acid-phosphatase and Myositis

Topics: Acid Phosphatase; Alanine-tRNA Ligase; Autoantibodies; Cyclophosphamide; Drug Therapy, Combination; Glucocorticoids; Humans; Male; Middle Aged; Muscle, Skeletal; Myositis; Pain; Prednisolone; Treatment Outcome

Five cases of nodular fasciitis, three cases of proliferative fasciitis, and one case of proliferative myositis were investigated by tartrate-resistant acid phosphatase (TRAP) histochemistry on conventional paraffin-embedded material. In all cases varying numbers of enzyme-positive cells were detected. Cytological investigations revealed the presence of small round cells, elongated spindle cells, medium-sized mono- or binucleated cells, and multinuclear giant cells, the latter resembling osteoclasts. However, the typical ganglion-like cells were TRAP-negative in almost all tests. The TRAP-pattern may help to differentiate nodular fasciitis and allied lesions from inflammatory pseudotumours or organising haematomas, as the latter do not contain TRAP-positive cells. As TRAP appears to be a marker for a special population of phagocytizing and non phagocytizing histiocytes, nodular fasciitis and its proliferative variants may be assumed to be fibrohistiocytic tumorous conditions.

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Fasciitis; Female; Histiocytes; Histocytochemistry; Humans; Immunoenzyme Techniques; Male; Middle Aged; Myositis; Phagocytosis

On the basis of previous (n = 23) and current muscle biopsies (n = 25) as well as five animal-experimental investigations, the paper deals with the cause and the manifestation of perifascicular muscle-fiber atrophy. A deteriorated trophic situation of the subfascial or marginal muscle fibers in patients suffering from immunocomplex vasculopathies in connection with a reduced supply of blood to the muscles is seen as the cause of the areactive weight loss (atrophy). This is accompanied by a considerably reduced capillary supply of blood to the muscle fibers. Even in normal conditions, the portion of collateral vessels and capillaries is lower in the subfascial region than in the center of the fascicle. When the blood supply is disturbed, the marginal fibers are in a trophic situation worse than that of the central muscle fibers. They become atrophied. In responsive patients, the muscle fibers regenerate and recapillarize during the convalescence period. This can be shown histochemically by means of the alkaline phosphatase reaction. The extent of perifascicular musclefiber atrophy can be fixed in quantitative terms by the method of Baumli and Mumenthaler. Where a second biopsy is indicated, results can be obtained regarding the changes in the perifascicular atrophy as a consequence of the therapy provided.

Topics: Acid Phosphatase; Adolescent; Adult; Aged; Animals; Basement Membrane; Biopsy; Capillaries; Child; Dermatomyositis; Female; Fluorescent Antibody Technique; Humans; Immune Complex Diseases; Immunoglobulins; Ischemia; Male; Middle Aged; Muscle, Smooth, Vascular; Muscles; Muscular Atrophy; Muscular Dystrophies; Myositis; Rats; Rats, Inbred Strains

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adult; Alkaline Phosphatase; Amyotrophic Lateral Sclerosis; Biopsy; Child, Preschool; Dihydrolipoamide Dehydrogenase; Esterases; Glucosyltransferases; Glucuronidase; Glycerolphosphate Dehydrogenase; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Muscle Denervation; Muscles; Muscular Atrophy; Muscular Dystrophies; Myositis; Myotonic Dystrophy; NAD; Neuromuscular Diseases; Succinate Dehydrogenase

Topics: Acetates; Acid Phosphatase; Adolescent; Adult; Child; Child, Preschool; Dermatomyositis; Glycoside Hydrolases; Hexosaminidases; Humans; Middle Aged; Muscles; Muscular Diseases; Muscular Dystrophies; Myositis; Myotonic Dystrophy; Surface-Active Agents

Topics: Acid Phosphatase; Adult; Aged; Biopsy; Female; Histocytochemistry; Humans; L-Lactate Dehydrogenase; Male; Middle Aged; Muscles; Muscular Dystrophies; Myositis; Necrosis; Oxidoreductases; Phagocytosis; Regeneration; RNA; Sarcolemma; Succinate Dehydrogenase

Topics: Acid Phosphatase; Aminopeptidases; Animals; Esterases; Golgi Apparatus; Lysosomes; Myositis; Rats; Thiamine Pyrophosphate; Trichinellosis