistent effect for PGD, but for red-cell acid phosphatase there was a reversal of the difference between patients and controls among the Cantonese. These results need a larger series to confirm their validity. A breakdown of patients into those 30 years of age or older and those under 30 slightly enhanced the differences in gene frequencies. A multivariate analysis, using genetic distance statistics, showed a significant difference between NPC patients and controls, which is evident also when they are compared in the separate dialect groups. The histologically negative patients occupied an intermediate position. The study indicates that etiological factors resulting in clinically and histologically confirmed NPC operate on a genetically distinct subpopulation of Chinese in Singapore.

Topics: Acid Phosphatase; Adolescent; Adult; Age Factors; Aged; Carcinoma; Child; China; Chromosome Mapping; Chromosomes, Human, 6-12 and X; Erythrocytes; Gene Frequency; Genes; Glucosephosphate Dehydrogenase; Humans; Middle Aged; Nasopharyngeal Neoplasms; Phenotype; Risk; Singapore

There were carried out substrate- and enzymhistochemical reactions of the typical nasopharyngeal angiofibroma in 6 cases. In the environs of the vessels there were mainly neutral mucopolysaccharides, in the left intercellular substance and in the fibroblasts and most cells acid carbohydrates were found. The protein reactions pointed out a conspicouos strong contents of alkaline proteins in fibroblasts, mast cells and vessel environs. In the mast cells and fibroblasts tryptophan was found plentifully, in fibroblasts and vessel environs a lot of tyrosin was provable. The alakline phosphatase was traceable in fibroblasts and mast cells with great intensity. Strong reactivities of the acid phosphatases in all of the cells of the tissue refer to intensive lysosomal activities and considerable secretion transactions. The reaction of LDH as a representative of energy metabolism settles down in all of the cells and vessel environs in a strong way. The histochemical examination of a preparation of the naso-pharyngeal angiofibroma after hormone therapy showed a falling out of all the enzyme activities. The reduction of the acid phosphatases seems to be of special importance. Substrathistochemically there weren't any clear modifications concerning quality and quantity. A comparatively examined separation of an ordinary fibroma of the nasopharynx indicated similar enzymhistochemical pictures disregard the intensive reaction of the unspecific esterase, which doesn't appear concerning the nasopharyngeal angiofibroma. The carbohydrate reactions demonstrated some differences to the typical nasopharyngeal angiofibroma.

Topics: Acid Phosphatase; Alkaline Phosphatase; Glycosaminoglycans; Histiocytoma, Benign Fibrous; Histocytochemistry; Hormones; Humans; L-Lactate Dehydrogenase; Nasopharyngeal Neoplasms; Neoplasm Proteins

Topics: Acid Phosphatase; Aminopeptidases; Amnion; Carcinoma; Cathepsins; Cell Line; Cycloheximide; Dactinomycin; Dexamethasone; Enzyme Induction; Glucocorticoids; HeLa Cells; Humans; Hydrocortisone; Isoenzymes; Kinetics; Lung; Lysosomes; Methods; Naphthalenes; Nasopharyngeal Neoplasms; Prednisolone; Skin

Topics: Acid Phosphatase; Adenocarcinoma; Biopsy; Carcinoma, Bronchogenic; Carcinoma, Squamous Cell; Colorimetry; Esophageal Neoplasms; Female; Gastrointestinal Neoplasms; Hodgkin Disease; Humans; Intestinal Neoplasms; Laryngeal Neoplasms; Leucyl Aminopeptidase; Leukemia; Liver Neoplasms; Lymphoma, Non-Hodgkin; Male; Melanoma; Nasopharyngeal Neoplasms; Neoplasms; Pancreatic Neoplasms; Prostatic Neoplasms; Tongue Neoplasms; Urogenital Neoplasms