acid-phosphatase and Lymphocytosis

Topics: Acid Phosphatase; DNA; Electron Transport; Humans; Infectious Mononucleosis; Leukemia; Leukemia, Lymphoid; Leukocytes; Lymphocyte Activation; Lymphocytes; Lymphocytosis; RNA

We have studied the morphology and cytochemistry in relation to the immunological phenotyping and functional properties of T cells from eight patients with chronic T gamma lymphocytosis. At the light microscopic level the morphology of the patients' lymphocytes was similar to that described for large granular lymphocytes. Ultrastructurally, a division into two groups could be made on differences in the amount of cytoplasm and the location and the more irregular form of the nuclei. The lymphocytes of one group (five patients) had in common the phenotype Fc gamma +, T3 +, T4 -, T8 +, Ia -, M1 - and demonstrated (with the exception of one patient) the same functions: presence of K-cell activity, absence of NK, helper and suppressor cell activities. In the other group (three patients), the lymphocytes of one patient showed the same phenotype and functions as those indicated above. The other two patients both lacked the T8 antigen on their lymphocytes but were different with regard to other surface markers. In addition, their cells were functionally identical: both demonstrated NK- and K-cell activity. Thus in this group of eight patients with chronic T gamma lymphocytosis, the immunological and functional subdivision paralleled in part a morphological division at the ultrastructural level.

Topics: Acid Phosphatase; Adult; Aged; Antigens, Differentiation, T-Lymphocyte; Antigens, Surface; Cell Nucleus; Esterases; Female; Humans; Lymphocytosis; Male; Microscopy, Electron; Middle Aged; Receptors, Fc; Receptors, IgG; Rosette Formation; T-Lymphocytes

Morphological, immunologic, and functional properties of peripheral blood cells from two patients with chronic proliferations of granular lymphocytes are described. Cells from both patients showed a heterogeneous pattern from both a morphological and immunologic standpoint, indicating a polyclonal, rather than a monoclonal, expansion of these cells. In fact, both large and small-to-medium-sized granular lymphocytes were observed, and different percentages of positivity were found in the analysis with a large panel of monoclonal antibodies. Serologic and histologic features support the hypothesis that this lymphocytosis could be secondary to bacterial or viral infections rather than a primary event, suggesting that these patients may have chronic reactive immunoregulatory disorders.

Topics: Acid Phosphatase; Adult; Antigens, Viral; Bone Marrow Cells; Carrier State; Cytoplasmic Granules; Glucuronidase; Hepatitis B Antigens; Hepatitis, Chronic; Hepatomegaly; Herpesvirus 4, Human; Humans; Hypergammaglobulinemia; Lymphocytes; Lymphocytosis; Male; Middle Aged; Monocytes; Naphthol AS D Esterase; Salmonella Infections

Topics: Acid Phosphatase; Chronic Disease; Female; Humans; Leukemia, Lymphoid; Lymphocytes; Lymphocytosis; Male

Topics: Acid Phosphatase; Aged; Alkaline Phosphatase; Bone Marrow; Esterases; Female; Histocytochemistry; Humans; Leukemia, Lymphoid; Lymphocytes; Lymphocytosis; Reticulocytes; Skin; Skin Manifestations

Topics: Acid Phosphatase; Antibodies; Glucuronidase; Humans; Leukemia, Lymphoid; Lymphocytes; Lymphocytosis; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin