acid-phosphatase and Lymphopenia

Topics: Acid Phosphatase; Adult; Bone Diseases; Cerebrosides; Epilepsy, Temporal Lobe; Gaucher Disease; Graft Rejection; Graft vs Host Reaction; Humans; Intellectual Disability; Kyphosis; Lymphopenia; Male; Postoperative Complications; Radionuclide Imaging; Spleen; Technetium; Thrombocytopenia; Transplantation, Homologous

To evaluate a possible mechanism of stress-induced lymphopenic effect we assessed the activity of lymphocyte lysosomal enzymes (LE) under immobilization. The effects of immobilization stress on LE (AP, acid phosphatase, cathepsin D and L, beta-N-acetyl-glucosamidase) activity in lymphocytes, number of lymphocytes and plasma cortisol (COR) level in the peripheral blood were examined in the cross-bred Pietrain pigs showing genotypic (presence or lack of RyR1 gene mutation) and phenotypic (reactivity to halothane) differences. It was found that immobilization stress evoked an increase in LE which was concomitant with lymphopenia and a rise of COR level. The most pronounced enhancement of LE, which may reflect a tendency to lymphocyte cytolysis, was found in the recessive homozygotes RyR1 (nn) phenotypically defined as stress/halothane susceptible as well as in the heterozygotes RyR1 (Nn) included in the group of stress/halothane resistant. Despite this individual variability the stress-induced increase in LE activity was present in all the animals. It seems that a possibility of destruction (lysis) of lymphocyte cells should not be excluded as one of the causes of stress lymphopenia.

Topics: Acetylglucosaminidase; Acid Phosphatase; Animals; Cathepsin D; Cathepsin L; Cathepsins; Cysteine Endopeptidases; Genetic Predisposition to Disease; Genotype; Hydrocortisone; Lymphocyte Count; Lymphocytes; Lymphopenia; Lysosomes; Male; Restraint, Physical; Stress, Physiological; Swine

In rats chronically exposed to benzene vapours of 1200 mg/m3 concentration, 6 hr daily, 6 days a week, during 12 weeks, acid phosphatase activity (AcP) was evaluated in neutrophils and lymphocytes of peripheral blood. One found an increase in AcP activity in neutrophils, correlating with duration of exposure, lymphocytopenia, correlating with duration of exposure, and increase in AcP--positive lymphocytes percentage, mainly with cytoplasmic and lysosomocytoplasmic localization of enzyme. Besides distant effects of chronic benzene intoxication were evaluated, 5 months after the exposure had been intermitted. Normal AcP activity in neutrophils, lymphocytopenia, and increase in AcP--positive lymphocytes percentage were found with cytoplasmic and lysosomo-cytoplasmic localization of enzyme. The authors suggest a possibility of using the changes observed in lymphocytes, on a subcellular level, to evaluate distant effects of occupational exposure to benzene.

Topics: Acid Phosphatase; Animals; Benzene; Leukocytes; Lymphocytes; Lymphopenia; Lysosomes; Male; Rats; Time Factors

Topics: Acid Phosphatase; Adult; Humans; Lymphocytes; Lymphopenia; Male; Middle Aged; Occupational Diseases; Paint

Topics: Acid Phosphatase; Animals; Benzene; Lymphocytes; Lymphopenia; Male; Rats; Selenium