acid-phosphatase and Lymphoma--Large-B-Cell--Diffuse

Topics: Acid Phosphatase; Age Factors; Antibodies, Monoclonal; Diagnosis, Differential; Humans; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Microscopy, Electron; Naphthol AS D Esterase; Protease Inhibitors; Rosette Formation; Sex Factors

Osteolysis and hypercalcemia are observed in 5-15%, and 10%, respectively, of malignant lymphoma patients during their clinical course. However, both osteolysis and hypercalcemia are uncommon at onset of the disease. We encountered a 24-year-old male non-Hodgkin's lymphoma patient who had multiple osteolytic lesion from the onset of the disease and repeated episodes of hypercalcemia during the clinical course. The patient died with refractory disease. We studied the expression of chemokines which might affect bone resorption using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. Increased expressions of MIP-1alpha, MIP-1beta and RANKL, which are osteoclast-activating factors, were observed in the RNA derived from the patient's lymphoma cells. The secretion of osteoclast-activating factors such as MIP-1alpha by the tumor cells (and/or bone marrow stromal cells) might be involved in the etiology of osteolysis and hypercalcemia in some malignant lymphoma cases.

Topics: Acid Phosphatase; Adult; Biopsy; Bone Marrow Cells; Bone Resorption; Carrier Proteins; Chemokine CCL3; Chemokine CCL4; Chemokines; Fatal Outcome; Humans; Hypercalcemia; Immunophenotyping; Isoenzymes; Low Back Pain; Lymphoma, B-Cell; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Macrophage Inflammatory Proteins; Magnetic Resonance Imaging; Male; Membrane Glycoproteins; Osteoclasts; Osteolysis; RANK Ligand; Receptor Activator of Nuclear Factor-kappa B; Reverse Transcriptase Polymerase Chain Reaction; Tartrate-Resistant Acid Phosphatase

Intravascular large B-cell lymphoma (IVL) has been treated as fever of unknown origin (FUO), and many patients have been treated inadequately based on incorrect diagnoses. We previously cares for a patient with IVL who tested positive for prostatic acid phosphatase (PAP), a marker of prostate cancer. Since then, we have regularly examined this mather when IVL was suspected to investigate the usefulness of PAP as a diagnostic marker for IVL. We retrospectively evaluated the usefulness of PAP as diagnostic marker of IVL.. We reviewed the clinical courses of 5 patients with IVL (3 males, 2 females) in comparison with 23 controls with hematologic malignancies other than IVL.. Serum levels of PAP were elevated in all 5 patients with IVL and 2 of the 23 controls. The difference was statistically significant using a chi-squared test (p=0.0002). The sensitivity and specificity of PAP were 100% and 91%, respectively, in the diagnosis of IVL. Its serum levels were closely associated with disease status.. This study suggests that PAP might be a useful marker for the screening and assessment of disease activity and responses to the treatment of IVL.

Topics: Acid Phosphatase; Aged; Biomarkers, Tumor; Case-Control Studies; Clinical Enzyme Tests; Female; Humans; Lymphoma, B-Cell; Lymphoma, Large B-Cell, Diffuse; Male; Middle Aged; Protein Tyrosine Phosphatases; Retrospective Studies; Vascular Neoplasms

Intravascular large B-cell lymphoma (LBCL) is a rare and aggressive subtype of diffuse LBCL characterized by disseminated intravascular proliferation of neoplastic lymphocytes. Obstruction of blood flow by tumor cells in a variety of organs can cause an array of clinical changes, including alteration of the neural and spinal system and the respiratory system, as well as skin lesions. It is usually very difficult to diagnose intravascular LBCL in a patient simply from clinical symptoms or laboratory examinations. We here document our findings that serum prostatic acid phosphatase levels in both males and a female (2.2-24.0 microg/L) reflect the presence of intravascular LBCL, changing synchronously in response to chemotherapy. To determine whether prostatic acid phosphatase (PAP) might be a useful tumor marker for early diagnosis, we reviewed five intravascular LBCLs. Immunohistochemically, tumor cells in all cases were positive for anti-PAP antibody. The results were further confirmed in one case by Western-blot analysis and in another by the detection of amplified messenger RNA for PAP in microdissected tumor cells, respectively. PAP has not been detected in 17 lymphomas (diffuse LBCL, 8 cases; follicular lymphoma, 3 cases; T-cell lymphoma, 3 cases; Hodgkin lymphoma, 3 cases) by Western blot analyses. We conclude that serum PAP is a useful tumor marker for intravascular LBCL and that it deserves further investigation in this context.

Topics: Acid Phosphatase; Biomarkers, Tumor; Blotting, Western; Dissection; Female; Humans; Immunohistochemistry; Laser Therapy; Leukocyte Common Antigens; Lymphoma, B-Cell; Lymphoma, Large B-Cell, Diffuse; Male; Middle Aged; Protein Tyrosine Phosphatases; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Vascular Neoplasms

This report describes six cases of feline large granular lymphocyte lymphoma identified by light microscopy on the basis of their characteristic azurophilic granulation in Giemsa-stained plastic sections and by electron microscopy on the basis of their typical granules. Although the granules of all the tumor cells were negative for peroxidase activity, they all demonstrated chloroacetate-esterase and acid phosphatase activity. All the tumors reacted with cross-reacting antibodies against the CD3 antigen (epsilon chain) and did not react with a cross-reacting monoclonal antibody directed against epitopes on cytoplasmic domains of the CD20 antigen. Three tumors had a positive reaction with a monoclonal human CD57-like antibody. This is highly suggestive of either a cytotoxic T cell or a natural killer cell origin of the neoplasias. In three cats, although other abdominal organs were affected to a variable extent, the main neoplastic lesions were localized in the gastrointestinal tract and the jejunal lymph nodes. In contrast, in the other three cats, organ involvement was more widespread, affecting the lung (two), myocardium (two), precardiac mediastinum (one), salivary gland (one), and spinal cord (one); in addition, leukemia was present in two of these cats. The data presented indicate that tumors made up of large granular lymphocytes occur more frequently in cats than previously assumed and that they share many characteristic features with specific subtypes of clonal disorders of large granular lymphocytes in humans.

Topics: Acid Phosphatase; Animals; Carboxylic Ester Hydrolases; Cat Diseases; Cats; CD3 Complex; CD57 Antigens; Cytoplasmic Granules; Female; Immunoenzyme Techniques; Leukemia, Lymphoid; Lymphoma, Large B-Cell, Diffuse; Male; Retrospective Studies

A well-demarcated solitary splenic mass (20 x 20 x 15 cm in size) containing hemorrhagic and necrotic foci was observed in a 4-year-old Thoroughbred stallion. Histologically, the mass consisted of lymphoma cells of the diffuse large non-cleaved type, with a high mitotic index and scattered macrophages that formed a starry sky pattern. The lymphoma cells revealed diffuse positivity for acid phosphatase and alpha naphthyl butyrate esterase, and were also positive for intracytoplasmic IgM on occasion, and mostly for proliferating cell nuclear antigen. Ultrastructural examination revealed moderately-developed rough endoplasmic reticulum sometimes with dilated cisternae. Thus, the diagnosis was a primary splenic lymphoma of B cell origin, but the exact reason for the absence of invasive growth or metastasis despite the high proliferative activity of this neoplasm was unclear.

Topics: Acid Phosphatase; Animals; Carboxylic Ester Hydrolases; Horse Diseases; Horses; Lymphoma, B-Cell; Lymphoma, Large B-Cell, Diffuse; Male; Microscopy, Electron; Mitotic Index; Splenic Neoplasms

Lymph node sinuses consist mainly of sinus lining cells with clear dendritic morphology. Enzyme cytochemical and immune phenotypical analysis classify sinus lining cells as an intermediate cell type linking monocyte/macrophage to follicular dendritic cells, which are accepted immune accessory cells to humoral immune response. Labeling studies indicate sinus lining cells to hematogenous and possible monocytogenous in origin. In functional term sinus lining cells bind and present soluble antigen as the first cell type following antigen pulse. In addition sinus lining cells bind carcinoma cells and may play a role in metastasis. Sinus lining cells give rise to a well defined entity of neoplasia which is proposed to be termed sinus lining cell reticulosarcoma. In this presentation a case report exemplifies such a new entity.

Topics: Acid Phosphatase; Alkaline Phosphatase; Carboxylesterase; Carboxylic Ester Hydrolases; Histocytochemistry; Humans; Immunohistochemistry; Lymph Nodes; Lymphoma, Large B-Cell, Diffuse; Peroxidase

1. The cell bound glycosidases in sublines and clones of the histiocytic cell line U-937 have previously been shown to display characteristic patterns. 2. In this paper the effects of differentiation inducing agents upon glycosidase patterns of one subline, U-937 GTB, are presented. 3. Teleocidin, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), dimethyl-sulfoxide (DMSO), dihydroxyvitamin D3 and supernatants from mixed lymphocyte culture (MCL) all induce cellular differentiation of U-937 GTB. 4. Significant changes of the levels of cell bound glycosidases were seen after addition of inducing agents. 5. Alterations have been monitored as relative effects upon the absolute glycosidase activities and as effects upon selected ratios of different glycosidases. 6. The separate inducing agents show distinct enzyme patterns.

Topics: Acid Phosphatase; Calcitriol; Cell Differentiation; Cell Line; Clone Cells; Dimethyl Sulfoxide; Enzyme Induction; Glycoside Hydrolases; Humans; Hydrogen-Ion Concentration; Lymphoma, Large B-Cell, Diffuse; Lyngbya Toxins; Phosphates; Tetradecanoylphorbol Acetate

1. The activities of nine glycosidases, lysozyme and acid and alkaline phosphatases were compared in the histiocytic lymphoma cell line U-937 and a set of clones and sublines derived from this line. 2. The patterns of the different enzyme activities and selected enzyme ratios have been used as a method to distinguish between different clones and sublines. 3. Sublines with high lysozyme levels were rich in most cell-bound glycosidases. 4. During long-term growth distinct enzyme patterns of individual lines were preserved. 5. The enzyme pattern during a cell culture growth cycle was basically stable.

Topics: Acid Phosphatase; Alkaline Phosphatase; Cell Division; Clone Cells; Glycoside Hydrolases; Humans; Lymphoma, Large B-Cell, Diffuse; Muramidase; Time Factors; Tumor Cells, Cultured

The morphological, enzyme- and immunohistochemical features of a sarcoma arising from interdigitating reticulum cells (IDRC) are presented. These cells are normal constituents of the T-dependent region of lymphoid organs, and their function is largely unresolved. The immunohistochemical findings in the present case indicate that neoplastic IDRC are morphologically and phenotypically similar to normal IDRC in lymphoid organs. Functionally however, neoplastic IDRC more closely resemble IDRC in the fetal lymph node and in the thymus, where they are thought to play a role in the final differentiation of immature thymocytes into mature peripheral T-lymphocytes.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Aged; Antibodies, Monoclonal; Female; Histocytochemistry; Humans; Immunoenzyme Techniques; Lymphatic Diseases; Lymphoid Tissue; Lymphoma, Large B-Cell, Diffuse; Microscopy, Electron

Tartrate-resistant acid phosphatase (TRAcP) is used as a marker for osteoclasts, which are believed to be derived from phagocytic cells or phagocyte stem cell precursors. To further investigate the relationship between monocytic phagocytes and osteoclasts, acid phosphatase (AcP) activity was measured by three different techniques in human peripheral blood monocytes, monocyte-derived macrophages, and the U937 cell line. We found that cytochemistry and gel electrophoresis led to similar results, but that the colorimetric assay was inconsistent. Normal human peripheral monocytes expressed both tartrate-sensitive and -resistant AcP. In culture these cells formed polykaryons and expressed TRAcP activity that was further identified as an isoenzyme associated with bone tissue. In contrast, the U937 cells did not express TRAcP activity as measured by gel electrophoresis. Both U937 cells and monocytes possess material that interferes with interpretation of the colorimetric assay of AcP. The presence of TRAcP in monocyte-derived macrophages further supports the relationship between phagocytic cells and bone osteoclasts.

Topics: Acid Phosphatase; Calcitriol; Cell Line; Colorimetry; Histocytochemistry; Humans; Isoenzymes; Lymphoma, Large B-Cell, Diffuse; Macrophages; Monocytes; Tartrates

Since the advent of the newer classifications of non-Hodgkin's lymphoma and the realization that the majority of tumors classified as histiocytic under the Rappaport classification were in fact of lymphocytic origin, there have been remarkably few reports of true histiocytic (monocyte/macrophage) tumors and it has been suggested by some that such tumors should not be considered as a variety of malignant lymphoma. This article describes five patients with malignant lymphoma of neither B- or T-lymphocyte origin in whom the malignant cells could be characterised immunologically, cytochemically, and immunohistochemically as of true histiocytic derivation. The cases showed considerable morphologic diversity but there were shared characteristics at both light microscopic and ultrastructural levels. Positive immunohistochemical staining for alpha 1-antitrypsin was the single most useful criterion in classifying these tumors. Without the use of special techniques there were no clinical or pathologic features that reliably distinguished these cases from non-Hodgkin's lymphomas of lymphocytic derivation. Tumors of histiocytic origin are, therefore, inevitably being included among the non-Hodgkin's lymphomas and are most appropriately classified as such. Identification of histiocytic lymphomas should be encouraged so prognosis and optimum treatment can be established.

Topics: Acid Phosphatase; Aged; alpha 1-Antitrypsin; Esterases; Histocytochemistry; Humans; Immunoglobulin Fc Fragments; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Male; Microscopy, Electron; Middle Aged; Receptors, Fc; Rosette Formation

Human myeloid leukemia cell lines ML-1, ML-2, ML-3, promyelocytic leukemia cell line HL-60 and histiocytic lymphoma cell line U-937 were induced to differentiate by 0.5-10 ng/ml (0.8-16 nM) 12-O-tetradecanoylphorbol-13-acetate (TPA). After 48-72 h of induction, changes of the morphology, cytochemistry and of the antigenic phenotype of induced and control cells were studied using a panel of monoclonal antibodies against granulocytic, monocytic, HLA-ABC and HLA-DR antigens in indirect immunofluorescence. Cells of the TPA-treated cultures acquired morphological, cytochemical and antigenic markers of monocytes/macrophages, as surface adherence, alpha-naphthyl acetate esterase (alpha-NE) and acid phosphatase activity and the expression of monocytic antigens detected with monoclonal antibodies 63D3, FMC 17, B 44.1, B 52.1 and anti-Mol. During differentiation in vitro induced by TPA, also loss of HLA-DR antigens and diminution of antigen of cell activation were detected with antibodies L 243 and 4F2. The expression of granulocytic antigens was only slightly diminished and the expression of HLA-ABC antigens was not changed by TPA-treatment. There were differences in the percentage of cells induced to differentiate among the lines of different origin and even among the lines ML-1, ML-2 and ML-3, established from a single patient with acute myeloid leukemia. After treatment of cultures with 5 ng/ml TPA for 72 h DNA synthesis was inhibited to 60-80%.

Topics: Acid Phosphatase; Antibodies, Monoclonal; Antigens, Neoplasm; Carboxylic Ester Hydrolases; Cell Differentiation; Cell Line; Fluorescent Antibody Technique; Histocompatibility Antigens Class II; Histocytochemistry; HLA Antigens; HLA-A Antigens; HLA-DR Antigens; Humans; Leukemia, Myeloid; Lymphoma, Large B-Cell, Diffuse; Naphthol AS D Esterase; Neoplasms, Experimental; Tetradecanoylphorbol Acetate

The cytomorphology and lysosomal enzyme activity of the haemopoietic and reticuloendothelial system (RES) of SJL/J mice, which develop a spontaneous reticulum cell neoplasm type B (RCN-B), were studied. The cytological findings during development of the disease were a predominance of medium-sized lymphocytes and an increase in the number of hyperbasophilic cells and plasma cells in the RES. These pathological findings were compared to the changes due to ageing in control groups of healthy C3H/eB mice. Acid phosphatase activity increased in the RES, whereas that of beta-D-glucuronidase remained unchanged during development of the disease. These findings indicate that the RCN-B of the SJL/J mice bear a certain resemblance to Hodgkin disease in man.

Topics: Acid Phosphatase; Animals; Bone Marrow; Cell Transformation, Neoplastic; Female; Glucuronidase; Hematopoietic Stem Cells; Leukopenia; Lymph Nodes; Lymphoma, Large B-Cell, Diffuse; Lysosomes; Male; Mice; Mice, Inbred C3H; Mice, Inbred Strains; Mononuclear Phagocyte System; Spleen; Thymus Gland

The significance of histo- and cytochemical enzyme investigations in the diagnosis of malignant Non-Hodgkin's lymphomas (NHL) is evaluated. Histochemical enzyme methods complete the morphological diagnosis if some general principles of diagnostic histochemistry are observed. This is particularly true for the diagnosis of the hairy cell leukemia, the T-lymphoblastoma and the histiocytic reticulosarcoma.

Topics: Acid Phosphatase; Diagnosis, Differential; Esterases; Glucuronidase; Histiocytes; Humans; Leukemia, Lymphoid; Leukemia, Myeloid, Acute; Lymphocytes; Lymphoma; Lymphoma, Large B-Cell, Diffuse

Ninety diffuse large-cell lymphomas (diffuse histiocytic lymphoma) were subclassified into B-cell, T-cell, and histiocytic types according to their enzyme histochemical and immunohistochemical characteristics. The B-cell type was characterized by presence of intracellular monoclonal immunoglobulin; negative or weakly positive diffuse acid phosphatase activity; and an occasional focal nodular pattern or preceding nodular lymphoma. The T-cell type was characterized by moderate, focal acid phosphatase activity; convoluted nuclear structure; and frequent preceding cutaneous manifestations. The histiocytic type was characterized by strong nonspecific esterases and diffuse acid phosphatase activity and presence of lysozyme and phagocytic activity. Most of the lesions (74 cases) were of the B-cell type. This group was further subdivided into follicular center cell type and B-cell immunoblastic sarcoma, according to the stage of cellular transformation. Preliminary clinical correlation suggests that the histiocytic type is most resistant to treatment. B-cell immunoblastic sarcomas were much more aggressive than neoplasms of the follicular center cell type.

Topics: Acid Phosphatase; Adult; Aged; B-Lymphocytes; Esterases; Female; Follow-Up Studies; Frozen Sections; Glucuronidase; Histiocytes; Histocytochemistry; Humans; Immunoglobulins; Lymphocyte Activation; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Macrophages; Male; Middle Aged; Mortality; Receptors, Antigen, B-Cell; Rosette Formation; Staining and Labeling; T-Lymphocytes

Four group systems of serum proteins (Hp, Gc, Gm, Km) and five group systems of erythrocyte enzymes (AP, PGM1, GPT, AK, EsD) were determined in 63 patients with malignant lymphoma. Statistical analysis of the distribution of the above mentioned systems in patients and Polish population samples did not reveal any significant differences, which points to the lack of any correlation between the disease and the group systems under examination.

Topics: Acid Phosphatase; Adenylate Kinase; Adolescent; Adult; Aged; Alanine Transaminase; Blood Group Antigens; Blood Proteins; Erythrocytes; Esterases; Female; Hodgkin Disease; Humans; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Male; Middle Aged; Phosphoglucomutase

Topics: Acid Phosphatase; Animals; Cathepsins; Cell Line; Collagen; Endotoxins; Escherichia coli; Glucuronidase; Hydrolases; Lymphoma, Large B-Cell, Diffuse; Macrophages; Mice; Sarcoma, Experimental

Topics: Acid Phosphatase; B-Lymphocytes; Glucuronidase; Humans; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Periodic Acid-Schiff Reaction; T-Lymphocytes

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adult; Aged; Alkaline Phosphatase; Esterases; Female; Glucuronidase; Humans; Immunoenzyme Techniques; Leukemia, Hairy Cell; Leukemia, Lymphoid; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Male; Middle Aged; Mycosis Fungoides; Nucleotidases; Rosette Formation

The enzyme activity for alpha-naphthyl-acetate esterase, naphthol-AS-acetate esterase, naphthol-AS-D-chloroacetate esterase, acid phosphatase, L(+)-tartrate-resistant acid phosphatase, adenosine triphosphatase, and 5'-nucleotidase was examined on the neoplastic cells of giant follicular lymphoblastoma, the so-called reticulum cell sarcoma and Sézary syndrome. The neoplastic cells of giant follicular lymphoblastoma showed distinct activity for adenosine triphosphatase and 5'-nucleotidase, and those of the so-called reticulum cell sarcoma had no characteristic nature of the reticulum cells or histiocytes enzyme histochemically. These findings suggest that these neoplastic cells may be derived from the B-cell system. In Sézary syndrome, acid phosphatase activity was localized in a small paranuclear area in Sézary cells, which were considered to have a T-cell nature. It is thought that these enzyme histochemical methods are easy and useful in differentiating the B- or T-cell nature and the classification of non-Hodgkin's lymphomas.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Adult; Aged; Esterases; Female; Histocytochemistry; Humans; Lymphatic Diseases; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Male; Middle Aged; Naphthaleneacetic Acids; Naphthol AS D Esterase; Nucleotidases

The malignant non-Hodgkin's lymphomas of childhood are of highgrade malignancy only. There are lymphoblastic and immunoblastic forms. The lymphoblastic lymphomas can be of the Burkitt type (B-cell-derived) or of the "convoluted" or acid phosphatase type (T-cell-derived). A larger number of the lymphoblastic lymphomas are "unclassified" and usually do not belong to either the B-or the T-cell system (stem-cell-derived?). Most of the immunoblastic lymphomas (previously called "reticulosarcomas") are derived from the B-cell series. Besides the lymphomas in the actual sense, there are also true histiocytic reticulosarcomas of childhood. Hodgkin's disease is probably more common in childhood than all of the non-Hodgkin's lymphomas combined.

Topics: Acid Phosphatase; Adolescent; Age Factors; B-Lymphocytes; Burkitt Lymphoma; Child; Child, Preschool; Female; Germany, West; Humans; Infant; Leukemia, Lymphoid; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Male; T-Lymphocytes

Topics: Acid Phosphatase; Adult; Animals; B-Lymphocytes; Cell Nucleus; Complement C3; Female; Humans; Immunoglobulins; Leukemia, Lymphoid; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Male; Middle Aged; Rosette Formation; Sheep; T-Lymphocytes

Topics: Acid Phosphatase; Clone Cells; Humans; Immunoglobulin Fragments; Immunoglobulin M; Isoelectric Focusing; Leukemia; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Receptors, Antigen, B-Cell; Rosette Formation

In exsudate cells separated from serous body cavities of 29 tumour patients and 30 patients with inflammatory and congestive effusion in cardiac failure or liver cirrhosis respectively the activities of acid and alkaline phosphatase were determined. In addition to sudanophilia the cell content of glycogen and that of ribonucleinic acid were evaluated. By means of cytochemical findings it could be found that an increase of unspecific esterase, acid phosphatase and ribonucleic acid in atypical cells points to a malignous ethiology of the exudate.

Topics: Acid Phosphatase; Alkaline Phosphatase; Ascitic Fluid; Esterases; Exudates and Transudates; Glycogen; Heart Failure; Histocytochemistry; Hodgkin Disease; Humans; Leukemia; Liver Cirrhosis; Lymphoma, Large B-Cell, Diffuse; Neoplasms; Peritonitis; Pleural Effusion; Pleurisy; RNA

Topics: Acid Phosphatase; Alkaline Phosphatase; Connective Tissue; Connective Tissue Cells; DNA; Enzymes; Esterases; Fibroma; Fibrosarcoma; Granulation Tissue; Histiocytoma, Benign Fibrous; Histocytochemistry; Humans; Leprosy; Leucyl Aminopeptidase; Lymphoma, Large B-Cell, Diffuse; Mycosis Fungoides; Skin; Skin Diseases; Skin Neoplasms; Tuberculosis, Cutaneous

26 cases of malignant lymphomas and 23 other lymphoreticular conditions were investigated enzyme histochemically. Each type of malignant lymphoma revealed a different enzyme histochemical pattern characteristic of its type. These features are not only applicable to differential diagnosis but also suggest clues to the understanding of histogenesis and nature of malignant lymphomas.

Topics: Acid Phosphatase; Esterases; Glucuronidase; Histocytochemistry; Hodgkin Disease; Humans; Hyperplasia; Isoenzymes; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Multiple Myeloma; Naphthols

Topics: Acid Phosphatase; Adolescent; Adult; Arylsulfatases; Cell Count; Child; Child, Preschool; Eosinophils; Female; Hodgkin Disease; Humans; Inflammation; Lymph Nodes; Lymphoma, Large B-Cell, Diffuse; Male; Middle Aged; Spleen

Topics: Acid Phosphatase; Adult; Aged; Aneuploidy; Animals; Ascitic Fluid; Biopsy; Cell Line; Child; Esterases; Female; Herpesvirus 4, Human; Histocytochemistry; Humans; Immunoglobulins; Karyotyping; Lipids; Lymph Nodes; Lymphoma, Large B-Cell, Diffuse; Male; Mice; Mice, Inbred Strains; Neoplasm Transplantation; Pleural Effusion; Staining and Labeling; Transplantation, Heterologous; Vacuoles

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Bone Neoplasms; Carcinoma; Esterases; Glucuronidase; Histocytochemistry; Hodgkin Disease; Humans; Leukemia, Lymphoid; Leukemia, Myeloid; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Monoamine Oxidase; Multiple Myeloma; Neoplasm Metastasis; Neuroblastoma; Plasmacytoma; Sarcoma, Ewing

Topics: Acid Phosphatase; Adult; Brain Neoplasms; Central Nervous System Diseases; Cerebrospinal Fluid; Child, Preschool; Epilepsy; Female; Glioblastoma; Humans; Hypersensitivity; Inflammation; Lymphoma, Large B-Cell, Diffuse; Lysosomes; Male; Mental Disorders; Neurotic Disorders; Peptide Hydrolases; Psychotic Disorders; Vascular Diseases

Topics: Acid Phosphatase; Alkaline Phosphatase; Breast Neoplasms; Clinical Enzyme Tests; Diagnosis, Differential; Esophageal Neoplasms; Hodgkin Disease; Humans; Leukocytes; Lung Neoplasms; Lymphoma, Large B-Cell, Diffuse; Rectal Neoplasms; Stomach Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Esterases; Glucuronidase; Histocytochemistry; Hodgkin Disease; Humans; L-Lactate Dehydrogenase; Lectins; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Leukocytes; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Multiple Myeloma; Peroxidases; Sarcoma

Topics: Acid Phosphatase; Bone Marrow; Diagnosis, Differential; Hodgkin Disease; Humans; Isoenzymes; Liver; Lymphatic Diseases; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Spleen

Topics: Acid Phosphatase; Alkaline Phosphatase; Bilirubin; Colonic Neoplasms; Esophageal Neoplasms; Humans; Kidney Neoplasms; Leukemia, Myeloid; Liver Neoplasms; Lung Neoplasms; Lymphoma, Large B-Cell, Diffuse; Male; Neoplasms; Pancreatic Neoplasms; Phosphatidylcholines; Phosphatidylethanolamines; Phosphatidylinositols; Phospholipids; Prostatic Neoplasms; Rectal Neoplasms; Sphingolipids; Stomach Neoplasms; Thyroid Neoplasms; Triglycerides; Urinary Bladder Neoplasms

Topics: Acid Phosphatase; Alkaline Phosphatase; Bone Neoplasms; Histocytochemistry; Humans; Lymphoma, Large B-Cell, Diffuse; Osteosarcoma; Sarcoma, Ewing

Topics: Acid Phosphatase; Cell Differentiation; Esterases; Hodgkin Disease; Humans; Lymph Nodes; Lymphocytes; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin

Topics: ABO Blood-Group System; Acid Phosphatase; Adult; Alkaline Phosphatase; Blood Group Antigens; Brazil; Breast Neoplasms; Child; Diet; Electrophoresis; Female; Genetics, Medical; Hawaii; Humans; Intestines; Isoenzymes; Lewis Blood Group Antigens; Liver Cirrhosis; Lymphoma, Follicular; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Male; Phosphoric Monoester Hydrolases; Placenta; Pregnancy; Saliva; Stomach Neoplasms; Sweden; Tartrates

Topics: Acid Phosphatase; Alkaline Phosphatase; Bone Neoplasms; Chondroma; Chondrosarcoma; Diagnosis, Differential; Giant Cell Tumors; Hemangiosarcoma; Histocytochemistry; Humans; Lymphoma, Large B-Cell, Diffuse; Methods; Osteoma, Osteoid; Osteosarcoma; Sarcoma, Ewing; Sarcoma, Synovial

Topics: Acid Phosphatase; Astrocytoma; Brain Chemistry; Brain Neoplasms; Cytoplasm; Ependymoma; Esterases; Glioblastoma; Histocytochemistry; Humans; Lymphoma, Large B-Cell, Diffuse; Medulloblastoma; Neoplasm Metastasis; Neurilemmoma; Phosphates; Physostigmine

Topics: Acid Phosphatase; Antibodies; Glucuronidase; Humans; Leukemia, Lymphoid; Lymphocytes; Lymphocytosis; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin

Topics: Acid Phosphatase; Alkaline Phosphatase; Aminopeptidases; Bone Marrow; Bone Marrow Cells; Carcinoma; Clinical Enzyme Tests; Cytodiagnosis; Diagnosis, Differential; Esterases; Humans; In Vitro Techniques; Lymph Nodes; Lymphoma, Large B-Cell, Diffuse; Neoplasms

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alkaline Phosphatase; Animals; Dihydrolipoamide Dehydrogenase; Esterases; Hematopoietic System; Histocytochemistry; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Leukemia, Experimental; Lymphoma, Large B-Cell, Diffuse; Mice; Mononuclear Phagocyte System; Neoplasm Transplantation; Nucleotidases; Sarcoma, Experimental; Succinate Dehydrogenase

Topics: Acid Phosphatase; Histocytochemistry; Hodgkin Disease; Humans; Isoenzymes; Lymph Nodes; Lymphadenitis; Lymphatic Metastasis; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Neoplasms; Sarcoma; Syphilis; Tartrate-Resistant Acid Phosphatase

Topics: Acid Phosphatase; Adenoma; Brain Abscess; Brain Neoplasms; Cerebral Cortex; Ependymoma; Glioma; Histocytochemistry; Humans; Lymphoma, Large B-Cell, Diffuse; Medulloblastoma; Meningioma; Microscopy, Fluorescence; Pituitary Neoplasms; Staining and Labeling

Topics: Acid Phosphatase; Anemia; Anemia, Aplastic; Azo Compounds; Blood Cells; Bone Marrow Cells; Histocytochemistry; Hodgkin Disease; Humans; Leukemia; Leukemia, Lymphoid; Leukemia, Myeloid; Lymphoma; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Multiple Myeloma; Mycosis Fungoides; Neoplasms; Polycythemia Vera; Sarcoma